The H2S 22,0 - 21,1 line emission is observed to be strongly localized toward Sgr B2(M), and emissions from other positions in the more extended SgrB2 region are almost negligible. H2S is thought to form effectively by the passage of the C-type shocks but to be quickly transformed to SO2 or other sulfur species (Pineau des Forets et al. 1993). Such a shock may have enhanced the H2S abundance in Sgr B2(M), where massive star formation is taking place. But the negligible emission of H2S from other observed positions may indicate that these positions have not been affected by shocks enough to produce H2S, or if they have experienced shocks, H2S may have transformed already to other sulfur-containing species. The SO2 222,20 - 221,21 line was also observed to be detectable only toward the (M) position. The line intensity ratios of these two molecules appear to be very similar at Sgr B2(M) and IRAS 16239-2422, where the latter is a region of low-mass star formation. This may suggest that the shock environment in these two star-forming regions is similar and that the shock chemistry also proceeds in a similar fashion in these two different regions, if we accept shock formation of these two species.

This study was carried out to investigate the general characteristics and viability of sperm after freezing and thawing and the pregnancy rates after artificial insemination with thawed semen. The rates of viable sperm after slow and rapid freezing were 87.4±3.85% and 70.8±4.45%, respectively which were significantly lower than that of fresh semen control (91.7±3.45%). The mean concentration of epididymal sperm after dilution in 1.0 ml saline and. 3.0 ml extender in a various concentrations of cryoprotectants was 124.5±48.3 x 10/sup 6/ (range of 45 x 10/sup 6/ to 280 X 10/sup 6/ /ml). There was a significant difference not in the percentage of acrosome-reacted sperm, but in the percentage of capacitated sperm, between fresh and frozen-thawed epididymal semen. When frozen-thawed after diluting with tris-buffer extender containing glycerol, DMSO and ethylene glycol with concentration of 2 to 6%, the rates of epididymal sperm exposed to different cryoprotectants ranged from 14.4±4.7% to 20.7±5.8%, 17.8±5.2% to 36.5±4.9%, and 14.4±4.6% to 18.5±5.3%, respectively which were lower compare to fresh semen control. The pregnancy rate after artificial insemination with frozen semen was 70.6%, whereas that with fresh semen was 90.0% in dogs with naturally induced estrus.