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Comparing the Expressions of Genes Related to Defense Responses by Infection of Phakopsora euvitis in Ampelopsis Species KCI 등재
포도나무 녹병균(Phakopsora euvitis) 감염에 따른 개머루(Ampelopsis sp.) 방어반응 유전자의 발현 비교
- 언어ENG
- URLhttps://db.koreascholar.com/Article/Detail/415211
pp.157-163
Grapevine leaf rust (GLR) caused by Phakopsora euvitis results in the reduction of fruit quality and yield loss in grape production. The purpose of this study was to investigate the expression of genes related with defense responses in the grapevines infected with rust pathogens. In two genotypes of Ampelopsis species inoculated with P. euvitis, the real-time PCR with RNAs was performed to investigate transcripts levels of nine defense-related genes, including pathogenesis-related 1 (PR1), β-1,3glutannase (Glu), chitinase (Chi), superoxide dismutases (SOD), glutathione peroxidase (GPX), phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), stilbene synthase 1 (STS1), and resveratrol O-methyltransferase (ROMT). All tested genes were upregulated in YG11030 as well as YG10075, while mostly the expression of genes was higher in YG10075 than that in YG11030. Glu and STS showed significantly high expression in YG10075 than in YG11030. Expression of ROMT was upregulated in YG11030 and downregulated in YG10075 at 24 hours after inoculation. The differential expression pattern of the tested genes seems to be related with the defense responses, considered to be involved in plant-resistant responses against the infection by P. euvitis, and further studies on resistant responses based on the expression of genes would provide valuable information in breeding grapes resistant to diseases.
Introduction
Materials and Method
Plant materials and pathogens
Investigation of Disease Incidence in the Vineyard
Evaluation of disease occurrence in vitro and in vivo
Inoculation of pathogen
RNA isolation and real-time polymerase chain reaction(PCR) analysis
Results
Assessments of disease incidence in the field
In vitro and In vivo evaluation of disease occurrence
Expression of Pathogenesis related genes
Discussion
적 요
ACKNOWLEDGMENTS
REFERENCES
