Many transcription factors are involved in directing the growth of porcine oocytes. The localization and expression level of a given transcription factor often differ at each stage of early embryonic growth, which spans from fertilization to the formation of the blastocyst. A hallmark of the blastocyst stage is the separation of the endodermal and mesodermal ectoderm. The embryo's medium and its effects are known to be crucial during early development compared to the other developmental stages, and thus require a lot of caution. Therefore, in many experiments, early development is divided into the quality of oocyte and cumulus cells and used in experiments. We thought that we were also heavily influenced by genetic reasons. Here, we examined the expression patterns of five key transcription factors (CDX2, OCT4, SOX2, NANOG, and E-CADHERIN) during porcine oocyte development whose expression patterns are controversial in the pig to the literature. Antibodies against these transcription factors were used to determine the expression and localization of them during the early development of pig embryos. These results indicate that the expressions of key transcription factors are generally similar in mouse and pig early developing embryos, but NANOG and SOX2 expression appears to show species-specific differences between pig and mouse developing embryos. This work helps us better understand how the expression patterns of transcription factors translate into developmental effects and processes, and how the expression and localization of different transcription factors can crucially impact oocyte growth and downstream developmental processes.

The aim of this study was to compare the two in vitro culture systems callus and adventitious root by investigating the biomass and phenolic compounds in calli and adventitious roots induced from four different explants (leaf, root, petal, and ovary) in Camellia japonica. The biomass of calli and adventitious roots was examined after 4 and 8 weeks of cultivation, respectively, and 22 phenolic compounds were analyzed using high-performance liquid chromatography (HPLC). The biomass of the ovary-derived calli (2.0 g･mass-1) was 1.5-fold that of the leaf-derived calli. The dry weight (DW) was highest in ovary-derived calli; however, the highest dry matter content was obtained from leaf-derived calli. Differences in the investigated characteristics depended on the callus origin. In adventitious roots, the highest biomass was achieved in the leaf-derived adventitious root system; its fresh weight was 2.3-fold (89 mg･ea-1) higher, and its DW was 1.8-fold (16 mg･ea-1) higher than those of ovary-derived adventitious root system. Active cell division was detected in petal-derived lines in both the calli and adventitious roots. Results of the HPLC analysis revealed that the total content of 22 phenolic compounds was highest in ovary-derived calli and ovary-derived adventitious roots. Our experiments confirmed that the calli and adventitious roots of C japonica have different cytological characteristics and bioactive compounds depending on the explant origin. In addition, callus culture was a more suitable system than adventitious root for producing phenolic compounds when the duration of the culture period and biomass were considered.

The purpose of this paper is to compare seafarers’ behavior according to traffic conditions of a road and an onshore locations. Behaviors are classified into three categories: Skill-, Rule- and knowledge-based mode. Experimental data were collected using the questionnaires for navigators, working in a merchant ship. To compare the behaviors, we used the four analysis method; the degree of frequency, reliability test, correlation and linear regression. As a result of the study, it was found that Skill-based behavior shows more higher in the road traffic than the maritime traffic, and rule-based behavior shows more higher in the maritime traffic than the road traffic. Also, the behavior in the navigation situation showed statistical significance. Especially, in the case of Rule-based behavior, a high correlation between road and maritime was found. This study can be expected to apply to complementary system utilization between error management system of onshore and maritime traffic.

Family Tineidae, belongs to the superfamily Tineoidea, with about 2,500 described species belong to 450 genera, and 16 subfamilies all over the world (Robinson, 2009). Classification of this family studied by Zagulyaev (1972), Robinson (1988), Davis & Robinson (1998), Nieukerken et al. (2011), and Regier et al. (2015), etc. The family was reported 33 species belonging to 19 genera of eight subfamilies in Korea up to date (Paek et al., 2010; Park et al., 2012; Byun et al., 2014; Lee et al., 2016; 2017a; 2017b; 2018). In this study, a total of 42 species belonging to 22 genera of nine subfamilies are recognized in Korea. Among them seven species are reported from Korea for the first time, and two species are described new to science.