한국환경과학회지 제30권 제11호 (p.945-956)

|ORIGINAL ARTICLE|
Bacillus sp.의 생장과 인 제거에 대한 단일 및 2종 혼합 중금속의 독성 평가

Toxicity Evaluation of Single and Binary Mixture of Heavy Metals on the Growth and Phosphorus Removal Ability of Bacillus sp.
키워드 :
Toxicity assessment,Additive toxicity index,Phosphorus removal,Heavy metal,Bacillus sp.

목차

Abstract
1. 서 론
2. 재료 및 방법
   2.1. 미생물 배양 조건
   2.2. 합성 폐수
   2.3. 생장 측정 및 인산 정량
   2.4. IC50 및 EC50 추정
   2.5. 중금속 간 상호작용 평가
   2.6. 통계분석
3. 결과 및 고찰
   3.1. 단일 중금속
   3.2. 2종 혼합 중금속
4. 결 론
REFERENCES

초록

In this study, the effects of single and binary heavy metals toxicity on the growth and phosphorus removal ability of Bacillus sp.. known as be a phosphorus-removing microorganism, were quantitatively evaluated. Cd, Cu, Zn, Pb, Ni were used as heavy metals. As a result of analysis of variance of the half of inhibition concentration and half of effective concentration for each single heavy metal treatment group, the inhibitory effect on the growth of Bacillus sp. was Ni < P b < Z n < Cu < C d. A nd the inhibitory effect on phosphorus removal by Bacillus s p. w as N i < Pb < Z n < Cu < C d. When analyzing the correlation between growth inhibition and phosphorus removal efficiency of a single heavy metal treatment group, a negative correlation was found (R2 = 0.815), and a positive correlation was found when the correlation between IC50 and EC50 was analyzed (R2 = 0.959). In all binary heavy metal treatment groups, the interaction was an antagonistic effect when evaluated using the additive toxicity index method. This paper is considered to be basic data on the toxic effects of heavy metals when phosphorus is removed using phosphorus removal microorganisms in wastewater.