This study aimed to investigate the biological activities, including polyphenolic content, flavonoid content, and antioxidant activities, of various cultivars of Korean perilla leaves. The results indicated that among nine cultivars (Namcheon dlggae, Saedora, Nulbora, Donggel 1, Donggell 2, Soim, Sangyeop, Somirang, and Saebom) of perilla leaves, the total polyphenolic content (gallic acid equivalent mg/g, GAE) was the highest in “Nulbora,” while it was lowest in Namcheon dlggae. Moreover, flavonoid content in the extracts of nine cultivars leaves was in the range of 132.93~268.50 mg catechin equivalent/g sample. The antioxidant effects of the perilla leaves were determined using two different in vitro bioassays measuring DPPH and ABTS radical-scavenging activities. The results revealed that antioxidant activity was also higher in “Nulbora” compared with other cultivars. Xanthin-oxidase-inhibition activity ranged from 65.65% to 80.58%, with “Nulbora” exhibiting the highest activity, although the difference with other cultivars was not significant. “Nulbora” extracts reduced the expression of pro-inflammatory genes and several cytokines, including IL-6 activation induced by LPS in macrophages in the range of 100–50 μg/mL. These results suggest that extracts from perilla leaves can be used as bioactive and functional materials that could be important in industrial applications in the future.

Abstract
서 론
연구 내용 및 방법
    1. 재료 및 시약
    2. 추출물 제조
    3. 클로로필 a,b 함량 분석
    4. 총 폴리페놀 및 플라보노이드 함량 분석
    5. ABTS 및 DPPH 라디칼 소거능 측정
    6. Xanthin oxidase 저해활성 측정
    7. 세포배양 및 세포독성 평가
    8. 염증성 사이토카인(IL-6) 생성 억제 효능 평가
    9. 통계분석
결과 및 고찰
    1. 품종별 잎들깨 추출 수율 및 클로로필 a,b 함량
    2. 품종별 잎들깨의 ABTS 및 DPPH 라디칼 소거능
    3. 품종별 잎들깨의 총 폴리페놀 및 총 플라보노이드 함량
    4. 품종별 잎들깨 추출물의 xanthin oxidase inhibition 활성
    5. 품종별 잎들깨 추출물의 항염활성 평가
요약 및 결론
감사의 글
References

• 김현영(농촌진흥청 국립식량과학원 작물기초기반과 농업연구사) | Hyun Young Kim (Associate Researcher, Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Korea) Corresponding Author
• 이한결(전북대학교 자연과학대학 생물학과 박사과정생) | HanGyeol Lee (Doctor’s Student, Division of Life Sciences, Jeonbuk National University, Jeonju 54896, Korea)
• 서혜영(농촌진흥청 국립식량과학원 작물기초기반과 연구원) | Hye Young Seo (Researcher, Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Korea)
• 서우덕(농촌진흥청 국립식량과학원 작물기초기반과 농업연구관) | Woo Duck Seo (Research Officer, Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Korea)
• 이미자(농촌진흥청 국립식량과학원 작물기초기반과 농업연구관) | Mi Ja Lee (Research Officer, Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Korea)
• 송승엽(농촌진흥청 국립식량과학원 작물기초기반과 농업연구사) | Seung-Yeob Song (Associate Researcher, Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Korea)
• 김정인(농촌진흥청 국립식량과학원 남부작물부 밭작물개발과 농업연구사) | Jung In Kim (Associate Researcher, Dept. of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, Miryang 50424, Korea)
• 최준열(농촌진흥청 국립식량과학원 작물기초기반과 농업연구관) | June-Yeol Choi (Research Officer, Division of Crop Foundation, National Institute of Crop Science, Rural Development Administration, Wanju 55365, Korea)