논문 상세보기

Comparison of In Vitro Development of Porcine Embryos Derived from Trnasfer of Embryonic Germ Cell Nuclei into Oocytes by Eletrofusion and Piezo-Driven Microinjection

  • 언어ENG
  • URLhttps://db.koreascholar.com/Article/Detail/81743
구독 기관 인증 시 무료 이용이 가능합니다. 4,000원
한국동물번식학회 (The Korean Society of Animal Reproduction)
초록

Embryonic germ (EG) cells are undifferentiated stem cells isolated from cultured primordial germ cells (PGC). These cells share many characteristics with embryonic stem cells including morphology and pluripotency. Undifferentiated porcine EG cell lines demonstrating capacities of differentiation both in vitro and in vivo have been established. Since EG cells can be cultured indefinitely in an undifferentiated state, whereas somatic cells in primary culture are often unstable and have limited lifespan, EG cells may provide inexhaustible source of karyoplasts in nuclear transfer (NT). In this study the efficiencies of NT using porcine EG and fetal fibroblast cells were compared. Two different techniques were used to perform NT. With conventional NT procedure (Roslin method) involving fusion of donor cells with enucleated oocytes, the rates of development to the blastocyst stage in EG and somatic cell NT were 16.8% (59/351) and 14.5% (98/677), respectively. In piezo-driven microinjection (Honolulu method) of donor nuclei into enucleated oocytes, the rates of blastocyst formation in EG and somatic cell NT were 11.9% (15/126) and 9.4% (9/96), respectively. Regardless of NT methods used in this study, EG cell NT gave rise to comparable rate of blastocyst development to somatic cell NT. Overall, EG cells can be used as karyoplast donor in NT procedure, and embryos can be produced by EG cell NT that may be used as an alternative to conventional somatic cell NT.

목차
ABSTRACT   INTRIDUSTION   MATERIALS AND METHODS    In Vitro Maturation of Porcine Oocytes    Preparation of Porcine Fetal Fibroblasts    Isolation and Culture of Porcine EG Cells    Nuclear Transfer    Statistical Analysis   RESULTS   DISCUSSION   REFERENCES
저자
  • Kwnag Sung Ahn(Department of Physiology, Dankook University School of Medicine)
  • Ji Young Won(Department of Physiology, Dankook University School of Medicine)
  • Soon Young Heo(Department of Physiology, Dankook University School of Medicine)
  • Jee Hyun Kang(Department of Physiology, Dankook University School of Medicine)
  • Hosup Shim(Department of Physiology, Dankook University School of Medicine) Corresponding author