The research was conducted at a degraded steppe in Saibei Adiministrative Region of ZhangJiakou in Hebei province. Four patches dominated by fringed sagebrush (Artemisia frigida), narrowleaf stellera (Stellera chamaejasme), shining speargrass (Achnatherum splendens), white swordflag (Iris lactea), respectively, were sampled. Phosphorus (P) was applied at four levels (0, 5, 10 and 15㎎ ㎏?¹ soil) in each patch. The mycorrhizal infection rate in Stellera chamaejasme species and Achnatherum splendens species was low, differed from other two patches of Artemisia frigid and Iris lactea.
The root crown of forage legume is an important organ which connects underground root system and aboveground branches. And its morphological characteristic is closely related to yield of grass, overwintering ability, regeneration in spring and sustainable productivity of Medicago sativa L. Therefore, in order to understand the morphological mechanism on cold resistance of the root of Melilotoides ruthenica (L.) Sojak t㏊t is a relative species of M sativa, the root morphology of different growth years' M ruthenica had been observed and analyzed. It aims to provide theoretical basis to M. sativa genetic improvement.
Biolog ECO plates (31 different carbon sources, Biolog Inc., Hayward, USA) were used to discriminate between rhizosphere soil samples from 4- and 9-year alfalfa stands each with three replication. The growth curves for different groups of carbon sources were nearly sigmoidal, but the maximum rate of utilization was faster for amino acids, carbohydrates and polymers t㏊n for amides, miscellaneous and carboxylic acids, and carbon sources utilization efficiency were all higher in 9-year t㏊n in 4-year alfalfa stand.
The temporal variations of soil CO₂ efflux (Rs) under sown alfalfa pasture and rangeland in the Loess Plateau was studied. Results showed t㏊t diurnal changes of soil CO₂ efflux were highly associated with changes of soil temperature, with tendency t㏊t the minimum values occurred between 0100 to 0600 (Beijing Standard Time, BST), maximum value between 1200 and 1600. Soil temperature at 5㎝ depth gave the highest correlation coefficient and account for 72.2% and 89.5% of diurnal variation in soil CO₂ effluxes at rangeland and alfalfa pasture, respectively. Annual CO₂ emissions from alfalfa pasture were 488.5 g C/㎡/yr, which was higher t㏊n t㏊t from rangeland.
In this paper, the effect of different land-use types on soil organic carbon (SOC) and Particulate Organic Carbon(POC) was studied by analyzing the soils in the meadow and conversing land of grassland to cropland in ecotones of agriculture and animal husbandry. It is shown t㏊t, compared with meadow, rotations of P/O and P/Cwill reduce SOC in 0-30㎝ soil layers by 47.54%-65.11% and 34.16-64.26% and POC by 16.90%-24.48% and 11.94%-20.29%, respectively. And SOC in meadow is more t㏊n in conversing land of grassland to cropland (P<0.05). Conversing grassland to cropland in ecotones of agriculture and animal husbandry will decrease the contents of SOC and POC.
In this paper, by investigating soil, field vegetation, and surface wind erosion status, combining in2 door analysis, the effect of vegetation recovery on sandy degraded grassland in Yanchi is studied. The results show t㏊t : after the process of protective growth, grazing rest and reseeding, the plant species and vegetation coverage are increased, and biomasses are enhanced about 66. 3214, at the same time, the effect of the measure of combining protective growth, grazing rest and reseeding is better t㏊n t㏊t of single protective growth and grazing rest, the formers biomass is 1. 54 times more t㏊n the latter. With the fixing of drifting - sand and the recovering of vegetation in demonstration area, the content s of physical clay minerals, organic matter, total N, available P and available K are all increased compared with those on drifting - sand. The thickness of wind erosion in drifting - sand control area is reduced, and the thickness is different in different vegetation recovering area, in which Artemisia sphaerocephala planting area suffers slight wind erosion; Caragana korshinskii forest present s slightly accumulating status, the accumulating thickness in Tamarix and Salix psammophila forest is the most.
In order to easily and rapidly establish low-cost grazing grasslands using centipedegrass (Eremochloa ophiuroides (Munro) Hack.) (CG) on abandoned cultivated land, the influences of slope direction and angle on the covering speed of CG were investigated. We established 61 quadrats at varying slope directions and angles, and the planting of CG was performed in 2003. The covering speed of CG was investigated from 2003 to 2008, and the years of point of inflection (YPI) was calculated. The most rapid YPI was 1.0-1.5 years in the quadrats in the S5°W direction with 0 degree slopes, while the slowest YPI was 3.0-3.5 years in the quadrats in the northern direction with 10-20 degree slopes. Therefore, grassland renovation using low quantities of CG seeds, which are sold at a high price in Japan, is favorable on land in the southern direction and with a lower slope.
To investigate the effect of S-availability on N and S assimilations, Enzymatic activity of nitrate reductase (NR), glutamine synthetase (GS), ATP sulfurylase (ATPS) and O-acetylserine(thiol)lyase (OASTL) was measured, and also followed by their activity staining in forage rape (Brassica napus L.) fed with three different SO₄²? levels (0, 0.1 and 2.0 mM) for 25 hours. NR activity decreased as S-availability decreased, especially in young and middle leaves. S-limited conditions (0 and 0.1 mM sot) resulted in a reduction of GS activity. The activity of ATPS tended to increase with decreasing the exogenous SO₄²? supplying level. The activity of OASTL also increased as S-availability decreased for all leaf tissues, while tended to decrease in root. The results indicated t㏊t N assimilation was restricted by the limited S-nutrition especially in the developing leaf tissues, but S assimilation was stimulated except for OASTL activity in the roots.
Field research was undertaken to determine how barley + legume mixtures influence nitrogen fixation and N transfer to barley. Barley + legume (main plot) and 4 different sowing rate was the experiment design. Isotope dilution was used to estimate N₂ fixation and subsequent transfer to barley. Botanical composition of legume was from 1.6% to 13.9% when harvest. Legume N derived from atmosphere ranged from minus value to 58percentage in whole plant N. N transfer amount to barley varied from 12.11㎏ to 7㎏ by different method.
Soil temperature is an important factor influencing crop growth. Within limits, a higher soil temperature will promote crop growth, especially in cool climate regions. Compost application can increase soil temperature, however the mechanism of increasing soil temperature remains unclear. We conducted a pot experiment to examine the effects of compost application on soil temperature and evaporation in a bare Andisol. Pots with compost (16㎏ m?²) had a higher soil temperature and less evaporation than pots without compost. The decrease in evaporation and the increase in soil temperature by compost application were significantly correlated. We conclude that compost application increases soil temperature by decreasing evaporation from the soil surface.
The experimental work was conducted to investigate the effects of the application level of composted cattle manure (CCM) on forage productivity using rye-whole crop rice (WCR) double cropping system for 3 years. Main plot was consisted of application level of CCM such as 150%, 200% and control plot. The total DMY of of rye-WCR in treatment of CCM 150% significantly decreased as compared with that of treatment of CF and CCM 200%. However, NDF, ADF and CP content of rye and WCR were hardly influenced by CCM application.
The experimental work was conducted to investigate the effects of the application level of composted cattle manure (CCM) on forage productivity using Italian ryegrass-WCR double cropping system for 3 years (2006~2008). Main plot was consisted of application level of CCM such as 150%, 200% and control plot. The total day matter yield (DMY) of Italian ryegrass and WCR in treatment of CCM 150% and CCM 200% significantly decreased as compared with that of treatment of chemical fertilizer (CF). However, NDF, AND and CP content of Italian rye grass and WCR were hardly influenced by CCM application.
The experimental work was conducted to investigate the effects of the application level of composted cattle manure (CCM) on forage productivity using whole crop barely (WCB)-whole crop rice (WCR) double cropping system for 3 years. Main plot was consisted of application level of CCM such as 150%, 200% and control plot. The total DMY of forages under WCB and WCR in treatment of CF significantly decreased as compared with that of treatment of CCM 150%. However, NDF, ADF and CP content of WCB and WCR were hardly influenced by CCM application.
This study was carried out to investigate the effects of cattle slurry application on the productivity and nutritive values of whole crop rice in paddy land. A field study was conducted at Baeksan-myeon, Kimje, Jeonbuk province in Korea on clay loam soil for 3 years. All plots were arranged in completely randomized design with three replicates. The yield of dry matter (DM) of whole crop rice in treatment of cattle slurry significantly decreased as compared with that of treatment of standard fertilizer (P<0.05). However, nutritive values of whole crop rice were not different significantly between both treatments.
This study was carried out to investigate the effects of cattle slurry application in paddy land cultivated with whole crop rice on the properties of soil and water. A field study was conducted at Baeksan-myeon, Kimje, Jeonbuk province in Korea on clay loam soil for 3 years. All plots were arranged in completely randomized design with three replicates. The contents of organic and inorganic properties in soil samples collected at the end of the experiment were remarkably higher than those at the beginning of the experiment (P<0.05). The concentrations of NO₃-N, NH₃-N and PO₄-P in discharge and leaching water were different significantly between both treatments.
This experiment was conducted to determine the effects of applying level of pig slurry on the characteristics of runoff and leaching water in lysimeter. Treatments were broken down into five treatments such as non-chemical fertilizer, chemical fertilizer (200㎏ N/㏊) and pig slurry (200, 400, 600㎏ N/㏊), and 3-time-repeated randomized complete block design was fulfilled. The concentrations of NO₃-N was raised(p<0.05) in proportion to the applying level of pig slurry. NO₃-N concentration of leaching water collected from soil depth 20㎝ and 40㎝ was intensified as the applying level of pig slurry was higher (p<0.05) in pig slurry 600㎏ N/㏊ application. In conclusion, pig slurry at the volcanic ash soil in Jeju area can replace the chemical fertilizer as it is tested that applying 200㎏ N/㏊ of pig slurry and chemical fertilizer show the same productivity of sorghum×sudangrass hybrid. But more than 200㎏ N/㏊ of pig slurry may not be appropriate because it may contaminate the runoff and leaching water even though it may increase for the forage productivity.
SRAP (Sequence-related amplified polymorphism) and ISSR (Inter simple sequence repeat) molecualr markers were used to evaluate the levels and patterns of genetic diversity among 45 collections of orchardgrass from four continents. Twenty-one primer combinations were used and 480 bands were produced in SRAP, of which 405(84.38%) were polymorphic. On the other hand, twelve primers were used to generate a total of 116 bands in ISSR, of which 116(87.07%) were polymorphic. The coefficient range of genetic similarity was 0.6248-0.9686 and 0.6116-0.9231 respectively. Based on cluster and principal component analysis on the genetic characteristics, all collections could be divided into four groups and five groups in two markers, respectively. According to the analysis of genetic diversity and relationships, the appropriate strategies for collection and conservation of germplasm resources also were discussed and scientific breeding with far genetic relationship materials in orchardgrass were suggested.
Phylogenetic relationships among 53 accessions of D.glomerata collected from 5 continents were investigated using simple sequence repeat (SSR) markers. 15 SSR primer pairs generated a total of 127 alleles, with an average of 8.5 alleles per locus. The average polymorphic rate (P) was 95.21 %. The genetic similarity (GS) among all accessions ranged from 0.43 to 0.94, with an average of 0.63. Analysis of molecular variance (AMOVA) indicated that larger proportions of variability existed within geographical regions (73.75%). High degree of genetic diversity was observed in Asia (P, 90.55%) and Europe (P, 86.61%) groups. Based on the cluster and principal component analysis, 53 accessions could be divided into five groups (GS=0.64) according to the nearest phylogenetic relationship.
Genetic variation within and among 12 populations of whip grass in south China were investigated using inter-simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP). High genetic diversity was found in whipgrass by two molecular makers (PPB=86.21%, I=0.357 based on ISSR; PPB=82.21%, I=0.352 based on SRAP). However, there was relatively low genetic diversity at population levels. A high degree of genetic differentiation among populations was detected based on different measures and different molecular markers. We also found that SRAP markers were more efficient than ISSR markers in this species. Based on these findings, sampling strategy was proposed for successfully utilizing the genetic resource of this species.
The cultivation of oat in China could be traced back to 2500 B.C. while the Qinghai province, which located in the North-East of the Qinghai-Tibetan Plateau, has been the major region for oat production due to its high quality caused by the unique climate say long sun-shine hours and large temperature discrepancy between days and nights that is beneficial to the accumulation of nutrients in oat plant. Oat has been grown in the Qinghai alpine region on the altitude ranging from 1600m to 3600m and regarded as an ideal forage plant due to its resistance to environment. The role of oat drew more attention in 1980s along with the implementing of so called four-matched policy, which compounds establishment of fenced rangeland, animal bars, fodder production base and settled living place for local herdsmen who used to be nomadic around the region. To make a full use of the oat resource in Qinghai province, a great deal research and extension with respect of oat had been engaged in the region. The author overviewed researches accomplished in Qinghai province on oat germplasm collection, breeding of fine oat variety, cultivate techniques, oat base establishment and production and marketing system establishment as well in terms of projects completed.