In existing ceramic mold manufacturing processes, inorganic binder systems (Si-Na, two-component system) are applied to ensure the effective firing strength of the ceramic mold and core. These inorganic binder systems makes it possible to manufacture a ceramic mold and core with high dimensional stability and effective strength. However, as in general sand casting processes, when molten metal is injected at room temperature, there is a limit to the production of thin or complex castings due to reduced fluidity caused by the rapid cooling of the molten metal. In addition, because sodium silicate generated through the vitrification reaction of the inorganic binder is converted into a liquid phase at a temperature of 1,000 °C. or higher, it is somewhat difficult to manufacture parts through high-temperature casting. Therefore, in this study, a high-strength ceramic mold and core test piece with effective strength at high temperature was produced by applying a Si-Na-Ti three-component inorganic binder. The starting particles were coated with binary and ternary inorganic binders and mixed with an organic binder to prepare a molded body, and then heat-treated at 1,000/1,350/1,500 °C to prepare a fired body. In the sample where the two-component inorganic binder was applied, the glass was liquefied at a temperature of 1,000 °C or higher, and the strength decreased. However, the firing strength of the ceramic mold sample containing the three-component inorganic binder was improved, and it was confirmed that it was possible to manufacture a ceramic mold and core via high temperature casting.

황련은 한의학에서 진정, 소염, 항균 및 해열 등의 여러 질병의 치료에 이용되어져 왔다. 본 연구에서는 UVB와 TNF-α/IFN-γ로 유도된 HaCaT 세포에서 피부장벽과 염증관련 인자에 대한 황련 열수 추출물(CCW)의 효과를 조사하고, 보습 및 항염증 소재로서의 가능성을 알아보고자 하였다. 각 질형성세포인 HaCaT 세포에서 CCW의 보습 및 항염 효과를 확인하기 위해 MTT assay를 통한 시료의 독성 평가를 실시하였다. 이를 바탕으로, HA(Hyaluronic acid) 생성량과 filaggrin의 protein 및 mRNA 발현을 측정하였다. TNF-α/IFN-γ로 자극된 HaCaT 세포에서 CCW는 농도 의존적으로 HA 의 생성량을 증가 시켰고, filaggrin의 protein 및 mRNA 발현 측정 결과 CCW의 농도 증가에 따라 발현율도 증가하였다. UVB로 유도된 HaCaT 세포에서 CCW는 ROS의 생성을 감소 시켰고, 양성대조군 (positive control)인 EGCG( (-)-epigallocatechin-3-gallate)와 유의한 결과를 보여 주었다. 또한, CCW 는 염증성 사이토카인 TNF-α, IL-1β, IL-6, IL-8의 발현을 억제 시켰고, TNF-α/IFN-γ로 자극 된 HaCaT 세포에서 피부 염증에 따른 주요 인자인 COX-2의 protein 및 mRNA 발현이 농도 의존적으로 감소되는 것을 확인할 수 있었다. 이러한 결과는, 황련 열수 추출물의 보습 및 항염증 효과를 가지 는 화장품 소재로서의 가치를 제안할 수 있다.

2018년과 2019년에 서울에서 유통 중인 건고추 및 고춧가루에 대해 잔류 농약 안전성 검사를 실시하였다. 총 101건의 시료에 대해 71종 농약을 모니터링한 결과 잔류허 용기준을 초과한 시료는 없었으나 잔류농약이 검출된 시료는 87건으로 86.1%의 검출률을 나타내었다. 건고추와 고춧가루 검출률은 각각 73.3%, 91.5% 이었다. 고춧가루의 잔류농약 검출률이 건고추에 비해 다소 높게 나타났 다. 검출된 농약은 12종이었으며 모두 작물보호제 지침 서에 따른 고추에 사용가능한 살균제 및 살충제이었다. 가장 다빈도로 검출된 농약은 pyraclostrobin이었으며 다음으로 flubendiamide, azoxystrobin, chlorantraniliprole 순이었다. 검출된 농약에 대한 위해성을 ADI(Acceptable daily intake)대비 식이섭취율로 산출하여 평가한 결과, %ADI는 모두 5.66E-05 – 3.34E-02%로 나타나 안전한 것으로 나타났다.

다수호기 부지에 위치한 원전의 해체는 인근에 위치한 운영중인 원전으로 인해 작업자에게 추가적인 방사선 피폭 위험을 야기할 수 있다. 따라서 인근의 운영중인 다수호기 원전에 의한 해체 작업자에 대한 피폭 선량 평가가 필요하다. ENDOS프로그램은 한국원자력연구원(KAERI)에서 개발된 선량평가 전산코드로, 하위 프로그램으로 대기 확산 평가 프로그램인 ENDOSATM과 기체 방사성 배출물에 의한 피폭 선량 계산을 수행하는 ENDOS-G가 있다. 이 프로그램들을 이용하여 고리 1호기 해 체작업자에 대한 다수호기 원전 운영에 의한 피폭 선량을 계산한 결과, 2.31×10-3 mSv·y-1로 일반인에 대한 피폭선량 기준치인 1 mSv·y-1에 비교해 보았을때 큰 영향이 없을 것이라는 판단에 도달할 수 있었다. 앞으로 예상되는 국내 해체 원전의 경우 모두 다수호기 부지에 위치하여 이 연구 방법과 결과가 활용될 수 있을 것이라고 기대한다.

Shiitake culture in sawdust is a widely applied method, which can supplement the disadvantages of costly and time consuming oak log cultivation. In sawdust cultivation, browning of surface mycelia is an important stage for the productivity and longevity of sawdust media. Surface browning forms protection coat for the substrate, which can block the invasion of outer pathogens and suppress water evaporation in the substrate. We controlled different light source (red LED, white LED, blue LED, and fluorescent light) with different intensity of illumination (1.5, 10.5, 20.5 μmol/m2s for LEDs and 10, 100, 300 lux for fluorescent light) to induce browning. Lights were treated with 1 hour on/ 1 hour off cycle maintained in a controlled room with 20℃ temperature, 60% humidity, and 1200 ppm CO2 atmosphere concentration for 60 days. Browning effect differed from the source and intensity of illumination. Browning was most effective in 1.5 μmol/m2s for red and blue LED. All light sources showed less browning in highest intensity of illumination, which indicates that higher than 20.5 μmol/m2s for LEDs or 300 lux for fluorescent light are not effective. After harvesting fruit bodies, we measured their weight, length and width of pileus and stipe, chromaticity, and hardness. Treatment with 1.5 μmol/m2s blue LED produced the best harvest with highest average individual weight (21.2g), stipe length (30.8 mm), and hardness (377.9 g) with fine length and width of pileus, and chromaticity. This results indicate that 1.5 μmol/m2s blue LED showed the best browning effect which resulted in the best harvest yield.

Wolfiporia cocos is a widely known traditional medicine in China, Japan, Korea, and other Asian countries due to its various medicinal effect. Aiming to determine the optimum condition for W. cocos fruit body induction, we cultured 10 strains of W. cocos on potato dextrose agar medium (PDA) in different temperature conditions (12, 16, 20, 24, 28oC). KFRI 1105 exceptively formed fuitbody in low temperatures (12 and 16oC), but in other strains, fruit body induction was restricted. in 12 and 16oC. Fruit body formation started 20oC, and formation rate increased proportionally with the temperature increase. 5 strains induced fruit body in 20oC, 7 strains in 24oC, and 9 strains in 28oC. This is the first research to identify a fruit body formation in vitro cultured W. cocos in Korea. This investigation will enable further studies of W. cocos physiology and breeding. In summary : 1. We identified the optimal conditions for W. cocos fruit body induction in the PDA plate. 2. The fruit body was best induced when it was cultured in 28 oC for 32 days. 3. The fruit bodies showed a honeycomb-like structure, and the average spore size was 7.55 μm in height and the average width was 3.35 μm.

Hepatocellular carcinoma (HCC) is a representative inflammation-associated cancer and known to be the most frequent tumor. However, the preventive agents for hepatocarcinogenesis are unsatisfactorily identified. We investigated the protective effect of steamed and freeze-dried mature silkworm larval powder (SMSP) on diethylnitrosamine (DEN)-induced hepatotoxicity in mice and compared the effect of three silkworm varieties: white-jade, golden-silk, and light-green strains. The mice were fed with diet containing 0.1, 1, and 10 g/kg of three types of SMSP for two weeks while DEN (100 mg/kg, i.p.) was injected 18 h before the end of this experiment. Liver toxicity was determined as serum indicator, histopathological examination, and expression of inflammatory enzyme. Pretreatment with SMSP reduced necrotic and histopathological changes induced by DEN in the liver. The measurement of serum biochemical indicators showed that pretreatment with SMSP also decreased DEN-induced hepatotoxicity, the levels of alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST). In addition, SMSP inhibited the expressions of inflammatory enzymes, cyclooxygenase-2 and inducible nitric oxide synthase. White-jade SMSP showed the most effective hepatoprotective results against hepatotoxicity among the three silkworm strains used in this study. SMSP may have a protective effect against acute liver injury by inhibiting necrosis and inflammatory response in DEN-treated mice.

This study optimized physicochemical factors for the sphericity of capsule type-fish roe analogs using equipment with double nozzles. The manufacturing process of fish roe analogs was optimized by response surface methodology with central composite design. For the optimum conditions of sphericity, sodium alginate concentration, calcium chloride concentration, agitation speed of calcium chloride solution in a reactor, and dropping height were investigated as independent variables. Soybean oil was put into the equipment through an internal nozzle, along with sodium alginate solution through an external nozzle into calcium chloride solution. The optimum conditions for the production of fish roe analogs were 1.66% (w/v) sodium alginate, 1.86% (w/v) calcium chloride, agitation speed of calcium chloride at 280 rpm, dropping height at 17 cm between the tip of the nozzle and the surface of calcium chloride solution. Consequently, the fish roe analogs showed a high sphericity of 99.8±0.77% at optimal conditions.

We have investigated the influence of system composition and preparation conditions on the particle size of vitamin E acetate (VE)-loaded nanoemulsions prepared by PIC(phase inversion composition) emulsification. This method relies on the formation of very fine oil droplets when water is added to oil/surfactant mixture. The oil-to-emulsion ratio content was kept constant (5 wt.%) while the surfactant-to-oil ratio (%SOR) was varied from 50 to 200 %. Oil phase composition (vitamin E to medium chain ester ratio, %VOR) had an effect on particle size, with the smallest droplets being formed below 60 % of VOR. Food-grade non-ionic surfactants (Tween 80 and Span 80) were used as an emulsifier. The effect of f on the droplet size distribution has been studied. In our system, the droplet volume fraction, given by the oil volume fraction plus the surfactant volume fraction, was varied from 0.1 to 0.3. The droplet diameter remains less than 350 nm when O/S is fixed at 1:1. The droplet size increases gradually as the increasing the volume fraction. Particle size could also be reduced by increasing the temperature when water was added to oil/ surfactant mixture. By optimizing system composition and homogenization conditions we were able to form VE-loaded nanoemulsions with small mean droplet diameters (d < 50 nm). The PIC emulsification method therefore has great potential for forming nanoemulsion-based delivery systems for food, personal care, and pharmaceutical applications.

소염·진통 의약품 성분인 beclomethasone, dexamethasone, prednisolone, ketoprofen, phenylbutazone 5종에 대 한 동시분석을 위해 LC-MS/MS 분석 조건 중 MRM 방식 으로 각 성분의 MS 분석 최적조건을 결정하고, 정량이온 으로 beclomethasone 409.1/391.0, dexamethasone은 393.1/ 372.9, prednisolone은 361.0/343.1, ketoprofen은 255.0/209.0, phenylbutazone은 309.1/160.1 을 분석하였다. 혼합표준용 액을 기울기용매 조건으로 이동상 A(0.1% 개미산), B(0.1% 개미산을 함유한 아세토니트릴)를 이용하여 17분 동안 분 석한 결과, prednisolone (tR: 7.34 min), dexamethasone (tR: 7.73 min), beclomethasone (tR: 7.82 min), phenylbutazone (tR: 8.31 min), ketoprofen (tR: 9.24 min) 순서로 검출되었 다. 5종 성분 모두 약 2-100 ng/mL 농도 수준의 검정곡선 에서 R2 값이 0.999 이상의 우수한 직선성을 나타내었고, prednisolone을 제외한 4종 성분의 검출한계는 0.4-0.9 ng/ mL, 정량한계는 0.81-2.22 ng/mL 였으며, prednisolone은 그보다 다소 높은 4.60, 11.46 ng/mL 이었다. 환제품의 기 타가공품 공시료에 5종 성분 혼합표준용액을 최종농도가 5, 20, 50 ng/mL 이 되도록 직접 첨가하여 분석한 결과, 모든 농도에서 80% 이상의 우수한 회수율을 얻을 수 있 었고, 일내 일간 반복 분석의 상대표준편차(%)를 통해 모 든 성분에서 비교적 재현성 있는 결과가 나타났다. 실제 인터넷 상에서 유통중인 식품에 이 분석법을 적용한 결과 모든 제품에서 검출되진 않았으나, 소비자들의 건강상 위 해를 끼칠 수 있는 이들 성분의 동시분석법을 활용한 지 속적인 모니터링이 필요한 실정이다.

Vitamin C (ascorbic acid) is an essential nutrient of most living tissues. We established a strain of Gulo-/- mice with known deficiency, in which vitamin C intake can be controlled by diet, like humans, and investigated the differen- tially expressed proteins following treatments with Helicobacter pylori and diethylnitrosamine (DENA) in the liver of Gulo-/- mice using a proteomic approach. Expression of p53, 14-3-3ε and 14-3-3δ in Gulo-/- mice liver tissue was analyzed by immunohistochemistry. 2-DE maps constructed from Gulo-/- mice liver and differentially expressed proteins in liver tissue were identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF/MS). In Gulo- /- mice after H. Pylori infection, followed by treatment with DENA, no differences in p53, 14-3-3ε and 14-3-3δ were observed by immunohistochemistry. Proteome analyses us- ing MALDI-TOF/MS resulted in successful identification of 12 proteins (nine proteins were up-regulated and three were down-regulated). Specifically, peroxiredoxin-6 and Alpha-1-antitrypsin 1-4 were up-regulated in liver after H. Pylori infection followed by treatment with DENA. These results indicated that oral supplementation with vitamin C led to rescue of Gulo-/- mice from vitamin deficiency, and protected the liver from H.pylori infection and/or DENA ef- fect, and vitamin C also protected the liver against oxidative stress.

Fraxinus rhynchophylla (Oleaceae), a deciduous tree, is known to have properties that include anti-inflammatory, convergence, febricide, antiblenophthalmia, antidiarrhea, antileukorrhea, and so forth. In addition, it has been used for traditional herbal medicine in East Asian countries, including Korea. In this study, we investigated the antioxidant and anti-inflammatory effects of Fraxinus rhynchophylla ethanol extract (FRE) in lipopolysaccharide (LPS)-induced murine macrophage Raw 264.7 cells with FRE pretreatment. We performed DPPH-assay, Western blot, and Reverse Transcription-Polymerase Chain Reaction (RT-PCR). FRE showed 85% free radical scavenging activity at concentrations of 80 µg/ml. Results of this study also showed that FRE down-regulates Cox-2 and iNOS expression in mRNA and protein level. In conclusion, crude ethanol extract of Fraxinus rhynchophylla exhibited antioxidant and anti-inflammatory activities, and it may potentially provide a valuable source of natural herbal agent to inhibit inflammation.

This study was performed to investigate the antifeeding activity of Perilla frutescens extracts against diamondback moth, Plutella xylostella larvae and to confirm the electrophysiological responses of two sensilla (LST=lateral styloconic sensillum, MST=medial styloconic sensillum) in maxilla galea, a chemoreceptor. Crude extract of P. frutescens in methanol was showed antifeeding activity approximately 70%, and subsequently separated into four fractions - n-hexane(H), chloroform(C), ethylacetate(E), and water(W). Antifeeding activity was only showed in n-Hexane fraction around 99%. H1, H2, H3, H4, H5 were isolated from n-Hexane fraction using an open column chromatography, and the bioassay showed strongest antifeeding activity in H1 fraction. Using an oscilloscope, electrophysiological responses of two sensilla showed more seven times activity in MST. H11, H12, H13, H14 were separated from H1 fraction and antifeeding activity was showed highest in H11 fraction. H11 fraction was examined electrophysiological responses at doses of 100, 10, and 1 ppm, and MST of P. xylostella responded at a dose of 100 ppm. H11 fraction was separated using HPLC and identified using GC/MS and NMR. Finally, the structure of active compound proved to be farnesene with molecular weight of 204, and a formula of C15H24

This study purposed to examine how the window display of flower shops affects consumers' buying desire, and for this purpose, we surveyed flower buying customers' interest in window display according to their gender, age, and occupation. The survey was conducted using a questionnaire with flower shop customers in Seoul, Daegu, and Gyeongju. Interest in window display was over 1.5 times higher in women than in men. Among criteria for choosing a flower shop, window display was more important than other factors. With regard to experience of being stopped by unique window display, many of the respondents replied that they had such an experience, and the frequency was higher among women than among men. Among different types of window display, theme-type window display was most effective in stimulating customers' curiosity and buying desire. According to occupation, housewives showed particularly high interest in window display. The results of this study show that the window display of flower shops has a significant effect on consumers' buying desire.