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Vitrification법에 의한 돼지 난자의 동결-응해 후 생존능력에 있어서 동해보호제와 Superoxide Dismutase의 영향 KCI 등재
Effect of Superoxide Dismutase and Cryoprotectants on Viability of Frozen-thawed Porcine Oocytes by Vitrification Method
pp.179-185
본 연구에서는 vitrification방법을 사용하여 돼지 미수정란의 동결-융해시 난자생존능력에 대한특정 동해방지제 사용과 superoxide dismutase(SOD) 첨가의 영향을 검토하고자 수행하였다. 그 결과 미성숙 난자를 ethylene glycol과 DMSO 노출 후 성숙율(M-I에서 19.9%)이 glycerol과 DMSO 노출 후 성숙율(M-I에서 6.5%)보다 더 높았으며 ethylene glycol에 노출 후에는 M-I기로 성숙발달한
This study was performed to investigate the effect of different cryoprotectants and superoxide dismutase(SOD) on viability of frozen-thawed oocytes by vitrification method in the pig. The proportions of oocytes matured to metaphase-I stage were higher in medium with ethylene glycol and DMSO(19.9%) than in medium with glycerol and DMSO(6.5%). When the oocytes were exposed in medium containing ethylene glycol, oocyte matured to prophase-I were not observed. On the other hand. significant differences were not observed between in medium with and without SOD( 1 unit/) during IVM of vitrified and thawed immature oocytes. However, the maturation rate from metaphase-I to metaphase-II were higher in medium with that than without SOD. The penetration rates after IVF of oocytes frozen-thawed were also higher in medium with that than without SOD. These results indicate that frozen-thawed oocytes treated with ethylene glycol and DMSO was more protective against freezing effect and that addition of 1 unit/ SOD in medium fur prevent of lipid peroxidation may play a positive role in improving of viability of frozen-thawed oocytes.
