Bulbophyllum auricomum Lindl. is a rare orchid and has flowers with an attractive fragrance. The present study investigated the tissue culture method for micropropagation. Capsules derived from artificial self-pollination were obtained for the best seed germination in MS basal medium. Plant growth regulators (1.0 mg·L-1 of BAP and 2.0 mg·L-1 of NAA) were affected by callus induction from subcultured pseudobulb explants. For the callus subculture, different natural plant extracts were tested in 11 treatment media. Among them, MS medium with 150 mL·L-1 of coconut water was generally effective in fresh weight (1.75 ± 0.08) and (3.01 ± 0.20) of callus proliferation and PLBs induction at 1 and 2 months, respectively, followed by an MS combination of 30 g·L-1 of banana and 20 g·L-1 of potato extract. The results of a comparative study of different MS mediums containing plant growth regulators with a natural extract combination and MS medium supplemented with natural extract only showed that MS medium supplemented with a combination of natural extracts (150 mL·L-1 of coconut water) and plant growth regulators (2.0 mg·L-1 of BAP and 1.0 mg·L-1 of NAA) obtained the highest shoot regeneration (3.37 ± 0.17) and (6.41 ± 0.68) after 1 month and 2 months of culturing, respectively.

Abstract
Introduction
Materials and Methods
    1. Pod materials by artificial self-pollination
    2. Seed media preparation by aseptic condition
    3. Callus induction from protocorm-derivedpseudobulbs explants
    4. Effect of different natural extracts on callusproliferation and PLBs induction from callus culture
    5. Effect of different plant growth regulators and naturalextracts on shoot regeneration and multiplication ofB. auricomum Lindl. from calli-derived PLBs culture
    6. Statistical analysis
Results and Discussion
    1. Morphology of flower and pod materials byartificial self-pollination
    2. Effect of germination on culture medium andcallus induction from subcultured pseudobulbexplants
    3. Effect of different natural extracts on callusproliferation and PLBs induction from callus culture
    4. Effects of different plant growth regulators andnatural extracts on shoot regeneration and multiplicationof B. auricomum Lindl. from calli-derivedPLBs culture
Conclusion
Abbreviations
Conflict of Interests
Literature Cited

• Win Theingi Aung(Doctor’s Degree, Department of Lifestyle Medicine, College of Environmental and Bioresource Sciences, Jeonbuk National University, Iksan 54596, Korea)
• Keuk Soo Bang(Professor, Department of Oriental Medicine Resources, College of Environmental and Bioresource Sciences, Jeonbuk National University, Iksan 54596, Korea) Corresponding author
• Seo A Yoon(Doctor’s Degree, Institute of Life Science and Natural Resources, Wonkwang University, Iksan 54538, Korea)
• Baul Ko(Post-Doctoral Researcher, Urban Agriculture Division. National Institute of Horticultural & Herbal Science, RDA, Wanju 55365, Korea)
• Jong Hyang Bae(Professor, Department of Horticulture Industry, Wonkwang University, Iksan 54538, Korea)