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        검색결과 1

        1.
        2018.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Dianthus caryophyllus (carnation) is a globally important ornamental plant. Tissue culture techniques have been used for the commercial production of carnation; however, the micropropagation of carnation has been impeded due to the occurrence of hyperhydricity during the shoot multiplication process or micropropagation stage 2. In the present study, the effects of different concentrations of rare earth elements on the reduction of hyperhydricity in micropropagated carnation were investigated. Nodal explants of D. caryophyllus ‘13827’ were cultured on Murashige and Skoog medium containing 1.0 mg·L-1 6-benzyladenine and 0.5 mg·L-1 indole-3-acetic acid with 3.0% (w/v) sucrose and 0.8% (w/v) agar (control medium). The medium was supplemented with lanthanum nitrate, La(NO3)3, cerium nitrate, Ce(NO3)3, or neodymium chloride, NdCl3 at a concentration of 0.05, 0.1, or 0.15 mM. Hyperhydricity was observed in 68.9% of the cultures produced on the control medium. The lowest percentage (42.2%) of hyperhydricity was observed in plants propagated on the medium supplemented with 0.05 mM Ce(NO3)3. Furthermore, the soluble protein concentration was higher in both the non-hyperhydric and hyperhydric plants produced on the medium supplemented with 0.05 mM Ce(NO3)3, whereas the activity of catalase, guaiacol peroxidase, and ascorbate peroxidase was lower in comparison with the media lacking Ce3+. The results of this study suggest that supplementation of the culture medium with 0.05 mM Ce(NO3)3 alleviates oxidative stress and reduces hyperhydricity in carnation during the adventitious shoot multiplication stage.
        4,000원