Antibiotics supplementation in animal feeds results in bacterial resistance to the antibiotics and residue of the antibiotics in animal products, which can cause serious problems in human health. Therefore, the finding of new substances replacing antibiotics are needed. New substances are egg yolk antibody, probiotics, organic acid, mannanoligosaccharide(MOS), fructooligosaccharide(FOS), and chitosan etc. Egg yolk antibody is antibody to obtain from egg yolk of the chicken injected the specific antigen. Probiotics can prevent the problems of residue of the antibiotics and resistance to the antibiotics. Organic acids on preservation of colostrum inhibit the rottenness and increase the beneficial bacteria. MOS and FOS increase the beneficial bacteria, too. Chitosan is used the immune material to active the antibacteria.