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        검색결과 16

        1.
        2017.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Staphylococcus (S.) aureus is commonly found on the skin and mucous membranes of animals. Moreover, some isolates producing staphylococcal enterotoxins (SE) are also responsible for food poisoning. This study was conducted to explore the prevalence of S. aureus enterotoxin from slaughtered pigs and cattle. A total of 202 carcass swabs were collected from slaughterhouses: 102 samples were taken from slaughtered pigs and 100 were taken from cattle, respectively. Among them, 16 (7.9%) from slaughtered pigs were found to contain S. aureus, while S. aureus was not isolated from any of the slaughtered cattle samples. Additionally, six (37.5%) of the S. aureus isolates contained genes that encode staphylococcal enterotoxin type A. Therefore, it is necessary to investigate the management of food-borne pathogens based on differences in the process by which pigs and cattle are slaughtered.
        3,000원
        3.
        2011.12 구독 인증기관 무료, 개인회원 유료
        The objective of this study was to determine the effects of E. coli isolated from porcine semen on sperm viability, motility, and semen pH. Semen samples were prepared using commercial extender, SeminarkPro (Noahbio Tech, Korea) that did not contain antibiotics. And 4 different levels of E. coli were artificially innoculated to semen with following concentrations; 4,000 of sperms with 1 of E. coli (T1), 400 with 1 (T2), 40 with 1 (T3), and 4 with 1 (T4). Semen samples were preserved at 17℃ for 5 days in semen storage box until analyzed by flowcytometer. Aliquots were subjected to measure the sperm viability (Live/Dead® stain), motility (mitochondrial function), and semen acidity (pH) from day 0 (day of semen collection) to day 5. Sperm motility and viability were significantly decreased (p<0.05) on day 0 (4 hrs after preservation at 17℃) in T3 and T4 compared to control groups and were significantly decreased (p<0.05) in all groups from day 3. Sample pH was acidic in T3 (6.90~6.86) and T4 (6.86~6.65) from day 3 to day 5 (p<0.05). On the other hand, sample pH was maintained 7.0~7.1 in control, T1, and T2 during the experimental period. Sperm motility and viability were significantly decreased from day 0 to day 5 compared to control in samples contaminated with E. coli above a value of 40:1 (20×106 sperm cells/ml : 5×105 cfu/ml). Even on day 1 in T4 and on day 3 in T3, semen pH was acidic probably due to the acidification of dead spermatozoa. These results suggest that E. coli contamination has a concentration-dependent detrimental effect on extended porcine semen quality.
        4,000원
        4.
        2011.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Erysipelothrix rhusiopathiae is a causative agent of erysipelas in avian and mammalian species globally. Eight dead 5-week-old Ross broiler chickens were submitted for diagnostic tests in September 2005. Typical signs including white necrotic foci on the liver, focal congestion of the pancreas, severe atrophy and hardening of the bursa of Fabricius were detected. E. rhusiopathiae was isolated from haemorrhagic lung samples. However, no specific PCR product for the viral diseases tested was detected in these chicken samples. To our best knowledge, this is the first report of the isolation of E. rhusiopathiae in broiler chickens in Korea.
        3,000원
        5.
        2011.06 KCI 등재 구독 인증기관 무료, 개인회원 유료
        There are many researches about the contribution of virginiamycin use in animals to quinupristin/dalfopristin (Q/D) resistance in humans. In this study, the prevalence and mechanisms of streptogramin resistance in Enterococcus faecium from chickens were investigated. A total of 170 E. faecium isolates from 38 chicken farms was tested for antimicrobial susceptibility. Eleven (6.5%) E. faecium isolates were found to be resistant to Q/D. The vatE and ermB genes were detected in 2 (18%) and 6 (55%) of Q/D-resistant E. faecium respectively. By using pulsed-field gel electrophoresis (PFGE), 6 distinct PFGE patterns were identified. These data indicate that Q/D resistance among E. faecium from chickens remains low despite the long history of virginiamycin use. However, we have to concern over antimicrobial resistance in bacteria originated from animals, including the possibility of transfer of resistance genes from animal to human.
        4,000원
        6.
        2011.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The present study was conducted to investigate serotype distribution and biofilm formation of Actinobacillus (A.) pleuropneumoniae isolated from pneumonic lungs of pigs. A total of 37 A. pleuropneumoniae were isolated between January 2009 and June 2010. Serotypes of A. pleuropneumoniae isolates were determined using two different PCRs. The majority of isolates belonged to serotype 5 (n=31, 83.8%), and the others belonged to serotype 1 (n=4, 10.8%), and 2 (n=2, 5.4%), respectively. The ability of biofilm formation of the isolates was also determined by quantitative microtiter plate assay. Biofilm formation was observed in both 23 (62.2%) of the 37 field isolates and seven (43.8%) of the 16 reference strains. On the other hand, biofilm formation was various according to the serotypes: 20 (64.5%) of serotype 5, and three (75.0%) of serotype 1. However, two isolates of serotype 2 did not produce the biofilm in this study. Consequently, A. pleuropneumoniae serotype 5 was the most frequently detected (83.8% of the isolates), and 23 (62.2%) of 37 isolates exhibited biofilm positive phenotype in this study.
        4,000원