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pp.79-89
배분화과정시 나타나는 변화에 미치는 의 영향을 알아보고자 whole cell voltage clamp 기법, 방사선 등위원소 면역측정법, 그리고 공초점 현미경을 통하여 처리 후 나타나는 전류 변화 및 세포내 농도 변화를 조사하였다. 생쥐의 미성숙 난자는 난소의 난포를 천자하고, 배란난자는 과배란 처리 후 난관에서 회수하였다. 수정란은 과배란 처리 후 수컷 생쥐와 교미를 유도한 후 각각의 단계에 맞는 수정란을 채란하였다. 혈중 의 농도는 심장을 천자하여
Steroid hormones control the expression of many cellular regulators, and a role thor estrogen in mouse oocytes has been well documented. The preovulatory increment is generally accepted as the endocrine process regulating induction of in vivo oocyte maturation To address whether the activity of the T-type channel is altered by 17 beta-estradiol ( ), we examined the actions of on the calcium channel of mouse oocytes and early embryos. Oocrtes were collected from the oviduct of mice treated with pregnant mare's serum gonadotropin (PMSG) and human choronic gonadotropin (hCG). Whole cell voltage clamp technique and confocal microscopy were used to examine that increase intracellular concentration ([C ] ) via voltage dependent channel (VDC) and estrogen receptor (FSR), and concentration by the use of radioimmunoassay (RIA) were examined in mouse. The results obtained were as follows: The peak of current induced by increased 122% to 1.500.03 nA from 1.230.21 nA (n=15) in the presence of 5 mM extracellular concentration ([C ] ). The increased current was temporally associated with transients. The intracellular level increased 207%~30 s following the addition of 1(relative fluorescence intensity: 836.4131.2 for control, n=10, 1736.4192.0 in the presence of , n=10). increased amplitude of current and [C ] . -induced current and concentration in blood were showed difference on the stage of embryo. These results suggest that modulate channel to increase influx. influx.
