This study was carried out to evaluate the effects of washing medium, breed and washing temperature of fresh and frozen-thawed boar sperm on mitochondrial activity and membrane integrity by flow cytometry. More than 80% of fresh sperm washed with mTLP-PVA medium at 20℃ exhibited an intact membrane and a functional mitochondrion. With frozen-thawed samples, a large number of sperm showed both damaged membrane (36.4~46.9%) and nonfunctional mitochondrion (55.1~71.1%) in the mTLP-PVA and BTS washing media at 20℃. There were no breed effects of fresh and frozen-thawed sperm on mitochondrial activity and membrane integrity. The percentages of damaged membrane of fresh and frozen sperm, respectively, were higher at 4℃ washing temperature than at 20℃ washing temperature in the mTLP-PVA medium. We found that washing medium and washing temperature of fresh and frozen-thawed boar sperm were important for the analyses of mitochondrial activity and membrane integrity by flow cytometry.

INTRODUCTION   MATERIALS AND METHODS   RESULTS   DISCUSSION   REFERENCES

• C. S. Park(Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University)
• Z. H. Li(Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University)
• N. D. Sung(Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University)
• D. I. Jin(Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University)
• P. Q. Cong(Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University)
• E. S. Kim(Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University)
• E. S. Song(Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University)
• Y. J. Yi(Division of Animal Science and Resources, Research Center of Transgenic Cloned Pigs, Chungnam National University)