This study was conducted to examine the effect of several saccharides on the induction of capacitation and acrosome reaction (AR) and to examine the effects of mono and polysaccharides on the penetration activity of boar spermatozoa. Spermatozoa were inseminated in medium with fucose, galactose and mannose as monosaccharide, and fucoidan, galactan and mannan as polysaccharide. The penetration rates were significantly (p<0.05) lower in medium with galactose (40.6%), mannose (38.1%), fucose (41.6%) and fucoidan (36.6%) compared with control (56.7%). The rates of AR were increased (40.7 to 59.8%) by the preincubation periods prolonged from 0 to 4 hr (p<0.05). Similar tendencies were observed in AR when spermatozoa were treated with monosaccharides, but not significantly differ among the groups treated with different time of preincubation with some exception of galactose. When spermatozoa were treated with polysaccharides, the rates of AR were significantly (p<0.05) increased by preincubation time prolonged from 0 to 4 hr with an exception of fucoidan. In conclusion, the present study suggests that penetration rate of spermatozoa is higher in presence of polysaccharides than monosaccharides. Also, it may resume that the comparing to control, the all saccharides (L-fucose, D-galactose, D-mannose, fucoidan, galactan and mannan)-treated groups slightly increase the AR pattern as preincubation time prolonged.

ABSTRACT   INTRIDUSTION   MATERIALS AND METHODS    Oocytes Preparation    Treatment of Saccharides and In Vitro Fertilization    Chlortetracycline(CTC) Assessment of Spermatozoa    Evlauation of Sperm Penetration    Statistical Analysis   RESULTS   DISCUSSION   REFERENCES

• In-Sun Hwang(College of Animal Life Science)
• Hee-Tae Cheong(School of Veterinary Medicine, Kangwon National University)
• Boo-Keun Yang(College of Animal Life Science)
• Choung-Ik Kim(College of Animal Life Science)
• Choon-Keun Park(College of Animal Life Science) Corresponding author