Barley’s nutritional value as a health food is increasing due to its excellent nutritional functionality. In this study, the levels of β-glucan, total polyphenols, and total flavonoids were analyzed in the ethanol extracts of different barley cultivars (Hinchalssal, Heuksoojeongchal, Betaone, Ganghochung, and Saechalssal). Also, the free radical scavenging abilities of 2,2-diphenyl-1-picrylhydrazil (DPPH) and 2,2’-azino-bis-3-ethylbenzo-thiaxoline-6-sulfonic acid (ABTS) were measured to determine their antioxidant activity. The results confirmed that Betaone extract contained highly activefunctional components and exhibitedantioxidant activity. Next, we evaluated the hepatoprotective and inhibitory effects of reactive oxygen species (ROS) generated by barley ethanol extracts after inducing oxidative stress with tert-butyl hydroperoxide (tBHP) in HepG2 cells. Hinchalssal and Saechalssal extracts showed the most significant cytoprotective effect and also reduced ROS production significantly. These results suggest that Hinchalssal, Saechalssal, and Betaone represent potential natural antioxidant and hepatoprotective agents.

Abstract
서 론
재료 및 방법
    1. 실험재료
    2. 주정 추출물의 제조
    3. 이화학적 특성 분석
    4. DPPH 및 ABTS 라디칼 소거 활성 측정
    5. 세포 배양 및 세포 생존율 측정
    6. ROS 생성 억제 효과
    7. 통계분석
결과 및 고찰
    1. 보리 품종별 주정 추출물의 베타글루칸, 총폴리페놀,총플라보노이드
    2. DPPH 및 ABTS 라디칼 소거 활성
    3. HepG2 세포 생존율 및 세포 독성 보호 효과
    4. 보리 추출물의 기능 성분, 항산화 활성과 간세포 보호효과의 상관관계
    5. 활성산소종(ROS) 생성 억제 효과
요약 및 결론
References

• 양지영(농촌진흥청 국립식량과학원 작물기초기반과) | Ji Yeong Yang (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration)
• 함현미(농촌진흥청 국립식량과학원 작물기초기반과) | Hyeonmi Ham (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration)
• 이현진(농촌진흥청 국립식량과학원 작물기초기반과) | Hyun-Jin Lee (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration)
• 김현영(농촌진흥청 국립식량과학원 작물기초기반과) | Hyun Young Kim (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration)
• 우소연(농촌진흥청 국립식량과학원 작물기초기반과) | So-Yeun Woo (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration)
• 서우덕(농촌진흥청 국립식량과학원 작물기초기반과) | Woo Duck Seo (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration)
• 이미자(농촌진흥청 국립식량과학원 작물기초기반과) | Mi Ja Lee (Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration) Corresponding author