Electrical acupuncture has been shown to induce hypoglycemic effect in streptozotocin diabetic rats. The Zusanli acupoints have been widely used in traditional Chinese medicine to relieve symptoms of diabetes mellitus. But, the effects of electrical acupuncture stimulation of different frequencies are still unclear. We designed an experiment to investigate the effect of electrical acupuncture of high (100 Hz) and low (2 Hz) frequencies on Zusanli acupoint for 15 minutes in streptozotocin diabetic rats. They were divided into 4 groups, high frequency electrical acupuncture (HFEA group, n=5), low frequency electrical acupuncture (LFEA group, n=5), without any treatment (control group, n=5) and normal group (n=5). After 2 weeks of treatment, the HFEA and LFEA groups had a significantly decreased glucose concentration in serum in comparison to the control group. Additionally, the HFEA group had a significantly decreased glucose concentration in serum compared with the LFEA group. Both the HFEA and LFEA groups had a significantly decreased Immunoglobulin G concentration in serum compared with control group. The LFEA and control groups had a significantly increased cholesterol concentration in serum compared with the normal group, but the HFEA did not have a significantly increased cholesterol concentration. Thus we suggest that electrical acupuncture stimulation at the Zusanli acupoints is effective on hypoglycemic effect and immune function. Overall, the therapeutic effects of electrical acupuncture at 100 Hz was better than effects at 2 Hz.

본 연구는 단위발생유래 생쥐 배아줄기세포(P-mES)지가 체외수정유래 생쥐 배아줄기세포 (mES)와 마찬가지로 기능성 심근세포로 체외 분화되는지를 조사하였다. 각 세포주 P-mES04와 MES03를 4일간 부유 배양하여 배아체 (EB)를 형성한 다음 4일간 DMSO를 추가적으로 처리한 뒤 젤라틴이 코팅된 배양접시에 부착시켰다(4-/4+). P-mES04와 mES03으로부터 수축성 심근세포 생성 여부를 30일간 관찰한 결과, 각각 13일(69.83%)과 22일 (61.3%)에 누적 형성율이 가장 높았다. 면역 세포화학염색 결과, 수축성을 나타내는 P-mES04 세포는 수축성 mES03 세포에서와 같이 근육 특이적인 anti-sarcomeric a-actinin 항체와 심근 특이적인 anti-cardiac troponin I 항체에 염색되는 것을 확인하였다. 또한 RT-PCR 결과, 수축성을 나타내는 P-mES04 세포는 심근특이적인 L-type calcium channel, a1C, cardiac myosin heavy chain a, cardiac muscle heavy polypeptide 7β, GATA binding protein 4와 atrial natriuretic factor는 발현하나, 골격근 특이적인 L-type calcium channel, a1S는 발현하지 않아 웅성 성체의 심장세포와 유사한 양상을 보였다. 본 연구의 결과는 단위발생 유래 생쥐 배아 줄기세포를 배아줄기세포의 연구의 대체제로 이용할 수 있음을 보여준다.

This study examined the role of interpretation with various practices in art museums to seek a new meaning and a concept of art museum today. The exploration of interpretation would be a starting point to discuss about on art museums with professionals in each art-related field. While museums recognize the concept of interpretation and the scope of the functions in different levels, the study focused on the practices of collecting and exhibiting that will entrust the museum new realms of activities toward the audience. In particular, its emphases are set force on the information on the collections via the museum’s web sites, interpretation policies, and theories and methodologies in exhibition development. Art museum websites well reflect how museums utilize the new medium to enhance the understanding of art works by providing in-depth art historical information, comprehensive contexts, and subject/concept based search methods. In recent decades, these have enacted changes to expand dimensions of interpretive functions in most museums, particularly in the United States and others. In an administrative perspective, Tate Gallery Interpretation Policy became an good example how an art museum put its interpretation philosophy as the basis of interpreting collection and public programs. Tate established functions of intrepretation and education not only within a task-based team but also as an intrer-divisional coorperation to provide an interpretation scheme of information provisions such as guide brochure, audio tour, multimedia content, and library. New environment and trends of museum exhibition, and its development processes stem from communication theories, object interpretation philosophy, display strategies, and various evaluation techniques through audiences. With the communication theories of Shannon and Weaver, Berlo’s SMCR(Source-Message-Channel-Receiver) models were perceived as to understand the mechanism to communicate museum exhibits to visitors. Suzan vogel’s insight into object display strategy helped to conceive the mechanism of object recontextualization. She emphasized that the museum’s practice to construe opinions and impressions through object display should be discreet and critical. therefore, the professionals to plan the exhibition should reveal the intention and their practices. For a prevailing new methodology from the field, the interpretive exhibition development processes are articulated as the front-end, formative, and summative evaluation, futhermore the team process in industrial product management models was adapted. These have turned out to be more interactive with visitors and effective to communicate the exhibition concepts and messages, hence resulting in enriched museum experiences. Finally the study concluded that understanding the aspects of interpretation should help art museums to set a framework for current practices to expand its public dimension. It can provide curators with a critical view to website planning and its content. And obviously, the interpretive exhibition development methodology will lead museum exhibition developers to be skilled in its current approaches to thematic exhibition concerning diverse subjects and topics.