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토끼 수정란에서 Green Fluorescent Protein 유전자의 발현 KCI 등재

Expression of Green Fluorescent Protein(GFP) Gene in Rabbit Embryos

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한국동물생명공학회지 (구 한국수정란이식학회지) (Journal of Animal Reproduciton and Biotechnology)
한국동물생명공학회(구 한국수정란이식학회) (Journal of Animal Reproduction & Biotechnology)
초록

The efficiency of transgenic livestock animal production may be improved by early selection of transgenci preimplantation embryos. To examine the possibility of GFP gene as a non-invasive marker for the early screening of transgenic embryo, the GFP gene was microinjected into rabbit zygotes and the later stages of preimplantation embryos were examined for the expression of GFP. The presence of injected DNA was detected by PCR analysis and the expression of GFP was detected by observing green fluorescence in embryos under a fluorescent microscope. Out of 108 GFP gene-injected rabbit zygotes, seventy three(67.6%) were fluorescence-positive. When 11 fluroresecence-positive blastocysts were analyzed for the presence of GFP gene by PCR, 6(54.5%) were positive, and all of the 8 flrouescence-negative blastocysts were also negative by PCR. The results indicate that the screening of transgene in rabbit embryos by PCR analysis and GFP detection could be a promising method for the preselection of transgenic embryos.

저자
  • 강태영(경상대학교 수의과대학, 동물의학연구소)
  • 윤희준(경상대학교 수의과대학, 동물의학연구소)
  • 채영진(서울대학교 수의과대학)
  • 이항(서울대학교 수의과대학)
  • 이효종(경상대학교 수의과대학, 동물의학연구소)