In buckwheat, interspecific hybrids with perennial species could provide the opportunity to introgress new and useful characters into annual species. An in vitro embryo rescue procedure, previously developed to produce interspecific hybrids of selfpollina

The isolation of female gametes is precondition for the satisfactory micromanipulation of gametes in common buckwheat (Fagopyrum esculentum Moench.). Embryo sacs were isolated at flowering stage after brief treatment with minimal concentrations of cell-wa