Genetic diversity plays a great role in the survival and adaptability of a species under changable environment. Korea is known as original region of cultivated soybean species with high genetic diversity of wild and cultivated soybean lines. We have collected many semi-wild soybean lines, however, the information about the genetic diversity of these lines is limited. And also the phylogenetic relationship and genetic distance among the semi-wild, typical wild types (Glycine soja Sied. & Zucc.), and cultivated soybean(Glycine max (L.) Merr.) have been controversial. This study was carried out to analyze the diversity of the three groups, and to verify their genetic variation and relationships among them. The diversity indexes of typical wild, semi-wild, and cultivated soybean accessions were 0.854, 0.677, and 0.528, respectively. According to the cluster analysis for the genetic relationships, 58 accessions were defined into three genetic groups. Cluster I consisted of 20 cultivated soybean accessions and three semi-wild soybean lines. Cluster II contained 13 semi-wild soybean lines and one typical wild soybean line. Cluster II included 21 typical wild soybean lines and one semi-wild soybean line. Genetic distance between cultivated and semi-wild soybean, typical wild and semi-wild, and cultivated and typical wild were 0.525 0.761 and 0.810, respectively. The relationship between semi-wild soybean was much closer to the cultivated soybean the to the wild soybean