Cordyceps militaris mycelium extracts containing high amounts of cordycepin were evaluated in vitro for their antiinflammatory and tumor cell growth-inhibitory activities. All extracts dose dependently inhibited the increased production of inflammatory mediators including reactive oxygen species (ROS), nitric oxide (NO), and β-hexosaminidase in lipopolysaccharide (LPS)-stimulated inflammatory cells. All extracts were evaluated for anti-proliferative activity against normal RBL-2H3 cells and diverse types of cancer cell lines, including HCT, MC5-7, U-87MG, AGS, and A549 cells. The extract showed the strongest growth inhibition (IC50 = 28.13 μg/mL) relative to vehicle-treated control cells against fibrosarcoma (MC5-7). We have demonstrated anti-inflammatory activity of C. militaris via inhibition of NO, ROS production, and β-hexosaminidase release in activated cells. C. militaris mycelium extract was also evaluated mechanistically and found to exert six types of anti-cancer activity, confirming its pharmacological potential. Our study suggests C. militaris use as a potential source of anti-inflammatory and anticancer agents. C. militaris may also be considered a functional food.

Store-operated Ca2+ entry (SOCE) represents one of the major Ca2+ entry routes in non-excitable cells. It is involved in a variety of fundamental biological processes and the maintenance of Ca2+ homeostasis. The Ca2+ releaseactivated Ca2+ (CRAC) channel consists of stromal interaction molecule and Orai; however, the role and action of Homer proteins as an adaptor protein to SOCE-mediated Ca2+ signaling through the activation of CRAC channels in non-excitable cells still remain unknown. In the present study, we investigated the role of Homer2 in the process of Ca2+ signaling induced by the interaction between CRACs and Homer2 proteins in non-excitable cells. The response to Ca2+ entry by thapsigargin-mediated Ca2+ store depletion remarkably decreased in pancreatic acinar cells of Homer2–/– mice, as compared to wild-type cells. It also showed critical differences in regulated patterns by the specific blockers of SOCE in pancreatic acinar cells of Homer2–/– mice. The response to Ca2+ entry by the depletion in Ca2+ store markedly increased in the cellular overexpression of Orai1 and STIM1 as compared to the overexpression of Homer2 in cells; however, this response was remarkably inhibited by the overexpression of Orai1, STIM1, and Homer2. These results suggest that Homer2 has a critical role in the regulatory action of SOCE activity and the interactions between CRAC channels.

The sorption of Eu on MX-80 bentonite in Na–Ca–Cl solutions is investigated at a molal proton concentration (pHm) range of 3 to 10 and an ionic strength (I) range of 0.1 to 6 m (mol·kgw−1). The sorption equilibrium of Eu on MX-80 is achieved within 14 to 21 d at I = 0.1 and 6 m. The sorption distribution coefficient (Kd) values of Eu for MX-80 increase as pHm increases from 3 to 6 for all I values, and they are independent of pHm between 8 and 10 at I ≥ 0.5 m. Meanwhile, at I = 0.1 m, the Kd value at pHm = 10 is slightly lower than those at pHm = 8 and 9. The Kd values are not affected by the I values between 0.5 m and 6 m, whereas the Kd value at I = 0.1 m is greater than those at I ≥ 0.5 m, except at pHm = 10. A two-site protolysis nonelectrostatic surface complexation and cation exchange sorption model is applied to the Eu sorption data for I ≤ 4 m, and the equilibrium constants of the sorption reactions are estimated.

Coal-based graphite has become the main material of emerging industries. The microstructure of coal-based graphite plays an important role in its applications in many fields. In this paper, the effect of carbon disulfide/N-methyl-2-pyrrolidone solvent mixture extraction on the microstructure of bituminous coal-based graphite was systematically studied through preliminary extraction coupled with high-temperature graphitization. The graphitization degree g (75.65%) of the coal residue-based graphite was significantly higher than that of the raw coal-based graphite. The crystallite size La of the coal residue-based graphite was reduced by 47.06% compared with the raw coal-based graphite. The ID/ IG value of the coal residue-based graphite is smaller than that of the raw coal-based graphite. The specific surface area (16.72 m2/ g) and total pore volume (0.0567 m3/ g) of the coal residue-based graphite are increased in varying degrees compared with the raw coal-based graphite. This study found a carbon source that can be used to prepare coal-based graphite with high graphitization degree. The results are expected to provide a theoretical basis for further clean and efficient utilization of the coal residue resources.

With the rapid improvement of hardware and software-related IT technology, applying A.I. to the private and public sectors, such as the Food Poisoning Prevention Program in Nevada and Smart City based on big data in Boston, is steadily increasing. However, the cases of application to the regulation sector of government are still insufficient. The Korea Institute of Nonproliferation and Control (KINAC) is studying to apply A.I. technology to the regulation to improve the objectivity, consistency, and efficiency of classification and export licensing review. The KINAC developed the Nation Nuclear Technology Information Collection and Analysis System using A.I. techniques such as machine learning and deep learning techniques. KINAC and FNC Technology are developing the Export Risk Assessment System using A.I. modules and Bayesian Networks. The KINAC and Korea Atomic Energy Research Institute (KAERI) are developing an inventory history management system subject to the Nuclear Cooperation Agreements. The Nuclear Safety and Security Commission (NSSC) and KINAC operate the Nuclear Import and Export Control System (NEPS) for application and export license review according to relevant laws such as the Foreign Trade Act. Therefore, preparing an integration plan for the existing NEPS and the new systems is necessary. Since the NEPS has to be operated and accessible at all times, so the stability of the NEPS is the most important when integration and linking. So, it is suggested that the Collection and Analysis System and the Risk Assessment System, which require a lot of data traffic, are configured in a server separate from the NEPS, and the new DB and the NEPS DB are only linked. An inventory history management system is also suggested to be integrated and configured into the NEPS. Third, it is recommended that each system lists the information provided to or received from the NEPS in advance, and one-way communication should be performed basically. Two-way communication should be performed when necessary. Finally, against various cyber accidents and information leakage, it is proposed to review security vulnerabilities and apply essential security measures and guidelines. Through the integration and linkage of these systems, it is expected that the objectivity, consistency, and efficiency of classification and export licensing review of the KINAC are strengthened, and national transparency of development, production, and use of nuclear material is enhanced. It can be satisfied with the increasingly strengthened demands of the international community on duty for strategic item management.

The ROK conducts several export procedures, communications in connection with transfers; exchange of information on export plan, shipments, and receipt of nuclear materials, in accordance with bilateral Nuclear Cooperation Agreements (NCA) and Administrative Arrangements (AA) signed with US, Canada, and Australia. Also, the inventory amount of items subject to NCA has reported annually. This study reviewed the export procedures and management methods for spent nuclear fuel subject to NCA. The re-transfer procedures start with obtaining consent from the original exporting country. It is impossible to retransfer nuclear material without consent, whether long-term or individual case-bycase. If the material has multiple obligations, prior consent from all of those countries is required. Therefore, it is necessary to clarify the foreign obligated materials correctly. In general, nuclear fuel is subject to multiple obligations of all countries through which the materials have passed during the front-end fuel cycle. Then the new obligations are imposed on those irradiated materials or their by-products after ‘used-in’ or ‘produced through the use of ’ equipment subject to NCA. For example, fuel assemblies manufactured under CANDU fuel fabrication equipment subject to ROK-Canada NCA or burned in nuclear reactors where US equipment is installed have obligations based on Canada or US agreements. In order to impose obligation to irradiated materials, the principle of proportionality is applied as stipulated in each Agreement. According to the AA between US and ROK, nuclear materials used in the equipment transferred under the Agreement and produced through them are differently controlled. After the cycle in the reactor with US-made equipment, uranium in the irradiated fuel is considered a material used in the equipment. So it would be appropriate to apply obligation proportionality according to its origin, regardless the US-made equipment. Meanwhile, the obligation under US NCA is given to the entire amount of produced plutonium in the irradiated fuel. Although the contribution to the production of fuel is to be discussed case-by-case basis in the case of Canadian obligation, applying a similar method is proper. Since the fuel is burned in the form of bundles or assemblies, it is impossible to separate the spent fuel into uranium and plutonium physically. However, as discussed above, to clarify the rights and obligations pursuant to Agreement and ensure accuracy in inventory management, the obligation codes should be imposed on irradiated fuel as not a single item but separated individual substance of materials. Moreover, when an obligation swap occurs for the irradiated fuel, its movement and combustion history should be considered to prevent confusion in confirming multiple obligations and implementing export procedure.

The Nuclear Safety Act defines items defined in nuclear cooperation agreements with each country as internationally regulated materials and requires that import and export procedures be implemented according to each agreement. In particular, the US, Canada, and Australia, which are major nuclear power suppliers, describe detailed procedures related to imports and exports in administrative agreements attached to the agreement. This paper compares and analyzes the import and export procedures of agreed items in the three major countries and proposes procedures and precautions that nuclear companies should follow for smooth import and export. The import/export procedure, according to the annexed administrative agreement, is divided mainly into Direct-transfer, Indirect-transfer, and Re-transfer. Direct transfer refers to direct import and export between countries that have signed an agreement. The four-step process of prior notice, written confirmation, shipment notice, and receipt confirmation is commonly required for direct transfer. However, in the case of Canada, if the previously related information is the same, the shipping notification and receipt confirmation procedure may be omitted. Australia only defines items included in the notification without a separate form in the administrative agreement. Indirect transfer means transferring items originating from the other two countries that signed the agreement through a third country. All three countries stipulate that item transferred by indirect transfer are also subject to the agreement, and Canada stipulates separate advance notifications and shipping notifications for indirect transfer. Australia manages indirect transfers by including information from third countries in the exchange-related information between the two countries. The US does not have a specific procedure for indirect transfer, but it is presumed that it was omitted because it was difficult to confirm the time of shipment when the US exports through a third country. Re-transfer is a procedure to obtain consent from the original exporting country in advance when exporting items held in Korea to a third country. There are two types of consent based on re-transfer, the long-term consent method, and the individual case-by-case method. Long-term consent is a method of long-term consent for re-transfer to an agreed-upon country by agreeing in advance on a list of countries where re-transfer is possible. In the long term, the procedure will be reflected in domestic laws in detail and managed through an IT-based management system so that operators can smoothly implement such complex import and export procedures.

Meat affects color and quality by metabolite concentrations. Meat produces metabolites, and metabolites are caused by a variety of causes. Meat also produces metabolites by oxidation, which is an inevitable chemical process that meat undergoes which is resulting information of various chemical compounds. Thus, the aim of this study was to profiling the change of metabolites of M. longissimus lumborum during the storage at 4°C. Instrumental color measurements were showed decreasing chroma value, redness and yellowness (P<0.05) during storage, while non-significance (P>0.05) changes found in lightness value. Above all, hue angle was highest at 21 d of storage (P<0.05). The lipid and protein oxidation of muscles was measured by TBARS value significantly increased (P<0.05), thiol and carbonyl groups were also increased significantly (P<0.05) during the display. Total 19 of 60 identified compounds appeared to have a significant difference by storage time (P<0.05). Hue angle had a significant correlation with specific metabolites such as carbon disulfide, 3-methyl-1-butanol, 2-ethyl-1-hexanol, lactic acid and palmitic acid (P<0.05). Results of the current study provide the conversion of volatile and non-volatile metabolites and their correlation with oxidative indicators for changes in meat quality during aerobic storage.

Plant-derived compounds have been reported to possess anti-inflammatory abilities contained inhibited β-hexosaminidase, ROS and NO release. Essential oils are natural volatile complex compounds that are characterized by a strong scent and produced by aromatic plants as various plant-derived compounds. The essential oil extracted from Zanthoxylum coreanum Nakai (Z. coreanum) has various functional properties; however, little information is available regarding its anti-allergic inflammatory. A total of 17 compounds were detected in Z. coreanum oil, and the main component was estragole (50.86%). The tested Z. coreanum oil and estragole statistically inhibited the release of β-hexosaminidase induced by antigen stimulation in RBL-2H3 cells. This Z. coreanum oil and estragole may stimulate the secretion of active oxygen (ROS) or nitric oxide (NO) which are considered to involved in anti-inflammatory events. Moreover, it is suggested that Z. coreanum oil and estragole may negatively control the production of SNARE proteins (VAMP7) at the tran-scriptional and translational levels in common. These results demonstrate that Z. coreanum oil and its major component, estragole, possess potent anti-inflammatory abilities that are coupled with antioxidant properties.

Cordyceps militaris, a well-known traditional Chinese medicine, has multiple health-promoting effects. It is used as a herbal remedy and health food in Asian countries. Cultured mycelia are often used as a substitute for natural C. militaris. In the present study, the mycelia and fruiting bodies of artificially cultured C. militaris were analyzed using a metabolomics approach. The protein and crude fat contents of the mycelia were substantially higher than those of the fruiting bodies. The top three abundant amino acids in the mycelia were proline (3.9 g/100 g), aspartic acid (2.9 g/100 g), and glutamic acid (2.7 g/100 g). The carbohydrate content was similar in the fruiting bodies and mycelia. Analysis revealed that both the fruiting bodies and mycelia are rich in phenolic compounds and exhibit antioxidant activity. Further, six metabolites were significantly different between the mycelia and fruiting bodies. The levels of Ca, glucose, Mg, and Se were higher in the mycelia than in the fruiting bodies. In contrast, mannitol and Zn were more abundant in the fruiting bodies. The current study provides a comprehensive metabolic profile of the mycelia and fruiting bodies of artificially cultured C. militaris. Such an exercise is potentially important for understanding the metabolism of C. militaris and facilitating the use of cultured mycelia as a supplement to C. militaris fruiting bodies in traditional Chinese medicine.

Conductive carbon cloths (CCs) have been great attention as a promising current collector for flexible supercapacitors that supply power to portable and wearable electronics. However, the hydrophobic surface and weak adhesion with active materials has limited to be adopted as the binder-free and flexible electrode with mechanical/electrochemical stability. In this work, we demonstrate preparation of binder-free and flexible electrodes based on polyaniline (PANI) on carbon cloth. Polydopamine (PDA) layer are used to impart hydrophilicity, leading to uniform growth of PANI on the hydrophobic surface of carbon. Furthermore, PDA layer improves adhesion strength between PANI and carbon substrates, which allows for superior mechanical stability under ultrasonic condition. PANI-based flexible electrode shows high areal capacitance (160.8 mF cm− 2 at 0.5 mA cm− 2), good rate capability (71.1% even at high current density of 10 mA cm− 2), and long-term cycling stability (82.6% capacitance retention after 1500 cycles). Furthermore, a quasi-solid-state flexible supercapacitor reveals remarkable mechanical flexibility and durability, with superior capacitance retention (~ 100%) in bent state and after repetitive 1000 cycles.

Gabion was unfavorable to the reconstruction of the landscape caused by limit plant growth because of commonly filled with stone. Therefore, gabion was need substrate as possible to plant growth for harmonize with the surroundings and prevent of erosion. The objective of this study was to evaluate the effect of substrates based growing media containing wood meal on physical property (moisture content), chemical properties (organic matter and C/N ratio), plant cover and plant species in gabion. The substrates were composed of soil and soil mixed with growing media (9:1 (v/v) and 4:1 (v/v)). The growing media was prepared by mixing the basic materials, such as peat, perlite, with the specific materials, such as steam treated wood meal (Quercus mongolica) and NH4NO3. Field experiments were carried out on a damaged forest slope, South Korea, from January to November. The results, the moisture content of soil mixed with growing media (4:1, v/v) was maintained high value than others during the thermophilic period and consistently high organic matter which at an advantage for long term plant growth for 11 months. We were confirmed soil mixed with growing media (4:1, v/v) was an advantage to keep C/N ratio for plant growth period. The growing media based substrates has high plant cover percentage and advantage to plant growth for long term. And to conclude, in general the project is a success considering that few repairs or follow up will be necessary. This technique offers a solution to create a better harmony with the surrounding environment.