The larvicidal activities of 11 Myrtaceae essential oils and their components against Aedes aegypti were tested by the immersion method. We also tested the acute toxicity of 4 active oils and their components against the water flea, Daphnia magna. Further, the aqueous residues of these oils and their components were determined at 2 and 7 days after suspending in water. Among the 11 oils tested, 0.1 mg/mL of Melaleucalinariifolia, M. dissitiflora, M. quinquenervia, and Eucalyptus globulus oils showed strong larvicidal activity against A. aegypti. Among the test compounds, allyl isothiocyanate, γ-terpinene, p-cymene, (+)-limonene, (-)-limonene, γ-terpinene, and (E)-nerolidol showed strong larvicidal activity against A. aegypti. The acute toxicity test revealed M. linariifolia was the most toxic to D. magna. Among test compounds, allyl isothiocyanate was the most toxic to D. magna. Two days after treatment, the residues of M. dissitiflora, M. linariifolia, M. quinquenervia, and E. globulus oils in water were 55.4, 46.6, 32.4, and 14.8%, respectively. Low concentrations of allyl isothiocyanate, γ -terpinene, p-cymene, (-)-limonene, (+)-limonene, and γ-terpinene were detected in the water at 2 days after treatment. Therefore, Myrtaceae essential oils and their components could be developed as control agents against mosquito larvae.

This study was carried out to compare the major volatile components in essential oil from different origin of Atractylodes spp. which is being traded as a crude herbal drug in Korean herbal markets. From the two Atractylodes of major volatile components were similarly detected such as the β-selinene, β-sesquiphellandrene, germacrene B, 2,7-dimethoxy-2-methylnaphthalene and 9-methoxy-2,3-dihydrofuro3,2-qcoumarin. Among the volatile components, the major components were 2,7-dimethoxy-2-methylnaphthalene (40.98%), 9-methoxy-2,3-dihydrofuro 3,2-q coumarin (15.74%), and β-sesquiphellandrene (1.98%) in both Atractylodes. As a results, It was found that the two Atractylodes were the same species which was being traded in the Korean herbal markets as the A. japonica. not to different species of A. japonica and A. macrocephalla, respectively.