본 연구는 돼지 난포란의 vitrification 동결 시 내동제의 종류 및 농도가 생존율에 미치는 영향과 수정 후 체외발생율을 조사하고자 수행하였다. 1.0, 15, 30 및 40시간 성숙 배양시킨 난포란을 vitirfication 동결보존 후의 MII로의 발생율은 각각 였으며, diploid로의 발생율은 로서 대조군의 ME 단계의 에 비해 낮게 나타났으며 diploid 단계의 에 비해서는 높은 체외성숙율을 나타냈다. 체외발생율은 초기의 미숙 난포란일

This study examines the effects of kinds and concentrations of cryoprotectants on the survival rate of vitrification-thawed porcine oocytes, together with the effects on survival, in vitro fertilization and development of immature oocytes. 1. The developmental rate of oocytes to MII and diploid stage when the vitrification-thawed of recovered immature oocytes cultured for 0, 15, 30 and 40h were cultured for 0, 15, 30 and 40h were , respectively. The in vitro development to MII stage were lower than the control group , but higher fo. diploid stage . 2. When the vitrification of immature oocytes after being culture for 0, 15, 30 and 40 hours, the survival rate were respectively. This result was lower than that of the control group . 3. When the fertilization of the vitrified immature oocytes after being culture for 0, 15, 30 and 40 hours, the in vitro fertilization rate were , and developmental rates were , respectively. This results were lower than the control group . 4. When the fertilization of the immature oocytes after being culture for hours vitrified with EDS and ETS, the fertilization and developmental rates were and , respectively. This results were lower than the control group .

• 임정훈
• 전연화(충남대학교 수의과대학)
• 이규승
• 김상근(충남대학교 수의과대학)