본 연구는 한우를 공란우로 이용시 효율을 최대화하고, 과도한 호르몬의 투여를 억제하며, 그로 인하여 공란우의 경제수명을 연장하고자 과배란 처리시 차기 발정을 고려하지 않고 과배란을 유도하였을때 공란우의 반응과 수정란의 생산율을 조사하였으며, 그 결과는 다음과 같다. 1. 한우 경산우 및 미경산우를 자연발정을 확인하고 차기발정 5일전에 FSH를 1일 2회 4일 간 투여한 것과 발정을 확인하지 않고 CIDR를 이용하여 동일한 호르몬을 방법으로 과배란을 처리

본 연구는 한우 난구 복합체의 체외성숙에서 OA가 미치는 영향에 대하여 조사하였다. 도축 한우암소의 난소로부터 난구 복합체를 채취하여 PVA-TCM199로 3회 이상 세정 후 PVA-TCM 199, 0.2 uM, 2 uM, 20 uM OA를 각각 첨가하여 공기, 에서 6, 12, 24시간 동안 체외성숙을 실시하였다. 또한 체외성숙시 cycloheximide(CX)와 OA와 체외성숙 효과를 확인하기 위하여 0.1M-PVA TCM199, CX 25 ug/m

본 연구는 ICSI후 돼지 난자의 활성화 처리와 ICSI전 주입정자의 수정 능력 획득 유기효과를 구명하기 위하여 실시하였다. ICSI후 ethanol, cycloheximide 그리고 ethanol과 cycloheximide를 병용처리 시 난할율과 배반포배 발달율이 대조구와 처리 구간에 유의적인 차이가 없었다(p<0.05). 그러나 ICSI전 caffeine과 Ca-ionpphre로 주입정자의 수정능력 획득 유기 처리 시 난할율과 배반포배발달율 모두 처

KASI (Korea Astronomy and Space Science Institute) is developing the near-infrared camera system named KASINICS (KASI Near-Infrared Camera System) which will be installed at the 60cm f/13.5 Ritchey-Chretien telescope of the Sobaeksan Optical Astronomy Observatory (SOAO). The camera system is optimized for JHKL bands and has a 6 arcmin FOV. The optical system consists of two spherical mirrors and a 8-position filter wheel. With the exception for the dewar window, all optical elements are cooled inside cryogenic dewar. Since the Offner system is adopted to prevent thermal noises from outside of the telescope primary mirror, the secondary mirror of the Offner system acts as a cold Lyot stop. The optical performance does not change by temperature variations because the Aluminum mirrors contract and expand homogeneously with its mount. We finished the design and fabrication of the optical parts and are now aligning the optical system. We plan to have a test observation on 2006 January.

As biennale exhibitions has been expanded into all of the world since 1990s, these trends of blockbuster exhibitions have caused several problems. For instance, some major curators monopolized most global size exhibitions despite of a variety of cultural and historical backgrounds. Besides, due to a strong connection between these curators and their own artists, the young emerging artists’ opportunities tend to be reduced as a result of the power game. In addition, major curators’ power have influence on the exhibition style as well as on the theme itself. Some artists who did not involved that kind of huge scale exhibitions dispute that the direction of the exhibition is concentrating on the curator’s interest instead of artists or viewers. Although these dissatisfactions could not portray correctly the process of organizing and managing system of a biennale exhibition, those biennale exhibitions held in recent have shown tautologic discourses without any passion and positive attitude direct to the exploitation of our society as a vanguard. In the process of comparing several kinds of biennale exhibitions, I could find that some artists who participated several biennale exhibitions at the same time did not present their creative vision, although the triumph of an exhibition was typically measured by the amount of visitors. Thus, the aim of this article is to prove that the biennale can show us new cultural discourse as well as progressive method of understanding our times. Is biennale producing the real ‘global standard’? If biennale has done it, could this global standard present up-to-date paradigm for the unique exhibition system? Is biennale providing an useful opportunity for the understanding and communicating of contemporary art through the re-contextualization which is pronounced by the publicity of curator and organizing committee? How can we find the distinctive strategy from each biennale exhibition including Venice Biennale? Biennale, as a blockbuster exhibition, always requires a degree of hype, otherwise it would not be a special event and would not attract a big enough audience. It is the actual reason why major biennale exhibitions seem to be similar artistic events. Unfortunately, it seems that the excess of biennale exhibitions might bring about the lack of contents. In this case, the biennale syndrome would being a kind of the center of poverty, in spite of the visual splendor. After all, following the global standard may not be a matter of great importance now. What really matters is how each biennale exhibition which started under the different conditions can search their own identity.

콩에 큰 피해를 주는 가로줄노린재의 형태적 특징 및 온도별 발육특성을 조사하였다. 알은 2줄씩 콩잎이나 꼬투리에 산란하고 원형에 가까우며, 암컷 성충의 체장은 9.8 mm, 수컷 성충은 8.7mm로 암컷이 수컷에 비하여 크다. 부화율은 20, 25, 30 및 에서 로 온도가 증가함에 따라 높아졌으며, 알의 평균발육기간은 20, 25, 30 및 에서 각각 10.7, 5.0, 4.0 및 3.0일이다. 에서 1령, 2령, 3령, 4령 및 5령 약충의 평균발육기간은 각각 3.2, 3.4, 3.4, 3.3 및 5.9일로 온도가 증가 할수록 발육기간이 짧아졌다. 각 태별 발육영점은도와 유효적산온도는 알은 와 65.5일도, 약충은 와 322.8일도, 알에서 약충까지는 와 386.4일도였다. 에서 산란전기간은 10.0일, 성충수명은 암컷이 52.5일, 수컷이 38.2일로 암컷이 수컷에 비하여 수명이 길었으며, 이때 성충 한 마리의 총 산란수는 496개였고, 난괴수는 21.5개였다.

복숭아혹진딧물의 섭식행동을 관찰하기 위해, 고추, 무, 배추, 가지, 참외 등 5종류의 기주식물에서 EPG기술을 이용하였다. 복숭아혹진딧물의 구침이 식물체 표면에 접촉된 후 전기적 연결이 일어나기까지 소요되는 시간과 맨 처음 potential drop이 일어나기까지 소요된 시간, 구침의 세포간극내에서의 활동에 의하여 일어나는 potential drop의 수, 전기적 연결신호가 나타난 시점부터 체관부를 섭식하는 순간까지 소요된 시간 등을 조사한 결과 Potential drop 수에서는 기주들 사이에 유의성 있는 차이를 나타내었다. 그러나 나머지 요인에서는 기주식물들 사이에 큰 차이를 보이지 않았지만, 참외나 배추보다는 고추, 무, 가지에서 즘 더 기주선호성과 관련된 섭식패턴들을 확인할 수 있었다.

본 연구는 제7차 교육과정 고등학교 한문교과서를 대상으로 현토문의 문제점을 지적하고 그 개선안을 제시하고자 하였다.고등학교 한문교과서에는 현토문을 수록하였는데 문장의 의미를 정확하게 반영하지 못하고, 읽기에도 적합하지 않으며, 한문 본래의 틀을 유지하지도 못하는 문제점을 안고 있다. 심지어 현토문인지 국한문 혼용문인지 구분하기 어려운 부분도 있다. 이에 본고는 현토의 문제점을 體言用言尊稱의 오류 등으로 구분하여 고찰하였다.아울러 이러한 문제점의 해결 방안으로 휴지(休止)와 토(吐)의 조화를 추구할 것, 전통적으로 사용해온 토를 발전적으로 계승할 것 등 두 가지를 제안하였다.

We have invented mobile HEPA filter unit of hemopoietic stem cell transplantation clean room (class 100), which is a capsule type to protect the patient from being infected by harmful Aspergillus spore. This equipment is based on the basic structure of US Federal Standard 209D, biological clean room standard of NASA NHB 5340-2 and measurement standard of Japanese JACA No. 24-1989. Performance of HEPA filter was evaluated by dioctyl phthalate (DOP) test method, resulting in 99.97% efficiency for 0.3 ㎛ particles. HEPA filter unit used only 98 W electricity with 24 V storage cell for the patient's treatment for over three hours. When the wind speed was 0.2 to 0.3 m/sec as in bone marrow transplantation clean room, the air flow was constant laminar vertical type. When the amount of dust was measured in the hospital, the equipment maintained the grade of clean class 100 and microbe was not detected. The noise level was less than 52 dB(A). The amounts of microbe inside and outside the building were almost same. The amount was even more in cancer sick room, serious case room and X-rays room than in the general rooms. Also, elevators and stair room were found to transport dust to other areas. Even though the patient with low immunity can be protected from Aspergillus spore by a HEPA filter unit, the system preventing microbe system such as BMT clean room should be placed in rooms such as PET, CT, MRI, and radiation room so that the patients can be protected from having infected by the extremely harmful Aspergillus spore.

The number 01' patien ts with tongue carcinoma is increasing rapidly among young indiv idua ls in many parts of the worl d. Until now‘ most of studies were focused on the comparison malignancy wi th normal 01' dysplasia. There is little report of gene a lterations in normal to cancer , oral carcinogenesis. The purpose of present study is to evaluate the gene a ltc ration in every steps of oral carcinogenes is by DD- PCR. To induce tongue carcinoma in rat by 4- NQO. each dri nking water made 10ppm. 25ppm. 50ppm and control(o nly D.W without 4-NQO) . Specimens were classified into 4 groups s uch as co ntrol, I(mild & mocle rate dysplasia) , II(severe dysplasia and car cinoma in s itu) , III(carcinoma). Total RNA was ext racted and DD- PCR was performed using customized random primers. And to confïrmed t he results of DD-PCR‘ RT- PCR a nd real-time PCR with specific primers were carried out. There was phenotypic alteration in tongue 。f dosc a nd t imc dc pcndcnt man ncr. In gross examination, multiple papules, patch form or ulcerations were observed during 4 - NQO t reatment Hi s tologicall y, dysplasia was observed in 3 to 6 month and tumor formation in 6 to 8 month For DD-PCR, RT-PCR and real-time PCR, cyclophilin A, BAC RP23-372MB and BAC CH230-103E9 were differ entia lly expressed. Taken together, cyclophilin A has a role in all steps of oral carcinogenesis. BAC RP23-372N田is implica ted in carcinoma in s itu a nd BAC CH230-103E9 mRNA expression is assoicated with dysplasia and carcinom in s itu Conclus ively. some genes a re impli catcd a ll st eps of oral carci nogenesis, others are associated with one step, whi ch meant that genes are di fferentia lly expressed in every steps