Inaccurate amounts of active ingredients in disinfectants can result in inefficiency or in potential toxicity to the environment and living organisms. This may lead to disinfection failure, and consequently, biosecurity failure. To ensure the efficiency of disinfectants and their ongoing compliance with safety and quality requirements, continuous and systematic post-market surveillance studies are needed. Herein, the content of ten commercial disinfectants purchased in 2021 was analyzed. Selective analytical techniques, such as automated titration, colorimetry, and high-performance liquid chromatography, were used to evaluate the content of several active ingredients present in disinfectants, such as potassium peroxymonosulfate, benzalkonium chloride, glutaraldehyde, phosphoric acid, citric acid, and malic acid. The obtained values were then compared with the label claims; the active ingredient contents of all disinfectants were within the acceptable range of 90–120% of the label claim.

Vaccinations, surveillance, quarantine, and disposal of the infected poultry are the common strategies for prevention and control of the highly infectious poultry diseases; however, many pathogens still persist and are potential causes threatening the nationwide spread of poultry diseases. A strict biosecurity strategy including disinfection is the key to control the spread of avian pathogens, such as the Newcastle disease virus (NDV). It is important to select and use the disinfectants whose efficacy and the effective concentrations against the specific pathogens are known. Therefore, in the present study, we evaluated the virucidal efficacy of five active substances of commercial disinfectants, namely potassium peroxymonosulfate (PPMS), sodium dichloroisocyanurate (NaDCC), glutaraldehyde (GLT), benzalkonium chloride (BZK), and didecyldimethylammonium chloride (DDAC), that are used against NDV. Further, we validated the efficacy of eight pre-approved disinfectants. The minimal virucidal concentrations of the active substances against NDV were as follows: PPMS, 2.50 g/L; NaDCC, 2.00 g/L; GLT, 0.40 g/L; BZK, 2.00 g/L, and DDAC, 1.00 g/L. Furthermore, all the eight disinfectants were found to be effective against NDV at the recommended concentrations, thereby confirming that the active substances are functional against NDV. This is the first study reporting the virucidal activity of the active substances of commercial disinfectants against NDV, in accordance with the Animal and Plant Quarantine Agency guidelines, in South Korea. The results of the virucidal efficacy testing of chemical disinfectants from this study will help poultry industries implement improved strategies for controlling infection.