한국응용곤충학회 학술대회논문집 2014 한국응용곤충학회 추계학술발표회 , 응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry (p.19-19)

The Plan for Activation of Insect Pheromone Industry using Molecular Technique

키워드 :
Desaturase,Fatty acid reductase,Sex pheromone production,Brewer’s yeast,Saccharomyces cerevisiae,transcriptome analysis,Genetically modified plant

초록

After firstly identified sex pheromone components of Bombxy mori, those of many insect pests were synthesized by organic chemistry methodology. These synthesized components were used for monitoring, mass trapping, and mating disruption during five decades. For identification of pheromone biosynthesis mechanisms and control to many pests bring to serious damages also were proceeded. The transcriptome analysis from pheromone glands by Next Generation Sequence (NGS) showed many genes and pathway involved on sex pheromone biosynthesis.. The two main genes involved on production of acetate and alcohol, and aldehyde from fatty acid, fatty acid desaturase and fatty acid reductase (FAR) were identified and functional characterized via gene introduction to Brewer’s yeast Saccharomyces cerevisiae. This S. cerevisiae now used as a mediator as well as cell factory for sex pheromone producing. Recently, One group was published that the plant factory for producing via genetically modified plant (tobacco, Nicotiana benthamiana) as a step of semisynthetic preparation. These trials will be suggest that firstly, the possibility of yeast as a molecular toolbox to produce pheromone components and secondly, a novel and cost-effective way of producing moderate to large quantities of pheromones with high purity and a minimum of hazardous waste.