논문 상세보기
pp.149-154
임신의 성립 및 유지에 중요한 역할을 하는 자궁내막세포에 통로의 존재를 확인하기 위하여 본 연구를 수행하였다. 통로는 일반적으로 중추신경계에 풍부하게 존재하면서 세포의 안정막 전압을 유지시킨다. 역전사 중합 효소 중합 반응과 면역 세포 화학 염색 방법을 이용하여 자궁내막세포에 존재하는 통로를 조사한 결과, TASK-1, TASK-3, TREK-1, TREK-2 및 TRAAK의 발현이 확인되었다. TASK-3와 TREK-1은 핵을 포함한 세포 전역에 발현
Endometrial cells play important roles in implantation and during pregnancy. This study was carried out to identify whether two-pore domain channels are expressed in endometrial cells of Korean cattle. channels set the resting membrane potential of many kinds of neuronal cells in the central and peripheral nervous systems. RT-PCR data showed that TASK-1, TASK-3, TREK-1, TREK-2, and TRAAK were expressed in bovine endometrial cells, and the mRNA expression levels were similar between endometrial cells with or without endometritis. The protein expression was confirmed by using commercially available polyclonal antibodies (TASK-3, TREK-1, TREK-2, and TRAAK). TASK-3 and TREK-1 were expressed in all area of endometrial cells including nuclei, while TREK-2 and TRAAK were expressed in all area of cells except nuclei. These results demonstrate for the first time the presence of channel in endometrial cells of Korean cattle.
