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한우 수정란의 산소 소비량이 수청란이식 수태윷에 미치는 영향 KCI 등재

Effects of Pregnant Rate after Embryo Transfer in Oxygen Consumption of Embryos in Korean Cattle

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  • URLhttps://db.koreascholar.com/Article/Detail/47669
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한국동물생명공학회지 (구 한국수정란이식학회지) (Journal of Animal Reproduciton and Biotechnology)
한국동물생명공학회(구 한국수정란이식학회) (Journal of Animal Reproduction & Biotechnology)
초록

Oxygen consumption has been regarded as a useful indicator for assessment of mammalian embryo quality. This study was performed to investigate whether oxygen consumption reflects morphological grade of in vivo derived bovine blastocyst-stage embryos (blastocyst). The oxygen consumption of in vitro produced blastocyst was compared to its total cell number. In addition, pregnant rate was measured after transplantation of in vivo blastocysts with different oxygen consumption. The quality of blastocyst collected on day 7 after artificial insemination was categorized as grade I and II (G I and G II) based on microscopic observation of the morphology. Oxygen consumption of blastocyst was measured using a scanning electrochemical microscopy (SECM) and total cell number of in vitro blastocyst was enumerated by counting cells stained by propidium iodide. Pregnancy of recipient cow was confirmed with rectal palpation after 60 days of embryo transfer. The oxygen consumptions of G I blastocysts were significantly higher than those of G II blastocysts ( versus , p<0.05). Total cell numbers of in vitro blastocysts were 74.8, 90.7, and 110.2 in the oxygen consumption of below 10.0, 10.0~12.0, and over respectively. Total cell number was significantly increased in embryos with high oxygen consumption (p<0.05). Pregnant rate in recipient cow was 0, 50, and 85.7% in the transplantation of embryo with the oxygen consumption of below 10.0, 10.0~12.0, and over , respectively. These results suggest that measurement of oxygen consumption may help increase the pregnant rate of bovine embryos.

저자
  • 정연
  • 김재범(새란산부인과)
  • 김남태(농촌진흥청 국립축산과학원 가축유전자원시험장)
  • 최수호(경북축산기술연구소)
  • 최선호(농촌진흥청 국립축산과학원 가축유전자원시험장)
  • 연성흠(농촌진흥청 국립축산과학원 가축유전자원시험장)
  • 강다원(경상대학교 의학전문대학원 생리학교실)
  • 조상래(농촌진흥청 국립축산과학원 가축유전자원시험장)
  • 손준규(농촌진흥청 국립축산과학원 가축유전자원시험장)
  • 최창용(농촌진흥청 국립축산과학원 가축유전자원시험장)