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소 체외 수정란의 체외 발육에 미치는 Prostaglandins의 영향 KCI 등재

Effects of Prostaglandins on In Vitro Development of Bovine Embryos

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한국동물생명공학회지 (구 한국수정란이식학회지) (Journal of Animal Reproduciton and Biotechnology)
한국동물생명공학회(구 한국수정란이식학회) (Journal of Animal Reproduction & Biotechnology)
초록

본 연구는 소 체외 수정란의 팽창 및 hatching시 prostaglandin 와 prostaglandin 의 영향에 대하여 검토하였다. 체외 수정란의 체외 배양시 다음과 같이 (1) 0, 1, 10 및 100ng/ml , (2) 0, 1, 10 및 100ng/ml , (3) low (1ng/ml : 1ng/ml), (4) low , (5) high (10ng/ml : 1ng/ml), (6) high : high (10ng/ml : 10ng/ml)로

This study was conducted to examine the effects of prostaglandin and prostaglandin on the expansion and hatching of bovine embryos. During the in vitro culture, embryos were cultured with the following groups: (1) 0, 1, 10 and 100ng/ml (2) 0, 1, 10 and 100 ng/ml , (3) low concentration of ; low concentration of , (1ng/ml : 1ng/ml), (4) low concentration of : high concentration of (1ng/ml : 10ng/ml) (5) high concentration of : low concentration of (10ng/ml 1ng/ml) (6) high concentration of : high concentration of (10 ng/ml : 10ng/ml). In the results of this study, treatment of or did not affect in vitro development to blastocysts. However, the hatching rates of embryos cultured with 10ng/ml (10.3%) and 1ng/ml 10ng/ml (22.2%) were significantly (P<0.05) higher than in control (4.3% and 12.7%) and other treatment groups. All groups treated with high concentrations of showed decreased hatching rates. Thus, this results suggested that were concerned with the hatching in bovine embryos, and their effects on hatching were different by the concentrations.

저자
  • 신승오(강원대학교 동물생명과학대학)
  • 박수봉(축산연구소)
  • 박춘근(강원대학교 동물생명과학대학)