논문 상세보기
pp.203-209
본 실험은 원시난포의 체외배양 체계를 확립할 수 있는 가능성을 검토하기 위해 10일과 20일령 마우스의 난소에서 분리한 난포를 체외배양하여 난포의 형태변화, 난자의 성장를 및 난자의 핵성숙단계를 조사하여 비교하였다. 그 결과를 요약하면 다음과 같다. 1. 10일령과 20일령의 마우스에서 분리한 난포는 배양 6일째부터 난포강이 형성되었다. 2. 10일령과 20일령 마우스의 난소에서 분리한 난포의 수는 평균 21.5와 33.3개였고, 배양하기 전에 투명대를
The aim of this study was to assess the developmental capacity of oocytes maturated in vitro after 10 days of culture when the preantral follicles were isolated from juvenile mice 10- and 20-day old, respectively, and to develop in vitro culture system that observed a view to morphology of follicles and nucleus maturation of oocytes. The antral-like cavities became formation after 6 days of culture in follicle isolated from 10- and 20-day old mice. The number of follicles were 21.5 and 33.3 in ovary isolated from 10- and 20-day old mice, respectively. The diameters of oocytes were 51.85 and 57.50 before culture and were grew 55.95 and 63.11 after culture for 10 days, in follicles isolated from 10- and 20-day old mice, respectively. The observation rates up to the M II and from GV to M II were 4.3 and 22.1%, and 14.5 and 61.1% after culture for 10 days in follicles isolated from 10- and 20-day old mice, respectively,
