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산소농도 및 배양액이 돼지 난포란의 체외성숙과 배발달에 미치는 영향 KCI 등재

Effects of Oxygen Concentrations with Different Media on In Vitro Maturation and Development of Porcine Follicular Oocytes

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한국동물생명공학회지 (구 한국수정란이식학회지) (Journal of Animal Reproduciton and Biotechnology)
한국동물생명공학회(구 한국수정란이식학회) (Journal of Animal Reproduction & Biotechnology)
초록

본 연구는 돼지 난포란의 체외성숙에 적합한 배양액을 조사하고, 아울러 산소농도가 돼지 난포란을 이용한 체외수정란의 생산에 미치는 영향을 구명하고자 실시하였다. 본 연구에서 얻어진 결과를 요약하면 다음과 같다. 1. NCSU-23과 TCM-199 배양액에 10% PFF를 첨가하여 5% 및 20% 산소조건하에서 체외성숙을 유기한 결과, 배양액 및 산소조건에 따른 난핵붕괴률 및 핵성숙률에는 유의적 (P>0.05)인 차이가 없었다. 2. NCSU-23이 TCM

The present study was carried out to examine the effects of concentrations and culture media on in vitro maturation and embryo development of porcine follicular oocytes. The results were summarized as fellows : 1. The rates of GVBD and nuclear maturation in NCSU-23 and TCM-199 media with 10% PFF under the conditions of 5% and/or 20% concentrations were not different among the each treatment groups(P>0.05). 2. The rates of polyspermy and mean numbers of penetrated sperm were significantly lower in NCSU-23 medium than in TCM-199 medium (P>0.05). However, the rates of polyspermy and mean numbers of penetrated sperm were not different between 5% and 20% concentrations. 3. The rates of blastocyst formation at day 7 after in vitro fertilization were significantly higher in NCSU-23 medium under the condition of 20% concentration than in TCM-l99 medium under the condition of 5% or 20% concentrations. However, the rates of blastocyst formation and total cell numbers in blastocysts were not different between 5% and 20% concentrations. In conclusion, the use of NCSU-23 medium under the condition of 20% concentration was beneficial for porcine oocyte maturation and in vitro development.

저자
  • 한만희(충남대학교 농업생명과학대학 동물자원학부)
  • 이경본(충남대학교 농업생명과학대학 동물자원학부)
  • 박병권(공주문화대학)
  • 박창식(서길웅)
  • 이규승(충남대학교 농업생명과학대학 동물자원학부)