한국초지조사료학회지 (JKSGFS) Vol. 42 No. 2 (p.114-119)

Phosphorus Significance in Alleviating Oxidative Stress Induced by Drought in Kentucky Bluegrass (Poa pratensis L.)

키워드 :
Antioxidant enzymes,Drought,Oxidative stress,Potassium phosphate,Potassium phosphonate

목차

ABSTRACT
Ⅰ. INTRODUCTION
Ⅱ. MATERIALS AND METHODS
   1. Plant culture and treatments
   2. Osmotic potential, chlorophyll and carotenoid content
   3. Determination of O2•−, H2O2 and lipid peroxidation
   4. Measurement of antioxidant enzymes activities
   5. Statistical analysis
Ⅲ. RESULTS
   1. Osmotic potential, total chlorophyll and carotenoidconcentration
   2. O2•− and H2O2 content and lipid peroxidation
   3. Activities of antioxidant enzymes
Ⅳ. DISCUSSION
Ⅴ. Acknowledgments
Ⅵ. REFERENCES

초록

The objective of this study was to determine phosphorus effects on drought stress-induced oxidative stress in Kentucky bluegrass. Drought stress was induced by reducing of water to plants in pots. Two types of phosphorus were applied as potassium phosphate (P) or potassium phosphonate (PA). Application of phosphorus was efficient to ameliorate the adverse effects of drought. Osmotic potential, total chlorophyll and carotenoid content were significantly decreased by drought stress, but was relieved by P or PA application. Superoxide (O2 •−) concentration was significantly increased more than 14-fold under drought-stressed plants, was accompanied with increase of hydrogen peroxide (H2O2) and lipid peroxidation (MDA). However, malondialdehyde (MDA) was much less in P or PA applied plants under drought stress condition. Activities of catalase (CAT), ascorbate peroxidase (APX) and guaiacol-peroxidase (GPX) were largely increased by drought stress and its increase rate was much higher in P or PA applied plants except APX. These results indicate that drought stress-induced oxidative stress is alleviated by P or PA application due to the increase of activities of antioxidant enzymes.