The purpose of this study was to determine the effect of varying levels of photobiostimulation treatment dosage on plasma -endorphin concentration in humans. The subjects of this study were 21 healthy men and women, who were students of the Department of Physical Therapy, College of Health Science, Seonam University. This study was performed from October 26, 1998 to November 5, 1998. All subjects were assigned to one of three groups: a 2.0 laser group, a 4.0 laser group, an 6.0 laser group. He-Ne laser (632.8 nm wave length) and infrared laser (820 nm wave length) of three different energy densities (2.0, 4.0, and 6.0 ) were applied on the Su-Sam-Ri (L I 10) and Hab-Gog (L I 4) of acupuncture points. Blood samples were taken at pre-treatment, 30 min's post-treatment and 60 min's post-treatment. The level of endorphin was measured by radio immuno assay. The data were analyzed by descriptive statistics and repeated measure two-way ANOVA. The results of this study were as follows: 1) The human plasma -endorphin concentrations were noted to significantly increase due to the energy densities of laser photobiostimulation (p<0.05). 2) The human plasma -endorphin concentrations were noted to significantly increase during the period after laser photobiostimulation (p<0.05).