We investigate ρ Pup using the high resolution spectral observations taken from the VLT archive and observations at a 1.8m-Korean telescope with BOES spectrograph. The atmospheric parameters are determined using the iron-line abundance analysis. We derive an effective temperature value of Teff=6890±250K, surface gravity of log g=3.28±0.3 dex, microturbulent velocity of vmicro=4.1±0.4 km s-1, and the iron abundance of log N=7.82±0.15. The projected rotational velocity of the star is close to v sin i =3.5km s-1. Asymmetric line profiles in the observed spectra and variation of this asymmetry with time show that both strong radial pulsation and weak non-radial pulsations are present in ρ Pup