간행물
한국초지조사료학회 학술대회논문집
- 발행기관 한국초지조사료학회
- 자료유형 학술대회
- 간기 연간
- 수록기간 1998 ~ 2020
- 주제분류 농수해양 > 축산학 농수해양 분류의 다른 간행물
- 십진분류KDC 527DDC 636
권호리스트/논문검색
한국초지조사료학회 2009년도 제3회 한중일 국제 심포지움 (2009년 8월) 122건
61.
2009.08
구독 인증기관·개인회원 무료
39 accessions of sickle alfalfa (Medicago falcata L.) collected from different regions of China were evaluated for their tolerance to drought. Differences were observed among accessions in their reactions to drought and 8 good tolerant accessions at populations level and 18 excellent tolerant accessions at individual level were identified based on drought response index (DRI).
62.
2009.08
구독 인증기관·개인회원 무료
Sorghum-Sudan grass which is an annual forage grass in Gramineae have been bred for biomass promotion. The recombinant inbred line (RIL) populations were derived from the F2:3 genetic mapping population of Sorghum 314A×zongke Sudan grass. The breeding selection based on agronomic characters and DNA molecular marker statistical analysis was carried among the progenies of the RIL populations, and 9 recombinant breeding progenies were determined as heterobeltiosis with high yield. According to DNA diversity analysis of simple sequence of repeats(SSR) marker, the genetic varieties were abundant among the families, and the recombinant breeds of high yield were distributed among the different RIL populations.2 family lines, which have similar agronomic characters and yield, and whose genetic distance nearly equal to zero, showed difference on arid tolerance. 4 breeding lines were selected on agronomic characters and resistance in the field trials, and 2 breed lines were qualified for the regional testing.
63.
2009.08
구독 인증기관·개인회원 무료
Thee kinds of explants, young spikes, immature and mature embryos, was compared in callus induction, embryogenic callus formation, differentiation and plant regeneration of Russian wildrye. Callus induction efficient of young spikes is highest. The imature embryos formed more somatic embryogenesis, and with highest frequency of embryogenic callus.
64.
2009.08
구독 인증기관·개인회원 무료
In this study, GSTs gene homology fragment from Mongolian wheatgrass(Agropyron mongolicum Keng) was isolated using homology-based method. one partials gene sequences have been obtained by reverse transcription polymerase chain reaction (RT-PCR). Sequence analysis using Software of DNAman and DNAuser etc, and showed that the cDNA sequences was 344 base pairs, encoding 62 amino acids. The partial gene had C-terminal conserved domains of substrate binding pocket (H-site) of GSTs superfamily. Homology comparison with GSTs gene amino acid sequences in other plants showed that it was 91% identical to 19E50 of wheat, 90% to pk0078 and 88% to gstf6. It was named as MwGSTs.
65.
2009.08
구독 인증기관·개인회원 무료
The ploid level of 46 germplasms of Elytrigia Desv. were observed and determined with the microscope, which are from 21 countries and areas that United States of America offered. The results showed these accessions were grouped into three distinct ploid levels including diploidy, tetraploidy and hexaploidy in E. intermedia, E. repens, E. elongata, E. pontica, E. caespitosa, E. juncea, E. alatavica. There were 24 diploid accessions, 1 tetraploidy and 1 hexaploidy in E. intermedia; 4 diploid accessions and 3 tetraploidy in E. repens, 6 texaploidy and 1 hexaploidy in E. elongata, 1 diploidy and 2 tetraploidy in E. pontica, all of E. caespitosa, E. juncea and E. alatavica were diploidy.
66.
2009.08
구독 인증기관·개인회원 무료
Sang-Hoon Lee, Ki Won Lee, Ki-Yong Kim, Hee Chung Ji, Sung Seo, Hyung Soo Park, Young Chul Lim, Gi Jun Choi
In the present study, we have used an annealing-control-primer (ACP)-based differentially display RT-PCR method to identify salt-stress-induced differentially expressed genes (DEGs) in barley leaves. Using 120 ACPs, a total of 11 up-regulated genes were identified and sequenced. Temporal expression patterns of some up-regulated DEGs in response to salt stress were further analyzed by Northern blot analysis. The possible roles of these identified genes are discussed within the context of their putative role in response to salt stress. Thus, the identification of some novel genes-such as SnRK1-type protein kinase; 17 kDa, class I, small heat shock protein; and RNase S-like protein precursor genes-may offer a new avenue for better understanding the salt stress response in plants, knowledge which might be helpful for developing future strategies.
67.
2009.08
구독 인증기관·개인회원 무료
Environmental stress is a growing problem for the productivity of forage crops. Reactive oxygen species (ROS) formation due to several abiotic stresses is a fundamental process that interrupts several physiological processes and causes a significant reduction on growth and yield of many forage crops. Molecular breeding such as genetic transformation has become a popular biotechnological tool for improving forage quality as well as improves tolerance to various abiotic stresses. As a first step of genetic transformation in tall fescue, we established an efficient Agrobacterium-mediated genetic transformation protocol using mature seed-derived embryogenic callus. After optimization of the transformation system, several genes of interest have been used to generate abiotic stress tolerant forage grasses. We generated transgenic tall fescue plants expressing NDPK genes under the control of the oxidative stress-inducible SWPA2 promoter. Transgenic plants showed enhanced tolerance to several abiotic stresses. Results in the current study, suggest that NDPK mediated multiple stress tolerance by increasing the expression of genes involved in antioxidant and protective functions, possibly through activation of an MAPK cascade.
68.
2009.08
구독 인증기관·개인회원 무료
Sang-Hoon Lee, Ki Won Lee, Ki-Yong Kim, Hee Chung Ji, Sei Hyung Yoon, Jong Geun Kim, Min Woong Jung, Gi Jun Choi
Perennial ryegrass (Lolium perenne L.) is one of the most important grass species in the world's temperate zones. It is used as high-quality forage in pastures and for recreational use as turf in golf courses, lawns and parks. Genetic improvement of perennial ryegrass is difficult due to its self-incompatibility. Consequently, progress by conventional breeding can be slow. Genetic transformation is an alternative that permits direct introduction of useful genes into a plant's genome and is becoming a powerful tool to complement conventional breeding. To improve environmental stress tolerance and quality of perennial ryegrass by introducing better and useful genes into the genome, we have developed a rapid and efficient transformation system using Agrobacterium-mediated gene transfer system.
69.
2009.08
구독 인증기관·개인회원 무료
Ki-Won Lee, Gi Jun Choi, Ki-Yong Kim, Ki-Yong Kim, Hee Chung Ji, Joung Kyong Lee, Hyung Soo Park, Young Chul Lim, Sang-Hoon Lee
In the present study, genotypic variation of Agrobacterium-mediated transformation of Korean Italian ryegrass has been evaluated. Mature seed-derived calli of a total of seven cultivars were infected and co-cultured with Agrobacterium tumefaciens carrying the binary vector pCAMBIA1301, which contains a reporter gene (gus) and a plant selectable marker gene conferring resistance to hygromycin (hpt) in the T-DNA region. The effects of several factors such as callus type and callus age on transformation frequency and the expression of the GUS gene were investigated. The highest transformation frequency (6.7%) was obtained with the Hwasan 101 cultivar when 9-week-old calli (type-I) were inoculated with Agrobacterium. The overall transformation rates of the examined cultivars ranged from 0.4% to 6.7%. GUS histochemical assays, PCR, and southern analysis of transgenic plants demonstrated that transgenes were successfully integrated into the genome of Italian ryegrass. Thus, optimization of transformation frequency and selection of a suitable cultivar of Italian ryegrass may improve molecular breeding of this species.
70.
2009.08
구독 인증기관·개인회원 무료
Ki-Won Lee, Gi Jun Choi, Ki-Yong Kim, Sei Hyung Yoon, Joung Kyong Lee, Hyung Soo Park, Young Chul Lim, Sang-Hoon Lee
In the present study, we have developed a high-frequency plant regeneration system for Italian ryegrass via callus culture using mature seeds as explants. Optimal embryogenic callus induction was found to occur in MS medium containing 5 ㎎ 1?¹ 2,4-D, 0.5 ㎎ 1?¹ BA, 500 ㎎ 1?¹ L-proline, 1 g 1?¹ casein hydrolysate, 30 g 1?¹ sucrose, 7 ㎎ 1?¹ AgNO₃, 2 ㎎ 1?¹ CuSO₄ and solidified with 3 g 1?¹ Gelrite. The highest regeneration rate was obtained in MS medium containing 1 ㎎ 1?¹ 2,4-D, 5 ㎎ 1?¹ BA, 500 ㎎ 1?¹ L-proline, 1 g 1?¹ casein hydrolysate, 1 ㎎ 1?¹ thiamine-HCl, 30 g 1?¹ sucrose, 7 ㎎ 1?¹ AgNO₃, 2 ㎎ 1?¹ CuSO₄ and solidified with 3 g 1?¹ Gelrite. By using the most effective treatment determined for each parameter, the highest rates of embryogenic callus formation (48.9%) and regeneration (47.6%) were obtained with the Hwasan 101 cultivar. The overall plant regeneration rates of the examined cultivars ranged from 7.5% to 23.2%. Thus, optimization of regeneration frequency using mature seeds as explant material may offer a simple and efficient protocol for Italian ryegrass that may improve molecular breeding of this species.
71.
2009.08
구독 인증기관·개인회원 무료
Antibiotics marker-free with herbicide-resistant tall fescue plants were produced through Agrobacterium: mediated transformation system. Embryogenic calli derived calli were infected with Agrobacterium tumefaciens strain EHA105 harboring the pCAMBIA3300 vector containing the bar and the CP4-EPSPS genes. The PPT-resistant calli and plants were selected with 10 ㎎/L PPT, respectively. Soil-grown plants were obtained about 14-16 weeks after Agrobacterium-mediated transformation. Genetic transformation of the regenerated plants growing under selection was demonstrated by PCR, and Southern blot analysis revealed that one copies of the transgene were integrated into the plant genome of each transgenic plant. Expression of the bar and CP4-EPSPS genes in transgenic plants was confirmed by Northern blot analysis and CP4-EPSPS genes in transgenic plant ELISA uses antibody protein by ELISA Assays. Transgenic plants sprayed of two herbicides with glufosinate ammonium or glyphosate remained green and healthy. We therefore report here a successful and reliable Agrobacterium-mediated transformation of two herbicide-resistances and this method may be useful for routine transformation and has the potential to develop new varieties of tall fescue with several important genes.
72.
2009.08
구독 인증기관·개인회원 무료
C. -H. Park, Y. -G. Kim, K. -H. Kim, I. Alam, C. -H. Joen, H. -J. Lee, K. -W. Lee, S. A. Sharmin, J. -K. Yang, M. -S. Choi, B. -H. Lee
73.
2009.08
구독 인증기관·개인회원 무료
74.
2009.08
구독 인증기관·개인회원 무료
Yong-Goo Kim, Choong-Hoon Park, Kyung-Hee Kim, Hyo-Jin Lee, Chan-Ho Jeon, Iftekhar Alam, Shamima Akhtar Sharmin, Byung-Hyun Lee
75.
2009.08
구독 인증기관·개인회원 무료
Kyung-Hee Kim, Choong-Hoon Park, Iftekhar Alam, Yong-Goo Kim, Hyo-Jin Lee, Ki-Won Lee, Sang-Hoon Lee, Sang-Soo Kwak, Sang Yeol Lee, Byung-Hyun Lee
76.
2009.08
구독 인증기관·개인회원 무료
Chan-Ho Jeon, Choong-Hoon Park, Iftekhar Alam, Hyo-Jin Lee, Yong-Goo Kim, Kyung-Hee Kim, Shamima Akhtar Sharmin, Byung Hyun Lee
77.
2009.08
구독 인증기관·개인회원 무료
78.
2009.08
구독 인증기관·개인회원 무료
79.
2009.08
구독 인증기관·개인회원 무료
Whipgrass [Hemarthriacompressa(Linn.f.)R.Br.] has several outstanding characteristics that make it attractive in livestock production systems. These include superior cool-season growth compared to other C₄ grasses, persistence under grazing on poorly drained soils, and relatively high herbage digestibility that declines at a slower rate with increasing maturity than for most C₄ grasses. More than 50 years hard work, a whipgrass breeding group in Sichuan Agricultural University (SAU) collected 84 accessions from south china. Through germplasm evaluation and breeding, 3 varieties, Chong gao, Guang yi and Ya an, were released by the end of 2009. All of them are very popular in south china from 1986. In the future, the team approach emphasizes plant improvement in seedling vigor and establishment, seed production, cold tolerance, photoperiod response, seasonal distribution of forage production, forage quality, insect and disease resistance.
80.
2009.08
구독 인증기관·개인회원 무료
Fifty-four biological characters were used for quantitative classification of sorghum and sudangrass and their relationship. Discriminant analysis of all 240 individuals of 48 accessions suggested that 100% of originally grouped cases were correctly classified. Analysis of variance results showed that the differences among 48 accessions in characters were significant at the 0.01 level. Cluster analysis showed that the 48 accessions were obviously divided into two clusters by a Euclidean distance of 17.46 as the classification standards, i.e. the genus maize and genus sorghum. The 48 accessions were divided into four clusters by a Euclidean distance of 9.86, i.e. Sorghum-Sudangrass, Sorghum related species (S. propinquum and S. halepense), Zea mexicana and Z. mays. All accessions were divided into five clusters by a Euclidean distance of 8.91, namely group Ⅰ (Sudangrass-Chinese sorghum), group Ⅱ (Non-Chinese sorghum), group Ⅲ (Sorghum related species), group Ⅳ (Zea mexicana), and group Ⅴ (Z mays).
