한국응용곤충학회 학술대회논문집 2014 한국응용곤충학회 추계학술발표회 , 응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry (p.10-10)

Mass Production and Diapause of Trichogramma in Dissected Eggs of Chinese Tussah Moth

키워드 :
Trichogramma dendrolimi,Antheraea pernyi,mass production,diapause induction,diapause termination,cold storage

초록

Trichogramma is widely used in biological control of many Lepidoptera insect pests in the world. Although many species of host eggs, e.g. Sitotroga cerealela, Corcyra cephalonica, Ephestia kuehniella, Mamestra brassicae and even artificial host eggs are explored in mass production of Trichogramma, eggs dissected from the ovaries in female of Chinese (Oak) tussah moth (Antheraea pernyi) are still the most popular host eggs in China. Here I introduce technology processes of Trichogramma mass production that are popularly used in China, including: 1) selecting female cocoons from mixed sexes commercial cocoons, 2) cold storage of cocoons, 3) warming up the cocoons, 4) collecting female moths and dissecting ovaries, 5) separating, washing and drying host eggs, 6) multiplying Trichogramma with the host eggs, 7) producing Trichogramma products, i.e. the host egg cards, 8) storing Trichogramma under low temperatures, 9) releasing Trichogramma in the field. Nevertheless, problems are frequently encountered in these processes, e.g. high mortality of long-term storage and multiplying too many generations etc. To solve these problems and explore a method for long-term storage, we studied the diapause of T. dendrolimi in detail. We found that the optimal processes for diapause induction were exposing hosts for parasitization at 26℃ for 8 h before keeping them at 26℃ for another 40 h, and finally moving them into 10℃ for 4 weeks. The optimal processes for diapause termination were setting the diapause Trichogramma under 1℃ for two months and then exposing them at 26℃ until their emergence.