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Hydrocortisone 투여가 비유중기 재래산양의 유단백질과 유선세포 Prolactin Receptor mRNA 발현에 미치는 영향 KCI 등재

Effects of Hydrocortisone Administrations on Expressions of Casein and Prolactin Receptor mRNAs in Mammary Glands of Mid-Lactation of Korean Goats

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한국동물생명공학회지 (구 한국수정란이식학회지) (Journal of Animal Reproduciton and Biotechnology)
한국동물생명공학회(구 한국수정란이식학회) (Journal of Animal Reproduction & Biotechnology)
초록

Glucocorticoid는 비유기 동물의 유선세포 pro-lactin receptor(PRL-R) 발현을 증가시키며, 전반적인 유선세포의 유합성 작용을 활성시킴으로 유합성 능력을 증진시킨다. 유선세포 PRL-R 발현량 증가는 유생산량 향상과 밀접한 관계를 갖는다. 본 실험은 비유중기의 재래산양의 유합성 능력을 향상시키고자, 0.05. 0.1과 0.2 g hydrocortisone을 5ml의 생리식염수에 현탁하여, 정맥투여하고 유선세포 PRL-R와 -유

Glucocorticoid is activating mammary gland cells for lactating animals, resulting in increasing abilities of the milk synthesis. Expression of the prolactin receptor(PRL-R) in mammary gland cells was closely associated with milk production. To increase lactation ability for the Korean Native Goats at mid-lactation period. 0.05, 0.1. and 0.2 g of hydrocortisone was administrated with 5 of saline. and injected into vein. For the control, 5 of saline was administrated in to vein. After 24 H, the mammary gland tissue was collected, and mRNA expression rates were investigated for the alpha-casein and PRL-R using competitive PCR(polymerase chain reaction). There was no significant differences between treatment and control groups for the mRNA expression rate of PRL-R in mammary gland cells after 24 h of administration of hydrocortisone. The rate of mRNA expression for the alpha-casein was increased 37%, 630%, and 380% at 0.05, 0.1, and 0.2 g of hydrocortisone administration groups, respectively, comparing with control group. The results suggested that PR L-R mRNA expression of mammary gland cell by administration of hydrocortison was not significant, but increase of the alpha-casein mRNA expression my be differences of expression of functional proteins in the cell and expression patterns of protein secretion time to out of the cell. This study showed increase of alpha-casein mRNA expression by administration of hydrocortisone at mid-lactation period of Korean native goat.

저자
  • 전기준(축산기술연구소 대관령지소)
  • 김재영(한국화학시험연구원)
  • 최재관(축산기술연구소 대관령지소)
  • 정영훈(축산기술연구소 대관령지소)
  • 박정준(축산기술연구소 대관령지소)
  • 이용준(축산기술연구소 대관령지소)
  • 우제석(축산기술연구소 대관령지소)
  • 서동석(한국화학시험연구원)
  • 홍승국(한국화학시험연구원)