Bemisia tabaci is a vector of Tomato yellow leaf curl virus (TYLCV) but Trialeurodes vaporariorum is not. To determine the effect of TYLCV acquisition on vector and non-vector, we compared various physiological characteristics between two species. Our results showed that TYLCV acquisition significantly affected B. tabaci but not T. vaporariorum. The B. tabaci increased susceptibility against thermal stress but weakened chill coma recovery, shortening of longevity, low fecundity and abbreviated developmental time by virus acquisition. At the molecular level, B. tabaci increased hsp70 and hsp90 levels by TYLCV ingestion. However, T. vaporariorum did not shown any changes of those characteristics. Therefore, the physiological manipulation of TYLCV was specific to vector species but not non-vector species.