기름치를 참치회 또는 메로구이로 판매하는 사례가 있으며 국내에서는 2012년 6월1일부터 식품원료로 판매가 금 지되어 이를 판별하는 시험법 마련이 필요하다. 기름치는 농어목(Perciformes) 갈치꼬치과(Gempylidae)에 속하는 기 름갈치꼬치(R. pretiosus)와 흑갈치꼬치(L. flavobrunneum)가 있으며 이를 판별하기 위한 종 특이 프라이머를 개발하기 위하여 미토콘드리아에 존재하는 16S DNA 유전자부위를 선정하였다. 그리고 미국 국립보건원에서 운영하는 유전자 은행(www.ncbi.nlm.nih.gov)에 등록되어있는 기름갈치꼬치, 흑갈치꼬치. 참다랑어, 황다랑, 청새치 및 황새치의 염기서열을 대상으로 BioEdit ver. 7.0.9.0 프로그램을 사용하여 비교 및 분석을 실시하였다. 분석을 통하여 기름갈치 꼬치 및 흑갈치꼬치를 판별할 수 있는 각각 4종의 프라이 머를 설계하였다. 설계된 프라이머에 대하여 대조군으로 다랑어 3종(참다랑어, 황다랑어, 눈다랑어) 및 새치류 4종 (청새치, 황새치, 녹새치, 돛새치)에 대한 실험적 평가를 실 시하였다. 그 결과 기름갈치꼬치에 대하여는 R.P-16S-006- F/R.P-16S-008-R, 흑갈치꼬치는 L.F-16S-004-F/L.F-16S- 006-R 프라이머를 최종 선정하였으며, PCR 조건을 확립하였다. 확립된 조건에서는 각각 178bp 및 238bp의 PCR 산 물을 확인하였으며, 유사종간의 비특이적 밴드는 형성되 지 않았다. 따라서 본 연구에서 개발된 기름치를 판별할 수 있는 종 특이 프라이머는 인터넷쇼핑몰 또는 시중에 불법적으로 유통 가능성이 있는 제품을 신속하고 과학적으로 판별할 수 있어 식품안전관리에 활용도가 매우 클 것 으로 기대된다.

In this study, ribulose bisphosphate carboxylase (rbcL), RNApolymeraseC (rpoC1), intergenic spacer (psbA-trnH), and second internal transcribed spacer (ITS2) as identification markers for discrimination of P. mirifica in foods were selected. To be primer design, we obtained 719 bp, 520 bp, 348 bp, and 507 bp amplicon using universal primers from selected regions of P. mirifica. The regions of rbcL, rpoC1, and psbA-trnH were not proper for design primers because of high homology about P. mirifica, P. lobata, and B. superba. But, we had designed 4 pairs of oligonucleotide primers from ITS2 gene. Predicted amplicon from P. mirifica were obtained 137 bp and 216 bp using finally designed primers SFI12-miri-6F/SFI12-miri-7R and SFI12-miri-6F/SFI12-miri-8R, respectively. The species-specific primers distinguished P. mirifica from related species were able to apply food materials and processed foods. The developed PCR method would be applicable to food safety management for illegally distributed products in markets and internet shopping malls.

홍삼에서 사포닌 추출과정 중 부산물로 얻어지는 홍삼오일의 화학적 특성 및 화장품소재로서의 응용가능성을 알아보고자 하였다. Tween 80으로 희석한 1% 홍삼오일의 산가는 0.265, 과산화물가는 0.387, 요오드가는 0.64이었다. DPPH free radical scavenging assay 결과, 1% 홍삼오일은 대조군인 동일 농도의 α-tocopherol에 비해 70% 정도의 항산화능을 나타내었다. 홍삼오일의 total polyphenol 함량은 243 mg/100 g이었다. Tyrosinase저해 활성을 측정한 결과, 홍삼오일은 대조군인 L-ascorbic acid에 비해 14% 정도의 활성억제 효과를 나타내었다. 홍삼오일에 의한 elastase 활성억제 효과는 거의 없었으며 홍삼오일의 Staphylococcus aureus와 Escherichia coli 에 대한 생육억제 효과도 없었다.

In this study, the experimental method has been investigated using molecular biological way to identify raw materials from seasoned red-pepper sauce which is one of the most popular spices in Korea. 6 kinds of seasoned red-pepper sauces were chosen as a sample containing chilli pepper, garlic, onion as a major ingredient and species specific primers were used for the identification of the raw material of processed food. Selected samples were pre-treated to remove salt (samples were washed with distilled water 3~4 times for desalting), after that, to amplify the extracted genes, whole genome amplification (WGA) kit was performed. Afterwards, PCR products were confirmed through the electrophoresis. As a result, 102, 180, 280 bp of specific PCR products were confirmed for each major ingredients such as chilli pepper, garlic, onion. From this study, the gene extraction method was validated for the identification of ingredients from the spices and it would be applied to distinction of low quality chilli pepper powder including seasoned red-pepper sauce illegally.

In this study, effective gene extraction methods were compared to identify raw materials of processed meat products through molecular biological methods. Species specific primers were used to identify ingredients of processed foods and, as a sample, 13 kinds of processed meat products including beef, pork and chicken. According to the type of sample, 13 kinds of samples were classified into liquid type, source type and powder type. The samples were pre-treated (centrifugation) and (or) performed Whole Gene Amplification (WGA) kit for amplification of the extracted DNA. As a result, it was possible to identify the raw material of products through the centrifugation of sample 1 ml for liquid type of processed meat products. For source type of products after gene extraction, it was required to perform WGA for the identification of ingredients. For powder type products did not required any further pre-treatment and WGA. In this study, it was an opportunity to confirm the possibility of identification of raw material from the gene extraction of processed meat products and this method could be used to examine the authenticity of raw material of products.

The purpose of this study was to examine the appearance of norovirus in the water for food in food service institutions and the influence of physicochemical and microbial factors of norovirus in order to work out basic data to predict the detection of norovirus. Among 82 samples of water for food in food service institutions, norovirus appeared in 7 samples and the rate of appearance was 8.5%. As for the type of norovirus, one samples contained GI type (genotype GI-6) and six samples contained GII type (genotype GII-2, GII-4, GII-12). In the regression model of prediction of norovirus, the rate of appearance was correlated with NH₃-N, total solids and the consumption of KMnO₄, out of such variables as NH₃-N, total solids, the consumption of KMnO4, depth, chloride and total colony counts, and its contribution rate for effectiveness was 78.60%. In order to examine the influential factor of environment upon the detection of norovirus, Pearson's correlation analysis was carried out. The predictable regression formula for appearance rate of norovirus was expressed as -1.818 + 42.677 [NH₃-N] + 0.023 [total solids] + 0.762[consumption of KMnO₄] -0.009 [depth] -0.146 [chloride] + 0.007 [total colony counts] (R = 0.904, R² = 0.818,adjusted R² = 0.786, p < 0.05). The most influential factors upon the detection of norovirus were NH₃-N, total solids and the consumption of KMnO₄. In other words, when the measured values of NH₃-N, total solids and the consumption of KMnO₄ were higher, the possibility of appearance of norovirus increased.

Patulin, a mycotoxin mainly produced by Penicillium and Aspergillus, is found in various foods. In the present, a maximum acceptable level for patulin is established at 50 μg/kg(ppb) in apple juices and apple concentrates in Korea. But patulin may be detected in foods produced with other fruits. In the present study, patulin contamination was analyzed in 520 samples of fruit juices and beverages, and 50 samples of fruit juice concentrates. High performance liquid chromatography(HPLC) was applied to quantitatively analyze patulin levels in samples and liquid chromatography-mass spectrometry(LC/MS/MS) was used to remove false positive results. The results showed that three samples of 520 fruit juices and beverages and five samples of 50 fruit juice concentrates were contaminated by patulin, 9.8 - 18.0 μg/kg and 4.7 - 18.2 μg/kg respectively. Contaminated samples were produced with apple, orange or pear. This indicates that it is necessary to extend the regulatory range of patulin. In the other hands, the present study confirmed the effectiveness of LC/MS/MS analytical method to remove false positive results.

This study examined the consumers' awareness and information need toward the irradiated foods and environmental hormones. The data were collected from 350 adults living in Daegu and Busan by the self-administered questionnaire. Frequencies and chi-square tests were conducted by SPSS. The results of the survey were as follows: (1) consumers' awareness regarding the irradiated foods and environmental hormones were low, while consumers' concerns for them were high, (2) the orders of the information needs for the irradiated foods are safety of irradiated foods, dose permitted for food irradiation, benefits of irradiated foods, kinds of permitted irradiated foods, and legislations of food irradiation, and (3) the orders of the information needs for the environmental hormones are harmfulness of environmental hormones, standards for contamination by environmental hormones, materials releasing environmental hormones, methods to prevent environmental hormones, and kinds of environmental hormones.

This study was conducted to investigate the consumers' recognition and information need for food safety, especially focused on pesticide residues, foodborne illness, and food additives. The data were collected from 350 adults living in Taegu and Pusan by the self-administered questionnaire. Frequencies and chi-square tests were conducted by SPSS. The results of the survey were as follows: (1) the consumers' concerns about food safety were high, (2) many respondents worried about eating vegetables, fruits and grains in turn because of pesticide residues, and did not trust the results from food-safety tests, (3) the major factor for foodborne illness was regarded as unfreshed or contaminated food stuffs, (4) many consumers concerned highly about preservatives among food additives, and tried to consume food containing less food additives, and (5) many respondents wanted to get the information about harmfulness of pesticide residues in foods, method to choose fresh food, and safety of food additives.

The purpose of this study was to describe the levels of market goods substitution of housework and find out the determinants on it in the domain of food among married female home-based workers. The data were collected from 169 married female home-based workers in Pusan and Kyungnam province, by self-administered questionnaires. Frequencies, percentiles, Cronbach's alpha, Pearson's correlations, and multiple regression were used to analyze the data. In the convenience foods, frozen foods had the highest substitution level, whereas prepared stew had the lowest. The substitution level of Korean traditional storage foods was the middle. And in dining-out service, the substitution level was mostly high: the level of delivery service was higher than that of dining-out. Compared to the previous research, these results showed that market goods substitution tended to increase, and its level in the domain of food will promote continually over time. The variables affecting the substitution level of convenience food were the number of family members, occupation, the existence of elderly/disabled person in the family, sex-role attitude, and weekly hours at home-based work. The substitution level of Korean traditional storage foods was influenced by sex-role attitude, occupation, education, monthly household income, and the existence of elderly/disabled person in the family. The significant variables affecting the substitution level of dining-out service were weekly hours at home-based work, the number of family members, occupation, monthly household income, education, and sex-role attitude.

식품의 방사선조사 기법은 지난 40여년간 세계적으로 체계적인 연구가 수행되면서 그에 대한 안전성과 기술적 타당성이 입증되고 있음에도 불구하고 아직까지도 제한적으로 이용되고 있는데 이의 주된 이유는 방사선조사 식품에 대한 소비자의 수용도를 확신할 수 없기 때문이다. 따라서 본 연구에서는 방사선조사 식품에 대한 올바른 정보를 소비자들에게 제공하기 위한 기초자료를 얻기 위해 방사선조사 식품에 대한 소비자들의 구입의도 및 구입의도에 따른 집단을 구분하는데 영향을 미치는 변수들을 분석하였다. 본 연구를 통해 얻어진 결과를 요약하면 다음과 같다. 첫째, 우리 나라 소비자들의 식품의 안전성 및 식품의 신선도 보존에 관한 관심도는 매우 높았다. 방부제, 잔류농약, 식중독, 식품의 변패 및 식품의 신선도 보존 등의 5가지 항목 중 식품의 변패에 대한 관심도가 특히 높게 나타났으며, 그 다음으로는 잔류농약, 식중독의 순으로 관심이 많았다. 둘째, 우리 나라 소비자들의 방사선조사 식품에 대한 인지도는 대체로 낮았으며 특히 연령이 많거나 학력이 낮은 경우 방사선조사 식품에 대해 인지도가 낮은 것으로 나타나 이들을 대상으로 한 소비자교육이 보다 활성화되어야 할 필요가 있는 것으로 나타났다. 방사선조사 식품에 대한 수용 정도에 있어서는 응답자의 1/3-4/5가 방사선조사 식품의 구입에 대한 찬성이나 반대의 의사표시를 보이는 대신에 관망적인 자세를 취하고 있었다. 방사선조사 기법이 제공하는 혜택 중에서 농약 처리 후 식품에 농약이 잔류되는 것과는 다르게 방사선조사 식품에 방사선이 잔류하지 않는 경우, 방사선조사 식품을 구입할 의도가 많은 것으로 나타났다. 즉 식품의 안전성과 관련하여 많은 소비자들이 식품에서 농약의 잔류성분에 대해 우려를 하고 있는 것으로 나타났으므로 앞으로 이를 대체할 수 있는 하나의 방법으로 식품에 방사선조사 처리 기법이 활용될 것으로 예상되며, 이를 위해서는 방사선조사 식품의 안전성이 뒷받침되어야 할 것으로 보인다. 셋째, 방사선조사 식품의 구입의도 정도에 따른 집단을 구분하는데 영향을 미치는 변수를 살펴본 판별분석의 결과, 식품의 안전성에 대한 관심도, 혁신성에 대한 자아평가, 유기농작물에 대한 지불의도, 그리고 방사선조사 식품의 인지도는 방사선조사 식품의 구입의도를 구분하는 집단을 설명하는 데 있어 대체로 영향력이 있는 것으로 나타났다. 반면에 월가계소득은 5개의 판별분석에서 한 번도 유의하지 않은 것으로 나타나서 방사선조사 식품의 수용정도를 구분하는 데 있어 영향력이 없는 것으로 나타났다. 특히 소비자의 혁신성 정도가 방사선조사 식품의 수용도를 구분하는데 있어 중요한 변수라는 사실은 방사선조사 식품의 기법이나 그러한 식품이 갖는 혜택과 관련된 정보를 혁신적 소비자나 조기수용자에게 우선적으로 제공하는 것이 효과적임을 알려주고 있다. 최근 국제적으로 방사선조사 식품의 이용이 확대되고 있으며, 수출입 식품에 대한 방사선 처리가 증가되고 있는 국제환경 속에서 소비자들에게 방사선조사 식품에 대한 올바른 정보를 효과적으로 제공함으로써 그들의 식품구입 의사결정에 도움을 주는 것이 매우 필요하다. 더욱이 본 연구결과 우리 나라 소비자들은 방사선조사 식품에 대한 낮은 인지도를 가지고 있어 소비자로서의 알권리를 제대로 누리지 못하고 있는 것으로 나타나 앞으로 국내에서 방사선조사 식품이 상업적으로 널리 보급되기에 앞서 방사선조사 식품에 대한 소비자교육이 활발하게 이루어질 필요가 있다고 본다.

Food irradiation is an emerging technology which offers many advantages such as reduction of microorganisms, extension of shelf-life of foods, reduction in the use of post-harvest chemicals, and destruction of insects and parasites. The commercial utilization of food irradiation, however, has been restricted because of the uncertainty of consumers' responses to it. Because success of food irradiation in the marketplace will depend upon their acceptability by consumers, this study focused on the consumers' perception and acceptance toward food irradiation in order to get basic data for commercial utilization of food irradiation and give information to consumers to help rational consumption behavior. The survey with 411 respondents living in Youngnam area was conducted during the spring of 1997 by the questionnaires. The results and implications from this study are as follows. First, consumers' knowledge about food irradiation is scanty. Two-thirds of respondents in the survey had not heard of irradiated foods and many people confused irradiation with radioactivity. In the willingness to accept food irradiation, one-third of respondents showed a wait-and-see attitude. This result indicated consumers had insufficient information about the irradiation process and nationwide education of food irradiation technology should be undertaken. Second, although the purchase and use of food are very important consumption behaviors, consumer education by mass communication has been rarely done. For the successful commercialization of food irradiation, the information provision by mass communication for the consumers should be made. Third, consumers generally worried about residual pesticide and intended to purchase irradiated foods if radioactivity was not retained in the foods. Therefore, food irradiation could be an alternative method to the use of pesticide Fourth, consumers pointed out that they wanted to extend shelf-life of milk and dairy foods, fish and seafood and to irradiate these foods. Therefore, research for the safety of irradiated foods should be continually conducted. finally, labeling for irradiated foods is needed to provide the information and to further increase public understanding. Especially, the labeling should show the definite reason why irradiation is being used. In conclusion, recently, under the circumstances that the commercial utilization of food irradiation and irradiation for the import and export products have been increased in many countries, many efforts are needed to improve the quality of irradiated foods, and prove the safety of them in Korea. In addition, consumer education for food irradiation should be given to help consumers to make decision for food purchase and use.

The consumers' purchase experiences of some imported agricultural products (rice, sesame, garlic, onion, jujube, red pepper, oak mushroom, apple, dried persimmon) and their consciousness for the labelling of the country of origin for agricultural products in Youngnam region were examined in this study. The sample consisted of 438 women who lived in Taegu, Pusan and other cities of Kyungpook and Kyungnam, and the self-administered questionnaire for the survey was used. The main reasons to purchase the imported agricultural products were the wide distribution and low price of them. Most of the consumers were concerned about safety of the imported agricultural products but only a few people experienced unsatisfaction of the products. The price, quality, package, container, label, and safety of the examined products were evaluated worse than those of the domestic products. Generally, although they suffered unsatisfaction, the consumers did not complain because they wanted to avoid troublesome work, which means the consumers did not assert their rights. Some people did not know the labelling system for the country of origin and the place to charge the market violating it. Therefore, it will be necessary for the consumers to receive education about the labelling system for the country of origin and the compensation criteria for consumers' grievances and damages. The current labelling method for the country of origin generally met the consumers' preference but the consumers wanted to improve the position and color of the label. Most consumers could not differentiate the imported products from the domestic products. The results obtained from the survey suggest consumer education for the labelling system for the country of origin and the compensation criteria should be activated through various ways such as mass media and consumer organizations.

본 연구는 원예치료프로그램이 시설에 거주하는 치매노인의 일상생활수행능력과 손기능에 미치는 효과를 알아보고자 하였다. 2008년 3월 13일부터 5월 24일까지 3개월간 실험군을 대상으로 원예치료프로그램을 회당 2시간씩 주 2회, 총 22회 실시하였다. 원예치료프로그램은 치매노인의 일상생활수행능력과 손기능 향상을 위해 친근하고 쉬우며 다양한 손동작을 필요로 하는 작업으로 구성하였다. 일상생활수행능력 경우 대조군은 원예치료 실시 전 14.7점에서 실시 후 13.7점으로 감소하였으나 유의성은 없었다(p=0.294).실험군은 실시 전 14.1점에서 실시 후 15.7점으로 1.6점 높아졌으며 p=0.05 수준에서 유의하게 향상되었다. 악력은 대조군의 경우 원예치료실시 전 5.75kg에서 실시 후 5.43kg으로 감소하였으나 유의차는 없었다(p=0.657).실험군의 경우 실시 전 4.83kg에서 실시 후 8.38kg으로 고도로 유의하게 (p<0.01)증가하였다. 손가락 근력은 대조군의 경우 원예치료실시 전 4.01kg에서 실시 후 4.11kg으로 0.1kg 증가하였으니 유의한 차이는 없었다(p=0.811).실험군은 원예치료 실시 전 4.83kg에서 실시 후 8.38kg으로 3.55kg 증가하였으며 고도로 유의한 차이(p<0.01)를 나타내었다. 이상의 결과로부터 치매노인에게 친숙하고 쉬우며 손기능에 적절한 원예치료의 적용은 시설에 거주하는 치매노인의 일상생활수행능력의 향상뿐만 아니라 손기능의 유지와 강화에 효과적인 것으로 나타났다.