This study was to investigate the sorbic acid, benzoic acid and sulfur dioxide in commonly consumed beverages, snacks and instant ramens in Korea. A total of 150 food samples including 50 beverages, 50 snacks and 50 instant ramens were examined using the Korea Food Code method. Sorbic and benzoic acid were analyzed by the HPLC method, whereas sulfur dioxide was measured by Monnier-Williams method. Our results indicated that benzoic acid was detected in six beverages samples, and its concentration was in the range of 3.08-11.94 mg/kg. The contents of both sorbic and benzoic acid in 50 beverage samples did not exceed the residue allowance standards set by the Ministry of Food and Drug Safety (MFDS). Sulfur dioxide was detected in 12 beverages samples, but its content was lower than the detection limit specified in the method by the Korea Food Code. On the other hand, sorbic acid was not detected all samples. These results provide a basic data regarding sorbic acid, benzoic acid and sulfur dioxide in commonly consumed beverages, snacks and instant ramens in Korea.

견과류 중에 있는 트리코테센계 곰팡이독소 오염도를 조사하기 위하여 LC-MS/MS를 이용한 정확성과 신뢰성을 동시에 확보할 수 있는 분석방법을 개발하였다. 견과류 중 트리코테센계 곰팡이 독소는 QuEChERS 추출 및 EMRLipid- dSPE 정제과정을 통하여 분석에 사용되었다. 검량선 작성을 위하여 트리코테센계 곰팡이독소 10종에 대하여 2.00~75.00 μg/kg의 범위로 혼합표준용액을 제조하여 실험 하였으며, 상관계수는 모두 0.998 이상으로 높은 직선성을 나타내었다. 분석방법의 검출한계는 0.41~3.57 μg/kg로 나타났으며, 정량한계는 1.23~10.82 μg/kg로 나타났다. 또한 트리코테센계 곰팡이 독소 10종에 대하여 각각 저, 중, 고 3가지 농도로 처리하여 회수율 실험을 수행한 결과 81.84~ 96.87%로 나타났다. 확립된 분석법으로 견과류 중 땅콩을 대상으로 오염도를 조사한 결과 1종에서 deoxynivalenol이 검출되었다. 이러한 결과를 바탕으로 확립된 시험법은 견과류 중 트리코테센계 곰팡이 독소 분석에 적합함을 확인 할 수 있었으며 견과류 중 트리코테센계 곰팡이 독소 검출 가능성을 확인한바 보다 다양한 종류의 견과류에 대한 모니터링 조사가 필요한 것으로 판단된다.

Objective of this study was to investigate the residual levels of heavy metals in rice contract farming complex for exporting to China. Paddy soil, irrigation water and rice grain were taken from 20 fie1ds located in Icheon city, Cheorwon-gun, Cheongju city, Seocheon-gun, Gunsan city and Haenamgun. The elements of samples were analyzed using ion chromatography, ICP-OES, and ICP-MS after acid digestion. The arsenic (As) contents of paddy soil were ranged from 2.9 to 18.2 mg/kg, which were lower than 25 mg/kg as concern level of environmental pollution in Korea. Cadmium (Cd) was below the limit of quantitation (0.006 mg/kg) in all samples. The highest contents of copper (Cu) was detected to be 25.6 mg/kg in Seocheon-gun sample, but it was below 1/10 fold of the threshold levels 250 mg/kg for soil pollution. Also, the average contents of nikel (Ni), lead (Pb), zinc (Zn), and hexavalent chrome (Cr6+) were found to be lower than the criterion of soil pollution concern, and it was considered to be safe. The residual levels of arsenic in agricultural waters were relatively high, up to 24.3 ug/L in river water, but was detected as 1~2 ug/L level in the ground water. These levels are lower than the water quality standard, 0.05 mg/kg of agricultural water. The concentrations of mercury (Hg) and total chromium (Cr) in the white rice and brown rice were less than the limit of quantitation, and the levels of cadmium in the range of 0.004 to 0.068 mg/kg were less than safety criteria 0.2 mg/kg in Korea and China. In addition, the contents of lead (Pb) in white rice ranged from 0.002 to 0.136 mg/kg, which was safe to be 0.2 mg/kg for Korean white rice and 0.2 mg/kg for China brown rice. As a whole, the residual levels of heavy metals such as arsenic in rice was safely maintained as 1/10 to 1/20 of the residual limits of Korea and China. In conclusion, the heavy metals levels should not be worried in rice contract farming complex for exporting to China.

Wild grass is edible, and it grows in the mountains or field areas. Wild grass has diverse biological effects, such as antiobesity, anti-cancer, antioxidant activities and immune stimulation. Currently, many studies are aimed at enhancing the efficacy of medicinal foods on biological activity using a bioconversion technology, including the fermentation process. In this study, the quality characteristics and antioxidative activity of the fermented wild grass was investigated. The antioxidant activity of fermented wild grass was assessed by various radical scavenging assays using DPPH(2, 2-diphenyl-1-picrylhydrazyl), FRAP(ferric ion reducing antioxidant power), reducing power, and ABTS (2, 2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)). Moisture contents of the fermented wild grass is 49.6±0.06%. Contents of crude ash, crude protein, and crude fat were 0.65±0.01, 0.65±0.04, and 3.3±0.59%, respectively. Moreover, fermented wild grass showed that the hunter's color values were 80.36(lightnees), 11.47(redness), and 44.53(yellowness), respectively. Total phenolic contents of the fermented wild grass was 1, 185±159 ㎍ GAE(gallic acid equivalent)/g. The antioxidative activities of the fermented wild grass were significantly increased in a dose dependent manner. In addition, fermented wild grass did not show any cytotoxicity up to 500 ㎍/㎖. However, the anti-adipogenic effect of the fermented wild grass extract was barely detectable. This antioxidant potential is partly due to the phenolic compounds that are present in the fermented wild grass extracts.

Ski protein is a nuclear transcription factor that does not bind DNA directly. Due to its unique binding properties with multiple factors, Ski could perform various roles in the regulation of both cellular proliferation and differentiation. We had previously reported that Ski protein is present in granulosa cells of atretic follicles, but not in preovulatory follicles, suggesting that Ski has a role in apoptosis of granulosa cells. The alternative fate of granulosa cells other than apoptosis is to differentiate to luteal cells; however, it is unknown whether Ski is expressed and has a role in granulosa cells undergoing luteinization. Thus, the aim of the present study was to examine whether the initiation of luteinization with luteinizing hormone (LH) directly regulates expression of Ski in the luteinized granulosa and luteal cells after ovulation by in vitro models. RT-PCR and real time PCR analysis respectively revealed that LH had no effect on c-Ski mRNA expression in the cultured granulosa cells regardless of LH treatment. Though Ski protein isabsent in granulosa cells of preovulatory follicle, its mRNA(c-Ski) was expressed and the level was unchanged even after LH surge. Taken together, these results demonstrated that Ski protein expression is induced in granulosa cells upon luteinization, and suggested that its expression is regulated post-transcriptionally. Moreover, expression of mRNA of Arkadia, an E3 ubiquitin ligases, in luteinizing granulosa cells in vivo was assessed by real time-PCR. The levels of Arkadia mRNA expression were unchanged during follicular growth and post ovulatory luteinization. These findings suggest that Ski protein level may be regulated during luteinization at translational and/or post-translational level but not by Arkadia.

본 연구에서는 강내한성 강원양파의 건조방법을 달리하 여 건조방법에 따른 이화학적 특성을 비교하였다. FD, HD 40 및 HD 60의 세 가지 분말에 대해 일반성분 분석, 색도, 갈색도, 총 페놀 함량 및 DPPH free radical 소거능을 측정 하였다. 일반성분 분석의 결과 FD의 조단백질 및 조지방의 함량이 각각 10.99%, 0.84%로 HD 40 및 HD 60 보다 유의적 으로 높게 나타났다. 색도의 경우, Hunter's value로 L값 (lightness), a값(redness), b값(yellowness)으로 나타내었다. FD의 L값이 77.19±0.01로 가장 높게 나왔으며, 나머지 a값 및 b값은 각각 6.09±0.01과 24.60±0.03으로 HD 60이 높게 나왔다. 갈변도의 경우에도 HD 60이 0.200으로 제일 높은 값으로 나타나 건조 온도가 높아질수록 양파의 갈변정도가 커진 다는 것으로 사료된다. 총 페놀 함량의 경우에 HD 40과 HD 60이 FD 보다 더 높은 함량을 나타내었다. DPPH radical 소거능은 시료의 농도를 0.1, 0.5, 1 mg/mL로 처리하 여 측정한 결과 농도에 따라 유의적으로 항산화 활성이 증가되었으며, FD 보다 HD 40 및 HD 60에서 유의적으로 더 높은 항산화 활성을 나타내었다.