본 연구는 bisphenol A의 실제 식품을 통한 사람의 노출농도에서 에스트로젠 효과를 알아보기 위하여 사람의 유방암 세포인 MCF-7와 난소를 적출한 무흥선 마우스를 이용하여 살펴보았다. MCF-7세포를 이용한 genistein과 bisphenol A가 용매대조군과 무처치대조군에 비하여 genistein(10nM, 100nM, 1 uM, l0 uM)과 bisphenol A(2 ng/㎖, 4 ng/㎖, 8 ng/㎖, 16ng/㎖)의 세포성장촉진 효과가 용량의 증가에 따라 증가하는 경향을 보였다. genistein의 경우 1 uM을 정점으로 세포성장촉진이 극에 달하다가 l0 uM 투여군에서 1 uM 투여군에 비하여 약 40% 정도 세포성장이 감소하였으나 대조군에 비하여 여전히 세포의 성장을 촉진하였다. 이러한 결과는 bisphenol A와 genistein이 에스트로젠과 같이 에스트로젠 수용체와 결합하는 경로로 MCF-7세포의 성장을 촉진한 것으로 생각된다. 따라서 이러한 결과를 확인하기 위하여, 에스트로젠 의존적으로 유전자가 발현되는 유전자 중의 하나인 pS2 유전자의 발현을 살펴보았다. Genistein과 bisphenol A가 pS2유전자의 발현을 유도하는 것이 northern blot분석에 의하여 관찰되었다. 또한, 21일령의 무흉선 마우스를 이용한 in vivo연구에서, 무처치 대조군에 비하여 genistein과 bisphenol A를 투여한 마우스 군에서 유선의 발달과 end-bud의 형성이 유의하게 증가됨이 관찰되었다(p<0.05). 결론적으로, bisphenol A와 genistein이 MCF-7 세포에서 에스트로젠 효과가 매우 낮은 농도에서도 나타날 수 있다는 것은 식품위생상 큰 의미를 갖고 있으며, 이러한 물질들의 실제 사람에서의 위해성 특히 성장기 유아의 위해성에 대하여 좀 더 면밀히 연구되어야 할 것이다.

Various antimicrobial drug screen tests have been used in order to ensure food safety. However, the conventional screen teats, the Swab Test on Premises(STOP, USA), the Calf Antibiotic and Sulfa Teat(CAST, USA) and the European Economic Community 4-plate Test(FPT, EU) are not sufficiently rapid or sensitive enough to detect low levels of sulfa drugs in meat. We developed a new screen test kit for the determination of the antimicrobial residues in meat called the Bacillus megaterium Disk AssayBmDA). A comparison of BmDA with the older screen tests showed BmDA was as good as the older ones with several advantages. The new test kit is faster-it can be read in 4-6 hours instead of 16-18 hours. Moreover, BmDA can discriminate sulfa drugs from other antimicrobial drugs because p-aminobenzoic acid countacts the inhibiting action of sulfa drugs. Minimum detectable levels of sulfa drugs were significantly improved at the level of 0.025-0.1 ppm compared with the level of 1.0 ppm in FPT. A comparison of BmDA with the older screen tests in HPLC confirmed meat samples exeeded the Korean tolerance value of 0.1 ppm showed BmDA was the most sensitive in the microbiological screen tests. As the microbiological screen tests have already known, a person familiar with simple laboratory techniques should have no difficulty in using it to detect antimicrobial residues in meat. This would be a simple, economic method of antimicrobial residues detection which might be succesfully used by many laboratories.

This study was surveyed by 319 male and 336 female college students in Seoul for food behavior and effect of Western food on food life. The results were summarized as follows; 1. The recognition of Korean folk food was higher in female than male students. 2. Eating type of college students appeared that they prefered to cooked rice at breakfast, lunch, and dinner because of a general food type of their family. 3. The favorite food items in college students showed fruits in female and meat in male. 4. Eating out type with family appeared mainly Korean food, because of their preference. 5. The 29% of college students visited fast food restaurants more than 1~2 times per week. 6. In compared to Western food, the Korean food were favored by college students but cooking process of food were complicated. 7. The preference of Korean food against Western food was mostly higher in male than female. 8. The standard of food choice were taste and convenience of food. 9. Main factor of food habit change appeared convenience of buying easily. 10. The influence of the Western food in our food behavior were easy to buying but not fit our preference. 11. Opinion of students in order to keep Korean food were follows; Various food development, enlargement of consumption market, correct education about Korean food and change of people consciousness through consumer campaign.

The objective of this study was to access the effects of sensory and physical properties of Beandaeduk on 7 different combination methods (three were combined with mung bean and different proportions of water; four were combined with mung bean and various cereals with constant water), and 3 storage periods (0, 1 and 3 weeks at -18℃). This experiment was consisted of sensory evaluation I (appearance, texture, taste, flavor and overall preference), II (color, moisture, volume, hardness, elasticity, viscosity and ratio materials, off-flavor and oder) and physical analysis of each sample, and questionaire survey of Beandaeduk. In sensory evaluation I except flavor, significant differences (p〈0.05) were found among different combination methods of sample. Also significant differences (p〈0.05) were found in sample for storage periods, except appearance. In sensory evaluation II, only moisture and oder of samples were significantly different (p〈0.05) for storage periods, and there were significant differences (p〈0.05) in samples among different combination methods, except elasicity. Stepwise regression for storage periods in sensory I showed that texture and taste were important factors in overall preference of sample. In the questionaire survey, preference and adding materials of Beandaeduk were different according to province. Half of respondents preferred to get instant Beandaeduk and instant mung bean powder.