Red ginseng marc (RGM) has been used on primary industries using fertilizer or forage, and it mostly has been dumped. To improve utilization of RGM, the biological activities of RGM were examined. RGM was extracted and fractionated using various solvents and their biological activities were compared. The hexane fraction from the methanol extract of RGM (RGMMH) showed strong anti-cancer activity (58.56 ± 6.04% at 100 ㎍/mL) and anti-inflammatory effect (65.72 ± 1.33% at 100 ㎍/mL). But, oil extract of RGM extracted with hexane (RGMH) showed low activities (anti-cancer: 16.42 ± 3.33%, at 100 ㎍/mL, anti-inflammatory activity: 29.46 ± 2.10%, at 100 ㎍/mL). Their metabolites were analyzed using HPLC. Panaxydol known as anti-cancer compound of RGM was one of major compounds in RGMMH. Meanwhile, panaxydol was detected in trace amount in red ginseng marc oil (RGMH). In addition, RGMMH and RGMH showed big differences in HPLC profiling. This research suggests optimal extraction method of RGM oil.

Abstract
Introduction
Material and methods
    Extraction and solvent fractionation
    Total phenolic contents
    ABTS radical scavenging activity
    Cell culture
    Anti-inflammatory activity
    Anti-cancer activity
    Metabolite analysis
    Statistical analysis
    Anti-oxidant activity
Results
    Total phenolic contents
    Anti-inflammatory activity
    Anti-cancer activity
    Chemical profiling of RGM
Discussion
References

• Dong Gyu Lee(Department of Horticulture, Kyungpook National University)
• Ik Soon Jang(Division of Analytical Science Korea Basic Science Institute)
• Young-Hwa Kang(Department of Horticulture, Kyungpook National University) Corresponding author