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김치유래 Lactobacillus plantarum K-21의 DPPH 라디칼 제거활성 증진 및 다양한 항산화 효과 KCI 등재

Enhanced DPPH Radical Scavenging Activity of Lactobacillus plantarum K-21 Isolated from Kimchi and its Various Antioxidant Effects

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한국환경과학회지 (Journal of Environmental Science International)
한국환경과학회 (The Korean Environmental Sciences Society)
초록

Lactic Acid Bacteria (LAB) are among the representative probiotics that have been used for a long time in fermented food. Although there are many studies on detecting the radical scavenging activity of LAB, few studies have been conducted on the environmental factors that improve scavenging activity. This study investigated the environmental factors affecting the DPPH radical scavenging and various antioxidant activities of Kimchi-derived Lactobacillus plantarum K-21 with antihypertensive and radical scavenging activities. The optimal conditions for scavenging DPPH radicals were glucose 2%, bactopeptone 0.5%, Tween 80 0.05%, L-cysteine 0.05%, and an initial pH 6.5 at 35℃. Under optimal conditions, the DPPH radical scavenging activity was 94.8±2.2%, which was 1.5 times higher than that of the basic medium. In addition, L. plantarum K-21 had other antioxidant activities; ABTS radical scavenging (93.6±1.5%), hydroxyl radical scavenging (8.5±0.9%), metal chelating (65.9±0.5%), NO scavenging (53.1±19%), SOD-like (25.1±1.5%), and reducing power (11.7±1.4%) activities were detected. Therefore, L. plantarum K-21 may act not only as a starter for lactic acid-fermented foods with improved functionality but also as a drug for various diseases caused by oxidative stress.

목차
Abstract
1. 서 론
2. 재료 및 방법
    2.1. 사용 균주 및 배양조건
    2.2. 실험균주의 DPPH 라디칼 제거능에 대한 환경요인의 영향
    2.3. 라디칼 제거 활성 및 항산화 활성 측정법
3. 결과 및 고찰
    3.1. DPPH 라디칼 제거능을 위한 환경요인 최적화
    3.2. 최적조건에서 시간에 따른 DPPH 라디칼 제거활성
    3.3. 실험균주의 다양한 라디칼 제거 및 항산화 활성
4. 결 론
REFERENCES
저자
  • 김예린(부산대학교 생명환경화학과 및 생명산업융합연구원) | Yerin Kim (Department of Life Science and Environmental Biochemistry, Life and Industry Convergence Institute, Pusan National University, Miryang 50463, Korea)
  • 김예담(부산대학교 생명환경화학과 및 생명산업융합연구원) | Yedam Kim (Department of Life Science and Environmental Biochemistry, Life and Industry Convergence Institute, Pusan National University, Miryang 50463, Korea)
  • 전채민(부산대학교 생명환경화학과 및 생명산업융합연구원) | Chae-Min Jeon (Department of Life Science and Environmental Biochemistry, Life and Industry Convergence Institute, Pusan National University, Miryang 50463, Korea)
  • 박규림(부산대학교 생명환경화학과 및 생명산업융합연구원) | Gyulim Park (Department of Life Science and Environmental Biochemistry, Life and Industry Convergence Institute, Pusan National University, Miryang 50463, Korea)
  • 이오미(농림축산검역본부 조류세균과) | O-Mi Lee (Avian Disease Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea)
  • 손홍주(부산대학교 생명환경화학과 및 생명산업융합연구원) | Hong-Joo Son (Department of Life Science and Environmental Biochemistry, Life and Industry Convergence Institute, Pusan National University, Miryang 50463, Korea) Corresponding author