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난소낭종 및 자궁내막염 한우에서 염증유래 유전자 발굴 KCI 등재

Identification of Inflammation-related Genes Altered in the Cystic Ovary and Endometritis of Korean Cattle

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  • URLhttps://db.koreascholar.com/Article/Detail/47576
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한국동물생명공학회지 (구 한국수정란이식학회지) (Journal of Animal Reproduciton and Biotechnology)
한국동물생명공학회(구 한국수정란이식학회) (Journal of Animal Reproduction & Biotechnology)
초록

This study was carried out to investigate inflammation-related gene expression altered in ovary and endometrium of Korean cattle with reproductive disorders using microarray. In the present study, nine inflammation-related differential1y expressed genes (DEGs) were identified in the cystic ovary and endometrium with endometritis. In the follicular cyst, eotaxin and alpha-2-HS-glycoprotein (AHSG) were up-regulated, whereas complement component 3 (C3) and oxidised low density lipoprotein (lectin-like) receptor 1 (OLR1) were down-regulated. Complement component 4A (C4A) was up-regulated in luteal cyst. In the endometritis, chemokine 1igand l and 2 (CXCL1 and CXCL2), protein C (inactivator of coagulation factors Va and VIIIa), and complement component C5 were up-regulated, whereas kininogen was down-regulated. Of these genes, we focused on eotaxin and kininogen, which were highly regulated in the follicular cyst and endometritis, respectively and on C3 commonly regulated in both reproductive disorders. The microarray data of eotaxin, kininogen, and C3 were validated by semi-quantitative PCR. Consistent with microarray data, eotaxin was up-regulated by 4-fold in the follicular cyst, while kininogen was down-regulated by 5-fold in the endometritis. C3 was down-regulated in the both follicular cyst and endometritis. Our results suggest that these inflammation-related genes could be useful markers for diagnosis of cystic ovary and endometritis of Korean cattle.

저자
  • 최창용(농촌진흥청 축산과학원 가축유전자원시험장)
  • 박선영(경상대학교 의과대학 생리학교실)
  • 김은숙(경상대학교 의과대학 생리학교실)
  • 문윤자(경상대학교 의과대학 생리학교실)
  • 박혜진(경상대학교 의과대학 생리학교실)
  • 손동수(농촌진흥청 축산과학원 가축유전자원시험장)
  • 조상래(농촌진흥청 축산과학원 가축유전자원시험장)
  • 김현종(농촌진흥청 축산과학원 가축유전자원시험장)
  • 김재범(농촌진흥청 축산과학원 가축유전자원시험장)