간행물
대한구강악안면병리학회지 KCI 등재 The Korean Journal of Oral and Maxillofacial Pathology
- 발행기관 대한구강악안면병리학회
- 자료유형 학술지
- 간기 격월간
- ISSN 1225-1577 (Print)2384-0900 (Online)
- 수록기간 1977 ~ 2024
- 주제분류 의약학 > 치의학 의약학 분류의 다른 간행물
- 십진분류KDC 515DDC 610
권호리스트/논문검색
제31권 제2호 (2007년 4월) 29건
21.
2007.04
구독 인증기관·개인회원 무료
Cholesterol granuloma(CG) occurs frequently in association with chronic middle ear diseases. pa rti cul arly dis eases in the mastoid antrum a nd ai r cells of the tempora l bone. and much less frequent ly in paranasal sinuses, It occurs f1'eq uentJy seconda1'Y to massive hemor1'hage of oral and pal'aol'aJ cysts, Howeve1'. It has nevel' been I'eported to OCCUI' solely wi thout any association of pl'eexisting lesion in the mandibJe We expel'ienced development of unusuaJ cholestel'ol granuloma in the mandible, Seventy year old female pl'e sented diffuse ha1'd swelling on the left mandibular a rea with Iymphadenopathy in the left cer vical Iymph node Radi og1'aphic exarnination showed a well circumsc ribed multi locular radiolucency resembling soap bubble appeara nce with tooth di s placement and root resorption‘ leading to the radiogrphic imp1'ession of dentigerous cyst 0 1' 。dontogenic cys t or amelobJas toma, CT showed bucco-lingua lly undul ating expansi le lesion with co rticated ma l'gin from the left posterior mandibular bocly to the anterior ramus. including #46, #47 ancl #43. and the mass containing in the lesion showed s lightly lower a ttenuation than muscJe leading to the impression of ameloblastoma, The mass aftel' surgical excis ion composed of 3 sac like structures, measuring 4,1 cm, 1, 3cm ancl 1.4cm in diame ter respectively, One sac was t ightl y a ttached to the #46. l'eRembling dentigerous cyst, Mi croscopic examination showed a large numbel' of c h이 es te rol clefts in association with hemol'rhage, hemosiderin pigments and fO l'eign body giant cells, There was no evidence of cyst 0 1' other lesions, CG should be taken into diffe l'enti a l diagnosis in addition to odontogenic cysts and tumors when radiographica lly well ci rcumscribed multilocuJal' radiolucent lesion occurs
22.
2007.04
구독 인증기관·개인회원 무료
The nitric oxide(NO) is a major factor contri buting to t he loss of neurons in ischemic st roke. demyelina t ing diseases, and other neurodegenerative di sorders . But it is known that NO is not function ing as a direct neurotoxin. NO combined with superoxide(02-) by the diffusion-contl'O ll ed reaction, formed a peroxin it ri te anion (ONOO-)‘ which this s pecies has been shown to contribute to oxidative s igna ling and damage. ONOO stimuJates apoptosis in many cell types. whether ONOO acts direct ly as an ox idant 0 1' the induction of apoptosis is because of the radicals derived from ONOO- decompositi on . But. the mecha ni sm by which ONOO- induces apoptosis is un clear although subsequent forrnation 0 1' reactive oxygen s pecies(ROS) has been suggested in a few reports The aim of this study is to investigate the a nti -apoptotic pathway by inhibi tion 0 1' ONOO synthesis t hrough scavenging of ROS us ing s pecific wavelength 0 1' light irradiation . The present study investi gated the a nti -apop totic effect of the specific wavelength 0 1' irradi ation in Sodium Nitroprusside(SNP) t reated SJ-I-SY5Y ceJls, by MTT, DNA fragmentation, and flow cytometric assay and th rough western blot and caspase-3, -9 activity assay for confirmation of caspase pathway. Also. NO reJease and ROS leveJ was measured in order to observe the changes of NO involved in radical by Griess reaction analysis and DCF'-DH. Results showed that the cell viability were r educed by about 50% of control group by SNP treatment, but re covered to about 80% by 590nm irradiation . The apoptotic cells were observed by flow cytomet ry and DNA fra g mentation assay in SNP-treated group‘ but 590nm irradiation led apoptotic feature to be reduced . Released NO a nd ROS level were increased after SNP treatment but ROS level was dec reased in 590nm irradi at ion - treated group, in spite of high NO concentration fo llowing SNP treatment Also. SNP t reatment led cytochrom C release but 590nm irraidiation inhibit it, hence the expression 0 1' caspase-3 and -9 was dec reased sign ificantly‘ These results showed that 590nm irradiation protect neuronal death thl'Ough bl ocking of NO-induced mi tochondri al apoptotic pathway. Also, it suggests that specific wavelength of irradi ation was used for prevent ion from neurodegenerative disordcr progression
23.
2007.04
구독 인증기관·개인회원 무료
The purposes of this study were to estimate Brdu positive and apoptotic cells dis tribution in co ndyl ar art icular and proliferative layer. and hi stopathological evaluat ion during one sided mas t icatory condition. 15 of 30 adults Sprague Dawley male rats were experimental group, and 15 rats were control group. Experime nta l group were gen t ly extracted on all lower and upper molar teeth to make unilateral mastication. Experimental gl'Oup were divided into two group as extracted side and nonextracted side condyle. Diluted bromodeoxyuridi ne(Brdu) solution(5mg/Kg) was injected in peri toneum before sacrifice at inte rval 1 week, 2 week, 4 week Streptoavidi n-Biotin method for Brdu was used to evaluate cellular proliferat ive activity, and fluorescent TUNEL method was used to estimate the a poptotic activity. The resul ts of this experiment were recorded about anterior and posteri or condyle sepa rately On anterior condyle, contl'Ol group was sustained increased proliferative activity th roughout experiment. whi le cel lu lar proliferative activity of extracted and nonextracted s ide condyle showed more decreased than control grou p ‘ On posteri or portion of condyle‘ control group and nonextracted group showed dramatically decreased cellular pr。 liferat ive activity during all expel‘ imental period. On anterior portion of condyle, control grollp s howed decreased a poptotic activity with time passed. bllt expel'imental gl'oup(both ext l'acted and nonextracted) exhibi ted incrcased a poptotic activity. Es pecia lly extracted grollp showed prominent increased apoptotic activity. On posteri or portion of condyle. extracted group showed dec reased apoptotic activity wi th time progress. but co ntrol and nonextracted group exhibi ted increased apoptotic activity with time progress. Conclllsively. antel'ior portion of condyle on ex t racted s ide expressed hypoplasia by low cellllJar prol iferative activity and increased apoptotic activity. and poste ri or portion on extracted side showed condylar surface hyperplasia by continious proliferative cell ular activity and low a poptotic activity. a nd t hllS unmasticatol'y condyle had anLeroposLel'iorly shorter mo rph이 ogy and verti ca11y longer morphology than masticatory and nOl'mal functioning condyle
24.
2007.04
구독 인증기관·개인회원 무료
Heat shock p1'otein (Hsp) 40 acts as a co-cha perone of Hsp70 by fac ilitating the ATPase activity of Hsp70 as well as promoting the protein fo lding and rena turation by Hsp70. In the present study. Hsp40 gene was fu sed with a gene f’ragment encoding the HJV-l TAT protein transduction domain(YGRKKRRQRRR) in a bacterial exp1' ession vector pTAT-HA to produce the TAT-fused Hsp40(TAT- Hsp40). Purified TAT-Hsp40 was effectively t 1'ansduced into the HEK 293 cells in a time- and dose-dependent manne1' To examine the effec t of TAT- Hsp40 upon oxidalive stress, HEK293 cells were exposed to H2Û2. Oxidative stress induced the ra pid increase of proteasome activity foll owed by celJ death in HEK 293 cells However ‘ HEK 293 cells t 1'ansduced by TAT- Hsp40 showed r esistance against oxidative st 1'ess induced cytot oxicity, TAT- Hsp40 transduced cell s showed dec1'eased p1'oteasome activity and inhibi ted Hs p70 degradation. These results suggest that Hs p40 rnight protect cell death f rom oxida tive st1'ess by p1'ese1'ving the cellula1' level of Hs p70.
25.
2007.04
구독 인증기관·개인회원 무료
Sang Im Lee, Jun Lee, Hwa, Jeong Lee, Jong Dae Park, Sun Kyung Lee, Young Gab Yun, Byung Hun Jeon, In Soo Ree, Eun Cheol Kim
Sulfur is commonly used in Asia as an herbal medicine to treat inflammation and cancer , a nd potent chemo preventi ve effects have been demons tra ted in various in vivo and in vitromodels for s ul fur-containing compounds found in natura l1y occurring product s. Here, we 1'eport the growth inhibitory and apoptosis-related effects of a n ewly developedhigh-puri ty edible sulfur(ES) on immo1'tali zed human o1'al ke1'atinocytes(IHOKs) and on oral cancer cells representing two stages of oral cancer (HN4‘ HN12) based on an 3-(4,5-Dimethylthiazol-2-yl) -2.5- diphe n yltetrazolium bromide(MTI) assay, Western blotting, cell cycle analysis, and nuclear staining. The puri ty of the ES used in thi s study was ve1'ified by high performance liquid chromatog1'aphy (HPLC) , ami no acid analysis and energy dispersive spectroscopy(EDS). ES inhibited the prolife1'ation of imrnortalized and ma lig nant o1'al kerati nocytes in a dose- and time-dependent manne1' FITC-Annex.in V staining, DNA fragmentation t esting. and Hoechst 33258 s taining revealed that ES inhibits cell growth via apoptosis. ES bl ocked cell-cycle prog1'ession at t he sub-Gl phase‘ wi th decreased expression of cyclins Dl, D2‘ and E, and their activating partn ers cdk2‘ cdk4‘ and cdkfì, and a concomitant induction of p53 and p21/WAF1. Furthe1'more, ES treatment in creased the cytosolic level of cytochrome c and resulted in caspase- 3 activation‘ and thi s effect was co1'1'elated with Bax up-regulation and Bcl-2 down-1'egulation Taken together‘ these data suggest that ES is a potential chemopreventive and chemotherapeut ic agent fo r oral ca ncer
26.
2007.04
구독 인증기관·개인회원 무료
Human saliva contains a large number of proteins and peptides whose composition may alter as a consequence 。f disease. To date. however‘ the proteins and peptides that routinely populate thi s ora l fluid a re largely unknown To provide a catalogue of saliva proteins, we have surveyed the unstimulated human whole saliva by us ing shotgun proteornics. For the shotgun approach, whole saliva proteins were digested into peptides with ChemDigestD ‘ and the resul ting peptide fragments were separated by RP-HPLC, followed by each fraction was t ryptic digestion. ChemDigestD-Trypsin digested peptides were analyzed by tandem mass spectrometry(MS/MS) us ing a nano-LC eq 버 pped quadrupole-time of f1ight mass spectrometer, and the obtained spectra were searched against human protein seq uence data base using MASCOT. Shotgun proteomics allowed a total of 291 human pr。 teins to be confidently assigned . The largest group(17 .2%) of the identified proteins sorted into functional catego ries was included in t he signal t ransduction funct ion except for the hypothetical 0 1' unknown function. This work provides a valuable s ta rting point for the analysis of human salivary proteins and their biological functions and candidates from human whole sali va that may prove to be of diagnostic and therapeutic significance
27.
2007.04
구독 인증기관·개인회원 무료
Epithelial-mesenchymal transition(EMT) plays a pivotal role in the convers ion of earl y s tage tumors into invasive malignancies‘ and has been shown to be regulated hy the transcri ptional factor. Snail. Recent ly‘ actlvatlon of the phosphatidylinositol 3' kinase (PI3K)/따<:T axis is emerging as a centra l feature of EMT‘ However. it is unclear whether the phosphorylation of AKT regulate the expl'ession of s nail in ora l cancer cell underwent EMT. T。 investigate a role of p-AKT in EMT, we assessed the effects of inhibi ting p-AK1' activity in oral squamous can cer cells(KOSCC-25B) using PIAs, structurally modified phosphatidyli nositol ether lipid analogues(P1As) . PIAs de creased phosphorylation of c-Jun N-terrninal Kinase(JNK) and increased phosphorylation of glycogen synthase kinase 3beta(GSK-3beta). Inhibition of p-AKT ir빼ce d down regulation of Snail and Twist. but Sip1 regulated independent of p-AKT inhibition. Also inhibi tion of p-AKT dec reased cell migration and invas ion. Therefore our results implicate that p-AKT may contribute to the translocalization of sna il in the EMT associated with canceJ cell rnigration and invasion
28.
2007.04
구독 인증기관·개인회원 무료
Amelobl astic carcinoma(AC) is a maJignant coun terpart of ameloblastoma. histologically characteri zed by amelobJastomatolls feature with obviollS cytologic a typi a . AC is a r are malignant tumor with poten t iaJ of metastasis‘ asssllmed to occur far less tha n 1% of ameloblastoma . Nearly 80% of ACs have been reported to occur in the mandibJ e. The allthors reviewed 5 cases of AC in Department of Oral PathoJogy. SeouJ National University from 2005 to 2007 clini copathologically according to age, sex. location and clinical findings. They occllrred at the age from 46 to 75 years wi th average age of 60 Four cases occurred in male and 1 in female‘ showing prediJ ection for ma le‘ While four cases occu rred in the maxilla , including 3 cases in the maxillary molar area and 1 in the maxiJJ a ry premola l‘ a rea, only 1 case occurred in the mandibJe. AC showing predorninance in the maxilla over t he ma ndible in our cases in contrast to other case reviews. Three patients presented painful large oral lllcer at the time of admi ssion, a nd other 2 patients presented swol1en mass around the gingivae and alveoJar bone While the clini cal impression of 3 patients were ma lignant tllmor, other 2 patients were amelboJastoma and nicotine stomatitis. The dllration of symptoms before final diagnosis has ranged from 4 months to 2 years Radiogr a phicalJy they showed poorly demarcated radiolucent lesion with irreg비 ar bony destruction. Resected tumors were measured as 6.0X4.5x4.0cm‘ 0‘ 7xO.5xO.3cm, 5.0 x 4.5 X5.0cm. 0.8xO.6 XO.5cm, and 5cm diametel mass respecti vely. Microscopically lymph node metastasis was confirmed in 1 case, but not in others . The tumors showed basic histologic featu re of ameloblastoma with apparent cytologic atypia sllch as pleomol‘ phism‘ hyper chromatism and atypical ffiJ tOSIS Individua l neoplastic cells displayed wide va riations from case to case‘ showing c lear cells with faint PAS positive granules‘ isolated round cells with abundant cytoplasm. s quamoid ceJls and polygonaJ cell s . [mmunohis toc hemical survey was done in 2 cases. all of which showed positivity to pan-cytoker atin a ntibody and p53 a ntibody‘ but negativity to HMB-45, S-100, and SMA One case recurred twice during 2 yeal's after surgery. But 3 cases showed no evidence of recurrence and metastas is. lt seems to us t hat AC have the potential of metastasis and reCllrrence.
29.
2007.04
구독 인증기관·개인회원 무료
T:raumat ic eosinophili c granuloma(TEG) of the oral mucosa is considered to be a reactive benign lesion. which commonly manifests as an ulcer with elevated and indurated borders Clinically, this lesion simulates a malignant tumor. Histology shows a diffuse. dense, polyrnorphic, and eosinophil - rich cellular infiltra te. which extends deeply into the underlying soft t issues. A major constituent of infil trates is a population of mitotically active‘ la rge‘ atypi cal mononuclear cells. Immunohistologic evaluation of the large atypical cells has suggested a myofibroblastic 01' his tiocytic ol'igin. However, recent reports have shown that these cells are positive 1'01' CD30 antigen and it has been s llggested tha t a subset of TEG cOllld be included within the spectrum of CD30+ lymphoprolifer ative disorders. We have described 2 patients who had oral mllcosal lesions with features of TEG. ln patient 1. the lesiona l cells expressed CD3 .. CD43, LCA. Interestingly, the large cells were strongly CD30 positive. bl1t nega tive for CD68, CD45Ro‘ CD56, CD20. This case was interpreted as a CD30+ Iymphoproliferative disorder. In pa tient 2‘ t he la rge cell s showed strong posit ive for CD68, hut negative for CD30 The small lymphocytic cells ex prerssed CD3 This case was interpreted as an atypical histiocytic granuloma. Therefore, TEGs inclllde atypical histiocytic granllloma a long with the CD30+ lymphoid lesions. These findings suggested that TEG w0111d be a hete rogenous category of oral mucosal di sorders
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