Studies on the Chemical Resistance of Phytopathogenic Bacteria Ⅱ. Selective Effect of Chemical Resistance on the Rice Bacterial Leaf Blight Pathogen, Xanthomonas oryzae (Uyeda et Ishiyama) Dowson, to Agrepto
벼 흰빛잎마름균을 이용하여 Streptomycin 계약제인 Agrepto에 대한 내성형질의 선발효과를 조사하여 다음과 같은 결과를 얻었다. 1) 사용된 약제 Agrepto는 에서 20분간의 온도처리에서 약제의 안정성을 보였다. 2) 서로 다른 지역균주인 75-6 및 75-9 묘주는 선발영제농도 10ug/ml 및 100ug/ml에서 상이한 내성력을 나타내는 내성균이 선발되었다. 3) 저농도인 10ug/ml 및 100ug/ml의 선발농도에서도 10,000ug/ml에서 생장이 가능한 내성균이 선발될 수 있었다. 4) 전세대 100ug/ml에서 선발하고 재차 3,000ug/ml에서 선발된 선발균주는 처리농도 100ug/ml에서 무처리와 동일한 생장량을 보였다. 5) 자연집단의 균주 75-9를 100ug/ml에서 선발한 이들 분리균주는 내성 정도에 있어서 500ug/ml 이하, 500-1,000ug/ml 및 1,000-3,000ug/ml의 내성을 나타내는 몇단계의 내성력 정도가 상이한 균들이 분포하고 있었다.
The experiments were carried out to investigate the selection effects on the drug resistance to Agrepto in Xanthomonas oryzae, the causal bacteria of rice bacterial leaf blight. The results obtained were as follows. 1, The Agrepto was stable at the heat treatment of for 20 minutes, when the drug was added in the media. 2. The local isolates of the bacteria, 75-6 and 75-9, showed the different resistante reaction, when they were selected by the Agrepto contained media which concentrations of 10r9/m1 and 100ug/ml. 3. The individuals shelving high degree of resistance, which can grow on the media contained 10,000ug/m1 of Agrepto, could selected by the concentrations as low as 10ug/ml and 100ug/m1, in one generation. 4. The highly resistant isolates which selected by 3-stepwise selections such as 100ug/ml, 3,000ug/ml and 10,000/ml plots, showed nearly normal growth at the media contained 100ug/ml of Agrepto 5. When the isolate 75-9 was selected at the 100ug/m1 of concentration, showed various degrees of resistance, indicating that the isolate may be composed of resistance groups that lower than 500ug/ml, between 500-1,000ug/ml and 1,000-3,000ug/ml, to the Agrept.