This study were examined whether plasminogen activators (PAs) are produced by porcine fresh or frozen-thawed cumulus-oocytes complexes (COCs) and cumulus cell free-oocytes. In fresh or frozen-thawed COCs and oocytes for 0 hour cultured, no activity of PAs was detected. However, at 24 hours of culture urokinase-type plasminogen activator (uPA) was detected in COCs and denuded oocytes. In the frozen-thawed COCs and cumulus cell free-oocytes cultured for 24 hours, no PAs were observed. After COCs were cultured for 48 hours, tissue-type plasminogen activator (tPA) and tPA-PAI were observed in COCs only. In the frozen-thawed COCs and cumulus cell free-oocytes cultured for 48 hours, no PAs were observed. These results suggest that uPA, tPA and tPA-PAI are produced by porcine COCs, but only uPA by oocytes during maturation for 24 hours. Only tPA, and tPA-PAI are produced by COCs cultured for 48 hours, and no PAs are produced by denuded-oocytes cultured for 48 hours. In all of the frozen-thawed groups, no PAs are observed by COCs and denuded-oocytes.

INTRODUCTION   MATERIALS AND METHODS   RESULTS   DISCUSSION   REFERENCES

• Chen J. B.(College of Animal Resources Science, Kangwon National University)
• Sa S. J.(College of Animal Resources Science, Kangwon National University)
• Cao Y.(College of Bioengineering, Dalian University)
• Choi S. H.(Animal Genetic Resources Station, National Livestock Research Institute)
• Cheong H. T.(College of Animal Resources Science, Kangwon National University)
• Yang B. K.(College of Animal Resources Science, Kangwon National University)
• Park C. K.(Division of Animal Resourcem Kangwon National University)