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댕댕이나무(Lonicera caerulea)의 대장암세포 생육억제 활성 KCI 등재

Inhibitory Activity of Lonicera caerulea Against Cell Proliferation in Human Colorectal Cancer Cells

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  • URLhttps://db.koreascholar.com/Article/Detail/400303
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한국자원식물학회지 (Korean journal of plant resources)
한국자원식물학회 (The Plant Resources Society Of Korea)
초록

이상의 연구 결과로 미루어 볼 때, 댕댕이나무 잎과 가지 추추출물은 대장암 세포주 HCT116과 SW480세포의 생육을 억제 하였으나 열매추출물은 억제활성이 나타나지 않았다. 잎과 가지 추출물은 cell migration과 wound healing assay를 통해 비정상적인 세포증식 억제를 확인하였으며, β-catenin과 TCF4 의 단백질 수준을 감소시켜 비정상적인 Wnt 신호전달을 억제를 통해 대장암세포의 생육을 억제하는 것으로 판단된다. 따라서 댕댕이나무 잎과 가지는 항암을 위한 대체보완소재 및 천연 항암제 개발을 위한 소재로 활용이 가능할 것으로 판단된다.

In this study, we evaluated the effect of the extracts from Lonicera caerulea leaves (LCLE), branches (LCBE) and fruits (LCFE) on the cell growth and migration in human colorectal cancer cells, HCT116 and SW480 cells. LCLE and LCBE dose- and time-dependently inhibited the proliferation of HCT116 and SW480 cells. However, LCFE did not affect the proliferation of HCT116 and SW480 cells. In addition, LCLE and LCBE dramatically cell migration and wound healing in HCT116 cells. LCLE and LCBE decreased β-catenin protein level but not mRNA level in HCT116 and SW480 cells. Furthermore, LCLE decreased TCF4 level in both protein and mRNA level in HCT116 and SW480 cells. However, LCBE decreased TCF4 protein level but not mRNA level in HCT116 and SW480 cells. Based on these findings, LCLE and LCBE may inhibit the cell proliferation and migration through blocking Wnt signaling activation in human colorectal cancer cells. Therefore, LCLE and LCBE may be a potential candidate for the development of chemopreventive or therapeutic agents for human colorectal cancer.

목차
Abstract
서언
재료 및 방법
    실험재료
    추출물 제조
    세포 생육 억제활성 측정
    Cell migration assay
    Wound healing assay
    Reverse transcriptase-polymerase chain reaction (RTPCR)
    SDS-PAGE 및 Western blot 분석
    통계분석
결과 및 고찰
    댕댕이나무의 대장암세포 HCT116과 SW480의 세포생육에미치는 영향
    댕댕이나무 잎과 가지 추출물의 대장암 세포 HCT116과SW480의 cell migration에 미치는 영향
    댕댕이나무 잎과 가지 추출물의 대장암 세포 HCT116과SW480에서 Wnt 신호전달에 미치는 영향
적요
References
저자
  • 안미연(국립안동대학교 생약자원학과) | Mi-Yun An (Department of Medicinal Plant Resources, Andong National University)
  • 어현지(국립산림과학원 산림약용자원연구소) | Hyun Ji Eo (Forest Medicinal Resources Research Center, National Institute of Forest Science)
  • 손호준(국립산림과학원 산림약용자원연구소) | Ho-Jun Son (Forest Medicinal Resources Research Center, National Institute of Forest Science)
  • 박광훈(국립산림과학원 산림약용자원연구소) | Gwang Hun Park (Forest Medicinal Resources Research Center, National Institute of Forest Science)
  • 정진부(국립안동대학교 생약자원학과) | Jin Boo Jeong (Department of Medicinal Plant Resources, Andong National University) 교신저자