This investigation aimed to assess the appetite response changes of olive flounder to starving and re-feeding conditions. Three different feeding groups (2 weeks feeding, fed; 2 weeks starving, starved; and 1 week starving and 1 week feeding, re-fed) were established to examine the changes in appetite-related genes for each group. The weight gain of the fish was highest for the fed group and lowest for the starving group. Based on the daily feed intake (DFI) and cumulative feed intake (CFI), overall food intake was found to increase in the re-fed group more than in the fed group from week 1 to week 2 of the experiment. Hypocretin neuropeptide precursor (HCRT) and galanin receptor 1 (GAL-R1) mRNA expression in the brain of olive flounder were decreased in the starved group. Corticotropin-releasing hormone (CRH) was decreased in all experimental groups, except for the fed group. However, overall leptin concentrations in the plasma did not change across groups. Considering the differences between this study and previous studies on starving and feeding, various factors (except the production and expression mechanisms of appetite-related factors in response to starving) are likely acting on the appetite responses of the fish. In this study, a 1-week re-feeding period induced substantial effects on appetite response when compared to a 2-week feeding period. These findings show that even if re-feeding is performed after starving, the unbalance caused by the re-feeding can affect various physiological changes in fish by feed intake efficiency.

This study investigated cytogenetic and hematological and histological characteristics between diploid and spontaneous triploid on Cyprinidae (Crucian carp, Carassius auratus; crucian carp, C. cuvieri and common carp, Cyprinus carpio) in four major rivers of Korea. In our results, DNA contents of triploid Cyprinidae were 50% more than those of diploid Cyprinidae. Also, erythrocyte size of triploid Cyprinidae was 50% larger than those of diploid Cyprinidae. In all sampling rivers, sex ratios of C. auratus were biased to female, and especially, triploid groups of C. auratus were all female groups (p <0.05). In principle, sex ratios of C. cuvieri and common carp were equivalent between male and female.

We determined the effects of different water temperatures (15, 20, and 25℃) and photoperiod cycles (24L:0D, 12L:12D, and 0L:24D) on the oxygen consumption of the offspring of a cultured Japanese strain (JJ), a selected Korean strain (KK), and intraspecific hybrid strains (JK and KJ) of red seabream, Pagrus major, under starvation conditions. The different fish strains, water temperatures, and photoperiod cycles had effects on the mean oxygen consumption of fish. Oxygen consumption increased with increasing water temperatures for all photoperiod treatments (p <0.001). Fish held in continuous darkness (0L:24D) used consistently less oxygen than fish exposed to continuous light (p <0.05). The oxygen consumption of fish exposed to the light phase in a 12L:12D photoperiod was higher than that of fish in the dark phase of the 12L:12D cycle, and differences were significant in three of the strains: JJ (15℃), KK (15 and 20℃), and KJ (25℃). The oxygen consumption of the inbred (JJ and KK) and intraspecific hybrid (JK and KJ) strains varied with differing water temperatures and photoperiod cycles. The JK strain displayed significantly higher oxygen consumption than the other strains under all experimental conditions except 15℃ with a 0L:24D photoperiod. The JK and KJ strains usually showed the highest and lowest oxygen consumption values, respectively, whereas the inbred strains exhibited intermediate values. Oxygen consumption in the JJ and JK strains was usually higher than that of the KK and KJ strains. We propose that differences in the thermal sensitivity and photosensitization properties of the strains contribute to differences in their ability to adapt to changes in water temperature and photoperiod, thus resulting in differences in the amplitude of their metabolic rates.

Kisspeptin (Kiss) and its cognate receptor, kisspeptin receptor (KissR; G protein coupled receptor 54, GPR54), have recently been recognized as potent regulators of reproduction in teleosts. Additionally, leptin plays an important role in energy homeostasis and reproductive function in teleosts. The purpose of this study was to examine differences in the concentration of the hormones of the Kiss/KissR system and leptin and the expression of their underlying genes, all of which are involved in the sexual maturation of female goldfish, Carassius auratus, following treatment with Kiss. The expression levels of KissR increased after the Kiss injection. Furthermore, the peptide hormone leptin also increased after the injection (in vivo and in vitro). Additionally, the expression of GnRH and GTHs (GTHα, FSHβ, and LHβ) increased in the brain and pituitary (in vitro and in vitro). These results support the hypothesis that Kiss plays important roles in the direct regulation of the hypothalamus-pituitary-gonad axis and leptin in goldfish. Therefore, we suggest that Kiss system gene expression is correlated with energy balance and reproduction.

The human ELAV(embryonic lethal abnormal vision)-like protein HuR stabilizes a certain group of cellular mRNAs that contain AU- ri ch elements in theil‘ 3’• untranslated region, Dysregulation of mRNA s tability may be relevant in tumor biology and may lead to abnormal expression of several proteins in malignant tumors, The aim of this study is to identify the differentially expressed proteins according to the functi onal activi ty of HuR Methods : We used stabl e expression of small interfering RNA(siRNA) of HuR gene to inhibit the expression of HuR in human ora l car cinoma cell lines‘ KB cell line and YD10B cell line‘ and compared the proteomic changes between s iRNA-treated and cont rol cell line using two-dimensional gel electrophoresis , Flow cytometry caliber scan(FACS) was employed to investigate the effects of HuR on cell apoptosis and proliferation , Results: Seventeen differentially expressed proteins between the two cell lines were identified by electrospray ioruzati on quadrupole time-of- fl ight mass spectrometry(ESI-Q-TOF MS) and database searching, Among them there are eleven proteins which are significant ly up- regulated in siRNA- treated cell line, which include heat shock protein 10(influencing nucl eocytoplas rnic transpo 다， cell dedifferentiation , and inhibition of apoptosis) , keratin 19(basal cell differ ent iat ion) ‘ and nucleoside diphosphate kinase B(G protein activator), etc Enolase 1 and Ml of pyruvate kinase a re the representatives of signifi cantly down-regulated proteins in siRNA-treated cell 11l1e Conclusion : Our data suggest that HuR participate in mRNAs stability of proteins that have the counter effects in the carcinogenesis of oral ca ncer , And the functiona l proteomics a re needed to elucidate the detailed interactions between HuR and t hese molec ules

p16INK4A and p15INK4B tumor suppressor genes are frequently altered in various human tumors. Hypermethylation of the promoter region of p16INK4A and p15INK4B seem to be the major mechanism of inactivation. To determine whether the change in p16INK4A and p15INK4B methylation status occur in oral squamous cell carcinomas (OSCCs) and benign oral epithelial hyperplasias, we analyzed 46 OSCCs and 20 benign oral epithelial hyperplasias by methylation-specific PCR. We also analyzed a subset of the samples for p16INK4A and p15INK4B protein expression by immunohistochemistry. The promoter region of p15INK4B was hypermethylated in 13 specimens of the 15 finally analyzed OSCCs and three specimens of the five analyzed benign oral epithelial hyperplasias. By immunohistochemical analysis, we confirmed the loss of p15INK4B expression of all hypermethylated specimens. The promoter region of p16INK4A was amplified by both an unmethylated- and a methylated-specific primers in just one OSCCs. The remaining specimens including 11 OSCCs and four benign oral epithelial hyperplasias were normally methylated. By immunohistochemistry, we analyzed the loss of p16INK4A expression in seven specimens of the 12 OSCCs and two specimens of the four benign oral epithelial hyperplasias. Except for one OSCC, however, all specimens showing loss of expression were normally methylated. These results suggest that loss of p16INK4A and p15INK4B protein expression play an important role in the development of both OSCCs and benign oral epithelial hyperplasias.

골반저근은 골반기관을 지지하는 기능을 가지고 있으며 요자제를 유지하는 여성의 주요 하부조직이다. 골반저근의 약화는 복압성 요실금의 원인이 되는데, 이러한 골반저근의 기능 정도는 복압성 요실금의 병증정도를 평가하는 지표로 사용될 수 있다. 이에 본 연구에서는 골반저근의 수축 압력을 측정하여 복압성 요실금의 병적 진행정도를 정량적으로 진단할 수 있는 요실금 진단 알고리즘을 제안하였다. 이를 위하여 골반저근의 수축압력 정보를 측정할 수 있는 시스템을 제작하였으며, 측정된 데이터의 특징 분석을 위한 측정 프로토콜을 제안하였다. 복압성 요실금 환자로부터 획득한 데이터를 이용하여 5개의 진단 파라미터를 추출하였으며, 이를 이용한 진단 알고리즘을 구현하였다. 임상시험을 통하여 진단 알고리즘의 정확성을 평가한 결과 80%의 정확성을 보였으며, 20%의 위양성 진단 결과를 보였다. 반면에 위음성 진단 결과는 확인되지 않았다. 본 연구에서 제안한 요실금 진단 알고리즘은 복압성 요실금의 병적 진행 정도를 정량적으로 진단할 수 있으며, 요실금 진단 시스템 개발에 활용될 수 있을 것으로 판단된다.

샌프란시스코 평화조약은 연합국과 일본 사이에 체결된 법적 구속력 있는 합의문서로서 제2차 대전 이후 아시아태평양에서의 새로운 국제질서인 샌프란시스코 체제의 법적 정점이다. 전후 국제질서에 대한 국제적 법 체제(legal system)로서 샌프란시스코 조약체제는 카이로선언과 포츠담 선언 그리고 이를 수락한 일본 항복문서, 연합국총사령부(SCAP)가 제정하여 공포한 법적 구속력 있는 명령들(instruments)과 샌프란시스코 평화조약, 그리고 샌프란시스코 평화조약 체결 이후의 국제적 합의와 관행을 포함하는 광범위한 국제적 법적 합의의 체제로 이해할 수 있다. 영토분쟁의 당사국들이 제시하는 다양한 근거에 대한 국제법원의 실행을 살펴보면 영토 주권 또는 국경관련 분쟁에 대하여 권원(title)을 입증할 적용가능한 조약법(treaty law)이 존재하는 경우 이를 우선 적용하고, 조약이 존재하지 않을 경우 그리고 특히 식민지에서 해방된 신생국들과 관련된 경우에 기존 행정관할경계유지(uti possidetis) 원칙을 고려하 며, 앞서의 두 가지 준칙을 적용할 수 없는 경우에 대상이 된 영토 또는 지역에 대한 실효적 지배(effective control) 여부 또는 정도를 기준으로 권원의 존재와 영토주권의 귀속을 판단하고 있다. 국제법원의 사례를 보면 독도문제에 국제법적 근거로서 샌프란시스코 평화조약을 최우선으로 해석하여 적용할 가능성이 있으며 이러한 경우 당사국간에 분쟁의 해결은 물론 결과를 통한 평화 달성에 한계가 분명하다. 더욱이 샌프란시스코 평화조약은 동서냉전과 한국전쟁 그리고 한미일 군사동맹체제 등 당사국의 사활적 국가이익이라는 고도의 정치적 요인으로 인하여 조약에 의한 영토경계획정을 유보하였다. 그럼에도 불구하고 독도에 대한 언급을 의도적으로 배제한 조약을 기초로 국제법원이 사법적 판단을 하는 경우에 당해 법원은 샌프란시스코 조약뿐만 아니라 동 조약의 체결 이전에 이루어진 국제적 합의로서 카이로선언, 포츠담 선언, SCAPIN 677과 1033 등을 동 조약의 영토조항 해석의 기준으로 인정해야 할 것이다.

We investigated the effect of light spectra on circadian rhythm by exogenous prolactin (PRL) by using light emitting diodes (LEDs): red, green, and purple. We injected PRL into live fish or treated cultured brain cells with PRL. We measured changes in the expressions of period 2 (Per2), cryptochrome 1 (Cry1), melatonin receptor 1 (MT1) mRNAs, and MT1 proteins, and in the plasma PRL, serotonin, and melatonin levels. After PRL injection and exposure to green LED light, MT1 expression and plasma melatonin levels were significantly lower, but the expressions of Per2 and Cry1 were significantly higher than others. Plasma serotonin after PRL injection and exposure to red LED light was significantly lower than others. These results indicate that injection of high concentration PRL inhibits melatonin, and inhibited melatonin regulates circadian rhythm via clock genes and serotonin. Thus, exogenous PRL regulates the circadian rhythm and light spectra influence the effect of PRL in goldfish.